miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas
Abstract Gliomas are the predominant cause of cancer-related deaths among the young population. Even after incorporation of IDH1/2 mutations and 1p19q codeletion there are doubts regarding adjuvant treatment in WHO G2/G3 gliomas. miRNA molecules control about 30% of all genes, also many oncogenes, t...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-80656-z |
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| author | Mateusz Bilski Marzanna Ciesielka Magdalena Orzechowska Bożena Jarosz Paulina Całka Sylwia Bilska Agata Banach Gabriela Czaja Jacek Fijuth Łukasz Kuncman |
| author_facet | Mateusz Bilski Marzanna Ciesielka Magdalena Orzechowska Bożena Jarosz Paulina Całka Sylwia Bilska Agata Banach Gabriela Czaja Jacek Fijuth Łukasz Kuncman |
| author_sort | Mateusz Bilski |
| collection | DOAJ |
| description | Abstract Gliomas are the predominant cause of cancer-related deaths among the young population. Even after incorporation of IDH1/2 mutations and 1p19q codeletion there are doubts regarding adjuvant treatment in WHO G2/G3 gliomas. miRNA molecules control about 30% of all genes, also many oncogenes, tumor suppressor genes and genes responsible for the response to ionizing radiation and systemic treatment. Patients with brain gliomas exhibit miRNA disorders. We aimed to evaluate the expression of miR-200 family members in relation to selected clinico-pathological factors and their prognostic value. We enrolled 53 patients diagnosed with WHO G2/G3 brain gliomas treated between 2012–2016. RT-qPCR based expression of miR-200 family was assessed in tumor and surrounding non-cancerous tissue. An analysis of selected clinico-pathological features was carried out. A logistic regression model was prepared for the miRNA signature. The predictive potential of the signature was assessed using the ROC curve. A stepwise backward regression model was used to select variables with a significant predictive potential related to OS. It was shown that miR-200a-3p, miR-200a-5p, miR-200c-5p, miR-141-3p and miR-429 can be independent predictors of survival. Better 2- and 5-year OS was associated with higher expression of miR-200a-3p, miR141-3p and lower expression of miR-200a-5p, miR-200c-5p, miR-429. The strongest predictors of survival were miR-200a-5p, miR-200b-3p, miR-200c-5p, miR-141-3p, miR-429, tumor volume and CTV. Members of the miR-200 family exhibit prognostic value for 2- and 5-year OS. Presented predictive models of survival may be clinically useful for treatment optimization. |
| format | Article |
| id | doaj-art-b7f3a899db824a89aa852940bab59e8d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-b7f3a899db824a89aa852940bab59e8d2024-12-01T12:19:38ZengNature PortfolioScientific Reports2045-23222024-11-0114111310.1038/s41598-024-80656-zmiR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomasMateusz Bilski0Marzanna Ciesielka1Magdalena Orzechowska2Bożena Jarosz3Paulina Całka4Sylwia Bilska5Agata Banach6Gabriela Czaja7Jacek Fijuth8Łukasz Kuncman9Department of Radiotherapy, Medical University of LublinChair and Department of Forensic Medicine, Medical University of LublinDepartment of Molecular Carcinogenesis, Medical University of ŁódźChair and Department of Neurosurgery and Paediatric Neurosurgery, Medical University of LublinChair and Department of Forensic Medicine, Medical University of LublinClinical Genetics Center, St. John’s Cancer CenterChair and Department of Neurosurgery and Paediatric Neurosurgery, Medical University of LublinChair and Department of Neurosurgery and Paediatric Neurosurgery, Medical University of LublinDepartment of Radiotherapy, Medical University of ŁódźDepartment of Radiotherapy, Medical University of ŁódźAbstract Gliomas are the predominant cause of cancer-related deaths among the young population. Even after incorporation of IDH1/2 mutations and 1p19q codeletion there are doubts regarding adjuvant treatment in WHO G2/G3 gliomas. miRNA molecules control about 30% of all genes, also many oncogenes, tumor suppressor genes and genes responsible for the response to ionizing radiation and systemic treatment. Patients with brain gliomas exhibit miRNA disorders. We aimed to evaluate the expression of miR-200 family members in relation to selected clinico-pathological factors and their prognostic value. We enrolled 53 patients diagnosed with WHO G2/G3 brain gliomas treated between 2012–2016. RT-qPCR based expression of miR-200 family was assessed in tumor and surrounding non-cancerous tissue. An analysis of selected clinico-pathological features was carried out. A logistic regression model was prepared for the miRNA signature. The predictive potential of the signature was assessed using the ROC curve. A stepwise backward regression model was used to select variables with a significant predictive potential related to OS. It was shown that miR-200a-3p, miR-200a-5p, miR-200c-5p, miR-141-3p and miR-429 can be independent predictors of survival. Better 2- and 5-year OS was associated with higher expression of miR-200a-3p, miR141-3p and lower expression of miR-200a-5p, miR-200c-5p, miR-429. The strongest predictors of survival were miR-200a-5p, miR-200b-3p, miR-200c-5p, miR-141-3p, miR-429, tumor volume and CTV. Members of the miR-200 family exhibit prognostic value for 2- and 5-year OS. Presented predictive models of survival may be clinically useful for treatment optimization.https://doi.org/10.1038/s41598-024-80656-zGliomaMiRNASurvivalBrain tumorPrognostic factor |
| spellingShingle | Mateusz Bilski Marzanna Ciesielka Magdalena Orzechowska Bożena Jarosz Paulina Całka Sylwia Bilska Agata Banach Gabriela Czaja Jacek Fijuth Łukasz Kuncman miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas Scientific Reports Glioma MiRNA Survival Brain tumor Prognostic factor |
| title | miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas |
| title_full | miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas |
| title_fullStr | miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas |
| title_full_unstemmed | miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas |
| title_short | miR-200 family as new potential prognostic factor of overall survival of patients with WHO G2 and WHO G3 brain gliomas |
| title_sort | mir 200 family as new potential prognostic factor of overall survival of patients with who g2 and who g3 brain gliomas |
| topic | Glioma MiRNA Survival Brain tumor Prognostic factor |
| url | https://doi.org/10.1038/s41598-024-80656-z |
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