Dietary Hyaluronic Acid Migrates into the Skin of Rats

Hyaluronic acid is a constituent of the skin and helps to maintain hydration. The oral intake of hyaluronic acid increases water in the horny layer as demonstrated by human trials, but in vivo kinetics has not been shown. This study confirmed the absorption, migration, and excretion of 14C-labeled h...

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Main Authors: Mariko Oe, Koichi Mitsugi, Wataru Odanaka, Hideto Yoshida, Ryosuke Matsuoka, Satoshi Seino, Tomoyuki Kanemitsu, Yasunobu Masuda
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/378024
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author Mariko Oe
Koichi Mitsugi
Wataru Odanaka
Hideto Yoshida
Ryosuke Matsuoka
Satoshi Seino
Tomoyuki Kanemitsu
Yasunobu Masuda
author_facet Mariko Oe
Koichi Mitsugi
Wataru Odanaka
Hideto Yoshida
Ryosuke Matsuoka
Satoshi Seino
Tomoyuki Kanemitsu
Yasunobu Masuda
author_sort Mariko Oe
collection DOAJ
description Hyaluronic acid is a constituent of the skin and helps to maintain hydration. The oral intake of hyaluronic acid increases water in the horny layer as demonstrated by human trials, but in vivo kinetics has not been shown. This study confirmed the absorption, migration, and excretion of 14C-labeled hyaluronic acid (14C-hyaluronic acid). 14C-hyaluronic acid was orally or intravenously administered to male SD rats aged 7 to 8 weeks. Plasma radioactivity after oral administration showed the highest level 8 hours after administration, and orally administered 14C-hyaluronic acid was found in the blood. Approximately 90% of 14C-hyaluronic acid was absorbed from the digestive tract and used as an energy source or a structural constituent of tissues based on tests of the urine, feces, expired air, and cadaver up to 168 hours (one week) after administration. The autoradiographic results suggested that radioactivity was distributed systematically and then reduced over time. The radioactivity was higher in the skin than in the blood at 24 and 96 hours after administration. The results show the possibility that orally administered hyaluronic acid migrated into the skin. No excessive accumulation was observed and more than 90% of the hyaluronic acid was excreted in expired air or urine.
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language English
publishDate 2014-01-01
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spelling doaj-art-b7225f7a7ce04d80a534d9ce39e7f97c2025-08-20T03:39:28ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/378024378024Dietary Hyaluronic Acid Migrates into the Skin of RatsMariko Oe0Koichi Mitsugi1Wataru Odanaka2Hideto Yoshida3Ryosuke Matsuoka4Satoshi Seino5Tomoyuki Kanemitsu6Yasunobu Masuda7R&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanADME & Tox. Research Institute, Sekisui Medical Co., Ltd., 2117 Muramatsu, Tokai-mura, Naka-gun, Ibaraki 319-1182, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanR&D Division, Kewpie Corporation, 2-5-7 Sengawa Kewport, Sengawa, Chofu, Tokyo 182-0002, JapanHyaluronic acid is a constituent of the skin and helps to maintain hydration. The oral intake of hyaluronic acid increases water in the horny layer as demonstrated by human trials, but in vivo kinetics has not been shown. This study confirmed the absorption, migration, and excretion of 14C-labeled hyaluronic acid (14C-hyaluronic acid). 14C-hyaluronic acid was orally or intravenously administered to male SD rats aged 7 to 8 weeks. Plasma radioactivity after oral administration showed the highest level 8 hours after administration, and orally administered 14C-hyaluronic acid was found in the blood. Approximately 90% of 14C-hyaluronic acid was absorbed from the digestive tract and used as an energy source or a structural constituent of tissues based on tests of the urine, feces, expired air, and cadaver up to 168 hours (one week) after administration. The autoradiographic results suggested that radioactivity was distributed systematically and then reduced over time. The radioactivity was higher in the skin than in the blood at 24 and 96 hours after administration. The results show the possibility that orally administered hyaluronic acid migrated into the skin. No excessive accumulation was observed and more than 90% of the hyaluronic acid was excreted in expired air or urine.http://dx.doi.org/10.1155/2014/378024
spellingShingle Mariko Oe
Koichi Mitsugi
Wataru Odanaka
Hideto Yoshida
Ryosuke Matsuoka
Satoshi Seino
Tomoyuki Kanemitsu
Yasunobu Masuda
Dietary Hyaluronic Acid Migrates into the Skin of Rats
The Scientific World Journal
title Dietary Hyaluronic Acid Migrates into the Skin of Rats
title_full Dietary Hyaluronic Acid Migrates into the Skin of Rats
title_fullStr Dietary Hyaluronic Acid Migrates into the Skin of Rats
title_full_unstemmed Dietary Hyaluronic Acid Migrates into the Skin of Rats
title_short Dietary Hyaluronic Acid Migrates into the Skin of Rats
title_sort dietary hyaluronic acid migrates into the skin of rats
url http://dx.doi.org/10.1155/2014/378024
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