Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas.
Multiple human diseases including cancer have been associated with a dysregulation in RNA splicing patterns. In the current study, modifications to the global RNA splicing landscape of cellular genes were investigated in the context of Epstein-Barr virus-associated gastric cancer. Global alterations...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0176880&type=printable |
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| author | Victoria E S Armero Marie-Pier Tremblay Andréa Allaire Simon Boudreault Camille Martenon-Brodeur Cyntia Duval Mathieu Durand Elvy Lapointe Philippe Thibault Maude Tremblay-Létourneau Jean-Pierre Perreault Michelle S Scott Martin Bisaillon |
| author_facet | Victoria E S Armero Marie-Pier Tremblay Andréa Allaire Simon Boudreault Camille Martenon-Brodeur Cyntia Duval Mathieu Durand Elvy Lapointe Philippe Thibault Maude Tremblay-Létourneau Jean-Pierre Perreault Michelle S Scott Martin Bisaillon |
| author_sort | Victoria E S Armero |
| collection | DOAJ |
| description | Multiple human diseases including cancer have been associated with a dysregulation in RNA splicing patterns. In the current study, modifications to the global RNA splicing landscape of cellular genes were investigated in the context of Epstein-Barr virus-associated gastric cancer. Global alterations to the RNA splicing landscape of cellular genes was examined in a large-scale screen from 295 primary gastric adenocarcinomas using high-throughput RNA sequencing data. RT-PCR analysis, mass spectrometry, and co-immunoprecipitation studies were also used to experimentally validate and investigate the differential alternative splicing (AS) events that were observed through RNA-seq studies. Our study identifies alterations in the AS patterns of approximately 900 genes such as tumor suppressor genes, transcription factors, splicing factors, and kinases. These findings allowed the identification of unique gene signatures for which AS is misregulated in both Epstein-Barr virus-associated gastric cancer and EBV-negative gastric cancer. Moreover, we show that the expression of Epstein-Barr nuclear antigen 1 (EBNA1) leads to modifications in the AS profile of cellular genes and that the EBNA1 protein interacts with cellular splicing factors. These findings provide insights into the molecular differences between various types of gastric cancer and suggest a role for the EBNA1 protein in the dysregulation of cellular AS. |
| format | Article |
| id | doaj-art-b8079d9571ad46a1a1c3dfc7538f5971 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-b8079d9571ad46a1a1c3dfc7538f59712025-01-17T05:32:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017688010.1371/journal.pone.0176880Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas.Victoria E S ArmeroMarie-Pier TremblayAndréa AllaireSimon BoudreaultCamille Martenon-BrodeurCyntia DuvalMathieu DurandElvy LapointePhilippe ThibaultMaude Tremblay-LétourneauJean-Pierre PerreaultMichelle S ScottMartin BisaillonMultiple human diseases including cancer have been associated with a dysregulation in RNA splicing patterns. In the current study, modifications to the global RNA splicing landscape of cellular genes were investigated in the context of Epstein-Barr virus-associated gastric cancer. Global alterations to the RNA splicing landscape of cellular genes was examined in a large-scale screen from 295 primary gastric adenocarcinomas using high-throughput RNA sequencing data. RT-PCR analysis, mass spectrometry, and co-immunoprecipitation studies were also used to experimentally validate and investigate the differential alternative splicing (AS) events that were observed through RNA-seq studies. Our study identifies alterations in the AS patterns of approximately 900 genes such as tumor suppressor genes, transcription factors, splicing factors, and kinases. These findings allowed the identification of unique gene signatures for which AS is misregulated in both Epstein-Barr virus-associated gastric cancer and EBV-negative gastric cancer. Moreover, we show that the expression of Epstein-Barr nuclear antigen 1 (EBNA1) leads to modifications in the AS profile of cellular genes and that the EBNA1 protein interacts with cellular splicing factors. These findings provide insights into the molecular differences between various types of gastric cancer and suggest a role for the EBNA1 protein in the dysregulation of cellular AS.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0176880&type=printable |
| spellingShingle | Victoria E S Armero Marie-Pier Tremblay Andréa Allaire Simon Boudreault Camille Martenon-Brodeur Cyntia Duval Mathieu Durand Elvy Lapointe Philippe Thibault Maude Tremblay-Létourneau Jean-Pierre Perreault Michelle S Scott Martin Bisaillon Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. PLoS ONE |
| title | Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. |
| title_full | Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. |
| title_fullStr | Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. |
| title_full_unstemmed | Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. |
| title_short | Transcriptome-wide analysis of alternative RNA splicing events in Epstein-Barr virus-associated gastric carcinomas. |
| title_sort | transcriptome wide analysis of alternative rna splicing events in epstein barr virus associated gastric carcinomas |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0176880&type=printable |
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