T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity
Objective: Ankylosing spondylitis (AS) is a chronic autoimmune disease characterized by systemic inflammation, often resulting in fusion of the spine and peripheral joints. This study aimed to investigate the role of innate lymphoid cells (ILCs) in AS patients with high disease activity. Methods: Bl...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025000581 |
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| author | Yang Liu Yi Shen Hongbai Ding Dongyi He Peng Cheng Xinyao Wu Zheng Xiang Lei Shen Yanqin Bian Qi Zhu |
| author_facet | Yang Liu Yi Shen Hongbai Ding Dongyi He Peng Cheng Xinyao Wu Zheng Xiang Lei Shen Yanqin Bian Qi Zhu |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Objective: Ankylosing spondylitis (AS) is a chronic autoimmune disease characterized by systemic inflammation, often resulting in fusion of the spine and peripheral joints. This study aimed to investigate the role of innate lymphoid cells (ILCs) in AS patients with high disease activity. Methods: Blood samples were collected from healthy controls and AS patients categorized by high or low disease activity. Systemic inflammation was quantified through C-reactive protein (CRP) levels and erythrocyte sedimentation rate (ESR), alongside disease activity scores such as Ankylosing Spondylitis Disease Activity Score(ASDAS) and Bath Ankylosing Spondylitis Disease Activity Index(BASDAI). The levels of different ILC subsets and the expression of T-box transcription factor 21 (T-bet) and retinoic-acid-receptor-related orphan receptor gamma (RORγt) in peripheral blood were analyzed via flow cytometry. Additionally, 24 cytokines in plasma were measured using a Luminex liquid suspension chip. Results: The proportion of total ILCs and the distribution of ILC subsets in peripheral blood varied with AS disease activity scores. Specifically, the frequencies of total ILCs and ILC3s were significantly elevated in AS patients with high disease activity (AS-HA). The frequency and absolute number of ILC3s showed a positively correlation with disease severity scores. Furthermore, T-bet+ILC3s were significantly increased in the AS-HA group. Plasma levels of IL-17A and IFN-γ were positively correlated with the frequency of circulating-ILC3 in AS patients. Conclusions: These findings highlight the critical role of T-bet+ILC3s in the inflammatory process of AS, suggesting their potential as a therapeutic target for managing AS disease. |
| format | Article |
| id | doaj-art-b82b73e289d647d38f3d6c5d562d96bb |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-b82b73e289d647d38f3d6c5d562d96bb2025-01-17T04:51:53ZengElsevierHeliyon2405-84402025-01-01111e41678T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activityYang Liu0Yi Shen1Hongbai Ding2Dongyi He3Peng Cheng4Xinyao Wu5Zheng Xiang6Lei Shen7Yanqin Bian8Qi Zhu9Department of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, China; Department of Traditional Chinese Medicine, PengPu Town No.2 Community Healthcare Center, Shanghai, 200436, ChinaDepartment of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, China; Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, ChinaDepartment of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, ChinaDepartment of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, China; Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, ChinaDepartment of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, ChinaDepartment of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, ChinaInstitute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, and Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaInstitute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, China; Corresponding author. Institute of Arthritis Research in Integrative Medicine Shanghai Academy of Traditional Chinese Medicine, Shanghai, China.Department of Rheumatology, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200052, China; Institute of Arthritis Research in Integrative Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200052, China; Corresponding author. Department of Rheumatology, Guanghua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai, China.Objective: Ankylosing spondylitis (AS) is a chronic autoimmune disease characterized by systemic inflammation, often resulting in fusion of the spine and peripheral joints. This study aimed to investigate the role of innate lymphoid cells (ILCs) in AS patients with high disease activity. Methods: Blood samples were collected from healthy controls and AS patients categorized by high or low disease activity. Systemic inflammation was quantified through C-reactive protein (CRP) levels and erythrocyte sedimentation rate (ESR), alongside disease activity scores such as Ankylosing Spondylitis Disease Activity Score(ASDAS) and Bath Ankylosing Spondylitis Disease Activity Index(BASDAI). The levels of different ILC subsets and the expression of T-box transcription factor 21 (T-bet) and retinoic-acid-receptor-related orphan receptor gamma (RORγt) in peripheral blood were analyzed via flow cytometry. Additionally, 24 cytokines in plasma were measured using a Luminex liquid suspension chip. Results: The proportion of total ILCs and the distribution of ILC subsets in peripheral blood varied with AS disease activity scores. Specifically, the frequencies of total ILCs and ILC3s were significantly elevated in AS patients with high disease activity (AS-HA). The frequency and absolute number of ILC3s showed a positively correlation with disease severity scores. Furthermore, T-bet+ILC3s were significantly increased in the AS-HA group. Plasma levels of IL-17A and IFN-γ were positively correlated with the frequency of circulating-ILC3 in AS patients. Conclusions: These findings highlight the critical role of T-bet+ILC3s in the inflammatory process of AS, suggesting their potential as a therapeutic target for managing AS disease.http://www.sciencedirect.com/science/article/pii/S2405844025000581Innate lymphoid cellsAnkylosing spondylitisTranscription factor T-betInflammation |
| spellingShingle | Yang Liu Yi Shen Hongbai Ding Dongyi He Peng Cheng Xinyao Wu Zheng Xiang Lei Shen Yanqin Bian Qi Zhu T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity Heliyon Innate lymphoid cells Ankylosing spondylitis Transcription factor T-bet Inflammation |
| title | T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| title_full | T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| title_fullStr | T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| title_full_unstemmed | T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| title_short | T-bet+ILC3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| title_sort | t bet ilc3 in peripheral blood is increased in the ankylosing spondylitis with high disease activity |
| topic | Innate lymphoid cells Ankylosing spondylitis Transcription factor T-bet Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025000581 |
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