Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis
Abstract Background Cardiac fibrosis plays a critical role in the progression of various forms of heart disease, significantly increasing the risk of sudden cardiac death. However, currently, there are no therapeutic strategies available to prevent the onset of cardiac fibrosis. Methods and results...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-03083-2 |
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| author | Xueli Zhao Yuze Qin Bowen Li Yue Wang Jiao Liu Bo Wang Jia Zhao Jiaqi Yin Lanlan Zhang Jing Li Junzhe Huang Kun Chen Liwen Liu Yuanming Wu |
| author_facet | Xueli Zhao Yuze Qin Bowen Li Yue Wang Jiao Liu Bo Wang Jia Zhao Jiaqi Yin Lanlan Zhang Jing Li Junzhe Huang Kun Chen Liwen Liu Yuanming Wu |
| author_sort | Xueli Zhao |
| collection | DOAJ |
| description | Abstract Background Cardiac fibrosis plays a critical role in the progression of various forms of heart disease, significantly increasing the risk of sudden cardiac death. However, currently, there are no therapeutic strategies available to prevent the onset of cardiac fibrosis. Methods and results Here, biomimetic ATP-responsive nanozymes based on genetically engineered cell membranes are adapted to specifically recognize activated cardiac fibroblasts (CFs) for the treatment of cardiac fibrosis. By fusing the anti-FAP CAR genetically engineered cell membrane to zeolitic imidazole frameworks-90 (zif-90) cores loaded with antioxidant nanozymes CeO2 and siCTGF (siRNA targeting CTGF), these nanoparticles, called FM@zif-90/Ce/siR NPs, are demonstrated to effectively reduce the accumulation of myofibroblasts and the formation of fibrotic tissue, while restoring cardiac function. Conclusions These findings demonstrate that the combination of CeO2 and siCTGF has a beneficial curative effect on cardiac fibrosis, with significant translational potential. |
| format | Article |
| id | doaj-art-b73ed9b826474456a10be1c3030a2321 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-b73ed9b826474456a10be1c3030a23212025-01-12T12:38:18ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123111810.1186/s12951-024-03083-2Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosisXueli Zhao0Yuze Qin1Bowen Li2Yue Wang3Jiao Liu4Bo Wang5Jia Zhao6Jiaqi Yin7Lanlan Zhang8Jing Li9Junzhe Huang10Kun Chen11Liwen Liu12Yuanming Wu13Department of Biochemistry and Molecular Biology, Shaanxi Provincial Key Laboratory of Clinical Genetics, School of Basic Medicine, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Biochemistry and Molecular Biology, Shaanxi Provincial Key Laboratory of Clinical Genetics, School of Basic Medicine, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Anatomy, Histology and Embryology and K.K. Leung Brain Research Centre, School of Basic Medicine, Fourth Military Medical UniversityDepartment of Ultrasound, Xijing Hypertrophic Cardiomyopathy Center, Xijing Hospital, Fourth Military Medical UniversityDepartment of Laboratory, Tangdu Hospital, Fourth Military Medical UniversityAbstract Background Cardiac fibrosis plays a critical role in the progression of various forms of heart disease, significantly increasing the risk of sudden cardiac death. However, currently, there are no therapeutic strategies available to prevent the onset of cardiac fibrosis. Methods and results Here, biomimetic ATP-responsive nanozymes based on genetically engineered cell membranes are adapted to specifically recognize activated cardiac fibroblasts (CFs) for the treatment of cardiac fibrosis. By fusing the anti-FAP CAR genetically engineered cell membrane to zeolitic imidazole frameworks-90 (zif-90) cores loaded with antioxidant nanozymes CeO2 and siCTGF (siRNA targeting CTGF), these nanoparticles, called FM@zif-90/Ce/siR NPs, are demonstrated to effectively reduce the accumulation of myofibroblasts and the formation of fibrotic tissue, while restoring cardiac function. Conclusions These findings demonstrate that the combination of CeO2 and siCTGF has a beneficial curative effect on cardiac fibrosis, with significant translational potential.https://doi.org/10.1186/s12951-024-03083-2Cardiac fibrosisZeolitic imidazole frameworks-90NanozymeATP-responsiveGenetically engineered |
| spellingShingle | Xueli Zhao Yuze Qin Bowen Li Yue Wang Jiao Liu Bo Wang Jia Zhao Jiaqi Yin Lanlan Zhang Jing Li Junzhe Huang Kun Chen Liwen Liu Yuanming Wu Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis Journal of Nanobiotechnology Cardiac fibrosis Zeolitic imidazole frameworks-90 Nanozyme ATP-responsive Genetically engineered |
| title | Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis |
| title_full | Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis |
| title_fullStr | Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis |
| title_full_unstemmed | Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis |
| title_short | Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis |
| title_sort | genetically engineered biomimetic atp responsive nanozyme for the treatment of cardiac fibrosis |
| topic | Cardiac fibrosis Zeolitic imidazole frameworks-90 Nanozyme ATP-responsive Genetically engineered |
| url | https://doi.org/10.1186/s12951-024-03083-2 |
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