Characterization of Exopolysaccharides from <i>Lactiplantibacillus plantarum</i> PC715 and Their Antibiofilm Activity Against <i>Hafnia alvei</i>
Exopolysaccharides (EPSs) secreted by lactic acid bacteria have the potential to enhance human health by showing various biological functions. This study investigated the biological role and antibiofilm properties of EPS715, a new neutral EPS produced by pickled vegetables originating from <i>...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/11/2229 |
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| Summary: | Exopolysaccharides (EPSs) secreted by lactic acid bacteria have the potential to enhance human health by showing various biological functions. This study investigated the biological role and antibiofilm properties of EPS715, a new neutral EPS produced by pickled vegetables originating from <i>Lactobacillus plantarum</i> PC715. The results indicate that EPS715 is primarily composed of rhamnose, glucose, and mannose. Its molecular weight (Mw) is 47.87 kDa, containing an α-glucoside linkage and an α-pyranose ring. It showed an amorphous morphology without a triple helix structure. Furthermore, EPS715 showed improved antioxidant activity. Specifically, its scavenging capacity of ABTS<sup>+</sup> radicals, DPPH radicals, and the hydroxyl (·OH) reduction capacity at 5 mg/mL was 98.64 ± 2.70%, 97.37 ± 0.79%, and 1.64 ± 0.05%, respectively. Its maximal scavenging capacity was >40%, and the hydroxyl (·OH) radical scavenging ability was dose-dependent. Moreover, the biofilm of various pathogens including <i>S. aureus</i>, <i>B. cereus</i>, <i>S. saprophyticus</i>, <i>Acinetobacter</i> spp., and <i>H. alvei</i> was substantially dispersed and affected by EPS715, with a maximum inhibition rate of 78.17% for <i>H. alvei</i>. The possible mechanism by which EPS715 shows antibiofilm properties against the <i>H. alvei</i> may be attributed to its effects on the auto-aggregation, hydrophilic characteristics, and motility of <i>Hafnia</i> spp. Thus, EPS715 has significant antioxidant and antibiofilm characteristics that may hold substantial potential for applications in food and medicinal products. |
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| ISSN: | 2076-2607 |