Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite
Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined w...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642400440X |
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| author | Hye Yeong Lee Seong Bae An Sae Yeon Hwang Gwang Yong Hwang Hye-Lan Lee Hyun Jung Park Joongkyum Shin Keung Nyun Kim Sung Won Wee Sol Lip Yoon Yoon Ha |
| author_facet | Hye Yeong Lee Seong Bae An Sae Yeon Hwang Gwang Yong Hwang Hye-Lan Lee Hyun Jung Park Joongkyum Shin Keung Nyun Kim Sung Won Wee Sol Lip Yoon Yoon Ha |
| author_sort | Hye Yeong Lee |
| collection | DOAJ |
| description | Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined with stromal vascular fraction (SVF) and low-dose recombinant human BMP-2 (rhBMP-2) to enhance osteodifferentiation and angiogenesis. Through a series of in vitro studies and preclinical models involving rats and minipigs, we demonstrated that the hydrogel system enables the sustained release of rhBMP-2, resulting in significantly improved bone density and integration, alongside reduced inflammatory responses. The combination of rhBMP-2 and SVF in this injectable formulation yielded superior spinal fusion outcomes, with enhanced mechanical properties and increased bone mass in both small and large animal models. These findings suggest that this strategy offers a promising and safer alternative for spinal fusion, with strong potential for clinical application. |
| format | Article |
| id | doaj-art-b7df3cc916fc42e2829351f1f2ea73a9 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-b7df3cc916fc42e2829351f1f2ea73a92025-01-17T04:52:01ZengElsevierMaterials Today Bio2590-00642025-02-0130101379Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel compositeHye Yeong Lee0Seong Bae An1Sae Yeon Hwang2Gwang Yong Hwang3Hye-Lan Lee4Hyun Jung Park5Joongkyum Shin6Keung Nyun Kim7Sung Won Wee8Sol Lip Yoon9Yoon Ha10Spine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaDepartment of Neurosurgery, School of Medicine, CHA University, CHA Bundang Medical Center, Seongnam-si, 13496, Gyeonggi-do, Republic of Korea; Graduate School, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaDepartment of Research Center, CGBio., co. Ltd, Seongnam-si, Gyeonggi-do, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of KoreaSpine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of Korea; POSTECH Biotech Center, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, Republic of Korea; Corresponding author. Spine & Spinal Cord Institute, Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, 03722, Republic of Korea.Spinal fusion surgery remains a significant challenge due to limitations in current bone graft materials, particularly in terms of bioactivity, integration, and safety. This study presents an innovative approach using an injectable hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) hydrogel combined with stromal vascular fraction (SVF) and low-dose recombinant human BMP-2 (rhBMP-2) to enhance osteodifferentiation and angiogenesis. Through a series of in vitro studies and preclinical models involving rats and minipigs, we demonstrated that the hydrogel system enables the sustained release of rhBMP-2, resulting in significantly improved bone density and integration, alongside reduced inflammatory responses. The combination of rhBMP-2 and SVF in this injectable formulation yielded superior spinal fusion outcomes, with enhanced mechanical properties and increased bone mass in both small and large animal models. These findings suggest that this strategy offers a promising and safer alternative for spinal fusion, with strong potential for clinical application.http://www.sciencedirect.com/science/article/pii/S259000642400440XInjectable puttyStromal vascular fractionLow-dose recombinant human BMP-2Osteodifferentiation and angiogenesisSynergySpinal fusion surgery |
| spellingShingle | Hye Yeong Lee Seong Bae An Sae Yeon Hwang Gwang Yong Hwang Hye-Lan Lee Hyun Jung Park Joongkyum Shin Keung Nyun Kim Sung Won Wee Sol Lip Yoon Yoon Ha Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite Materials Today Bio Injectable putty Stromal vascular fraction Low-dose recombinant human BMP-2 Osteodifferentiation and angiogenesis Synergy Spinal fusion surgery |
| title | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
| title_full | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
| title_fullStr | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
| title_full_unstemmed | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
| title_short | Synergistic enhancement of spinal fusion in preclinical models using low-dose rhBMP-2 and stromal vascular fraction in an injectable hydrogel composite |
| title_sort | synergistic enhancement of spinal fusion in preclinical models using low dose rhbmp 2 and stromal vascular fraction in an injectable hydrogel composite |
| topic | Injectable putty Stromal vascular fraction Low-dose recombinant human BMP-2 Osteodifferentiation and angiogenesis Synergy Spinal fusion surgery |
| url | http://www.sciencedirect.com/science/article/pii/S259000642400440X |
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