Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase
α-amylase can effectively inhibit the activity of digestive enzymes and alter nutrient absorption. The impact of ovum hydrolysates of sea cucumbers on α-amylase activity was investigated in this study. The protein hydrolysates generated using different proteases (pepsin, trypsin, and neutral proteas...
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2024-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/24/4119 |
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| author | Xu Yan Fengjiao Fan Zijin Qin Lijuan Zhang Shuang Guan Shiying Han Xiufang Dong Hui Chen Zhe Xu Tingting Li |
| author_facet | Xu Yan Fengjiao Fan Zijin Qin Lijuan Zhang Shuang Guan Shiying Han Xiufang Dong Hui Chen Zhe Xu Tingting Li |
| author_sort | Xu Yan |
| collection | DOAJ |
| description | α-amylase can effectively inhibit the activity of digestive enzymes and alter nutrient absorption. The impact of ovum hydrolysates of sea cucumbers on α-amylase activity was investigated in this study. The protein hydrolysates generated using different proteases (pepsin, trypsin, and neutral protease) and molecular weights (less than 3000 and more than 3000) were investigated. The results showed that all three different hydrolysates demonstrated calcium-chelating activity and induced a fluorescence-quenching effect on α-amylase. The sea cucumber ovum hydrolysate with a molecular weight of less than 3000 Da, isolated using trypsin, showed the most effective inhibitory effect on α-amylase, with an inhibition rate of 53.9%, and the inhibition type was identified as mixed forms of inhibition. In conclusion, the generation and utilization of protein hydrolysates from sea cucumber ovum as a functional food ingredient could be a potential approach to add value to low-cost seafood by-products. |
| format | Article |
| id | doaj-art-b7b7d0c9a525467194b45f671c6c682f |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-b7b7d0c9a525467194b45f671c6c682f2024-12-27T14:26:34ZengMDPI AGFoods2304-81582024-12-011324411910.3390/foods13244119Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-AmylaseXu Yan0Fengjiao Fan1Zijin Qin2Lijuan Zhang3Shuang Guan4Shiying Han5Xiufang Dong6Hui Chen7Zhe Xu8Tingting Li9Key Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, ChinaDepartment of Food Science and Technology, The University of Georgia, Athens, GA 30602, USAKey Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, ChinaKey Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, ChinaKey Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, ChinaCollege of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, ChinaKey Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Zhejiang University of Technology, Hangzhou 310014, ChinaKey Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, ChinaKey Laboratory of Biotechnology and Bioresources Utilization, College of Life Sciences, Dalian Minzu University, Ministry of Education, Dalian 116600, Chinaα-amylase can effectively inhibit the activity of digestive enzymes and alter nutrient absorption. The impact of ovum hydrolysates of sea cucumbers on α-amylase activity was investigated in this study. The protein hydrolysates generated using different proteases (pepsin, trypsin, and neutral protease) and molecular weights (less than 3000 and more than 3000) were investigated. The results showed that all three different hydrolysates demonstrated calcium-chelating activity and induced a fluorescence-quenching effect on α-amylase. The sea cucumber ovum hydrolysate with a molecular weight of less than 3000 Da, isolated using trypsin, showed the most effective inhibitory effect on α-amylase, with an inhibition rate of 53.9%, and the inhibition type was identified as mixed forms of inhibition. In conclusion, the generation and utilization of protein hydrolysates from sea cucumber ovum as a functional food ingredient could be a potential approach to add value to low-cost seafood by-products.https://www.mdpi.com/2304-8158/13/24/4119sea cucumber ovumhydrolysateα-amylaseinhibitory activitycalcium chelation |
| spellingShingle | Xu Yan Fengjiao Fan Zijin Qin Lijuan Zhang Shuang Guan Shiying Han Xiufang Dong Hui Chen Zhe Xu Tingting Li Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase Foods sea cucumber ovum hydrolysate α-amylase inhibitory activity calcium chelation |
| title | Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase |
| title_full | Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase |
| title_fullStr | Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase |
| title_full_unstemmed | Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase |
| title_short | Preparation and Characterization of Calcium-Chelated Sea Cucumber Ovum Hydrolysate and the Inhibitory Effect on α-Amylase |
| title_sort | preparation and characterization of calcium chelated sea cucumber ovum hydrolysate and the inhibitory effect on α amylase |
| topic | sea cucumber ovum hydrolysate α-amylase inhibitory activity calcium chelation |
| url | https://www.mdpi.com/2304-8158/13/24/4119 |
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