Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time
Abstract Ischemic stroke constitutes a multifaceted neurological affliction that spans various cellular types. Lack of dynamic chromatin accessibility data after stroke is one of the obstacles to understanding this process. To gain insights into the variations in transcriptional regulation among var...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04367-4 |
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| author | Ruolin Zhang Sicheng Guo Jie Zhou Xiumei Lin Ying Wang Yiqi Wang Muyang Li Kaichen Zhao Wendai Bao Ke Shui Chuanyu Liu Chang Liu Zhiqiang Dong |
| author_facet | Ruolin Zhang Sicheng Guo Jie Zhou Xiumei Lin Ying Wang Yiqi Wang Muyang Li Kaichen Zhao Wendai Bao Ke Shui Chuanyu Liu Chang Liu Zhiqiang Dong |
| author_sort | Ruolin Zhang |
| collection | DOAJ |
| description | Abstract Ischemic stroke constitutes a multifaceted neurological affliction that spans various cellular types. Lack of dynamic chromatin accessibility data after stroke is one of the obstacles to understanding this process. To gain insights into the variations in transcriptional regulation among various cell types subsequent to a stroke, we employed single-nucleus ATAC-seq to curate a chromatin accessibility compendium from the cerebral cortex of mice subjected to middle cerebral artery occlusion/reperfusion (MCAO/R). Tissue samples were collected at various time points including 0, 6, 12, 24 hours, and 7, 14 days post-reperfusion, in addition to Sham control group. We obtained 99,271 high-quality nuclei across nine cell types, thereby establishing the single-nucleus chromatin accessibility atlas. This atlas provides data for interpreting the regulatory mechanisms that pervade the continuum of ischemic stroke. The data presented herein constitutes a valuable resource for the comprehension of regulatory interplays within the pathology-afflicted cerebrum. |
| format | Article |
| id | doaj-art-b8171429353c494abe10ada18e08db86 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-b8171429353c494abe10ada18e08db862025-01-12T12:07:39ZengNature PortfolioScientific Data2052-44632025-01-0112111110.1038/s41597-025-04367-4Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across timeRuolin Zhang0Sicheng Guo1Jie Zhou2Xiumei Lin3Ying Wang4Yiqi Wang5Muyang Li6Kaichen Zhao7Wendai Bao8Ke Shui9Chuanyu Liu10Chang Liu11Zhiqiang Dong12Hubei Clinical Research Center of Central Nervous System Repair and Functional Reconstruction, Taihe Hospital, Hubei University of MedicineBGI GenomicsBGI ResearchBGI ResearchCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural UniversityHubei Clinical Research Center of Central Nervous System Repair and Functional Reconstruction, Taihe Hospital, Hubei University of MedicineCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural UniversityCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural UniversityCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural UniversityCollege of Biomedicine and Health, College of Life Science and Technology, Huazhong Agricultural UniversityBGI ResearchBGI ResearchHubei Clinical Research Center of Central Nervous System Repair and Functional Reconstruction, Taihe Hospital, Hubei University of MedicineAbstract Ischemic stroke constitutes a multifaceted neurological affliction that spans various cellular types. Lack of dynamic chromatin accessibility data after stroke is one of the obstacles to understanding this process. To gain insights into the variations in transcriptional regulation among various cell types subsequent to a stroke, we employed single-nucleus ATAC-seq to curate a chromatin accessibility compendium from the cerebral cortex of mice subjected to middle cerebral artery occlusion/reperfusion (MCAO/R). Tissue samples were collected at various time points including 0, 6, 12, 24 hours, and 7, 14 days post-reperfusion, in addition to Sham control group. We obtained 99,271 high-quality nuclei across nine cell types, thereby establishing the single-nucleus chromatin accessibility atlas. This atlas provides data for interpreting the regulatory mechanisms that pervade the continuum of ischemic stroke. The data presented herein constitutes a valuable resource for the comprehension of regulatory interplays within the pathology-afflicted cerebrum.https://doi.org/10.1038/s41597-025-04367-4 |
| spellingShingle | Ruolin Zhang Sicheng Guo Jie Zhou Xiumei Lin Ying Wang Yiqi Wang Muyang Li Kaichen Zhao Wendai Bao Ke Shui Chuanyu Liu Chang Liu Zhiqiang Dong Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time Scientific Data |
| title | Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| title_full | Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| title_fullStr | Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| title_full_unstemmed | Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| title_short | Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| title_sort | monitoring of single nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time |
| url | https://doi.org/10.1038/s41597-025-04367-4 |
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