Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis
Alzheimer’s disease (AD) is a complex neurodegenerative disorder, with amyloid-beta (Aβ) aggregation playing a key role in its pathogenesis. Aβ-induced oxidative stress leads to neuronal damage, mitochondrial dysfunction, and apoptosis, making antioxidative strategies promising for AD treatment. Thi...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Aging Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1515092/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556742750601216 |
|---|---|
| author | Jiaxuan He Peiye Xu Ting Xu Haiyang Yu Lei Wang Rongbing Chen Kun Zhang Yueliang Yao Yanyan Xie Qinsi Yang Wei Wu Da Sun Dejun Wu |
| author_facet | Jiaxuan He Peiye Xu Ting Xu Haiyang Yu Lei Wang Rongbing Chen Kun Zhang Yueliang Yao Yanyan Xie Qinsi Yang Wei Wu Da Sun Dejun Wu |
| author_sort | Jiaxuan He |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a complex neurodegenerative disorder, with amyloid-beta (Aβ) aggregation playing a key role in its pathogenesis. Aβ-induced oxidative stress leads to neuronal damage, mitochondrial dysfunction, and apoptosis, making antioxidative strategies promising for AD treatment. This study investigates the effects of hydrogen-rich water (HRW) in a zebrafish AD model. Zebrafish were exposed to aluminum chloride to induce AD-like pathology and then treated with HRW using a nanobubble device. Behavioral assays, ELISA, Hematoxylin–eosin (H&E) staining, and reactive oxygen species (ROS) and neutrophil fluorescence labeling were employed to assess HRW’s impact. Additionally, 16S rRNA sequencing analyzed HRW’s effect on gut microbiota. HRW can significantly improve cognitive impairment and depression-like behavior in zebrafish AD model, reduce Aβ deposition (p < 0.0001), regulate liver Soluble epoxide hydrolase (sEH) levels (p < 0.05), reduce neuroinflammation, and reduce oxidative stress. Furthermore, HRW reduced the number of harmful bacteria linked to AD pathology by restoring the balance of microbiota in the gut. These findings suggest that HRW has potential as a therapeutic strategy for AD by targeting oxidative stress, inflammation, and gut-brain axis modulation. |
| format | Article |
| id | doaj-art-b6fc98a8d6b849c2bdf6264b20177fc3 |
| institution | Kabale University |
| issn | 1663-4365 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj-art-b6fc98a8d6b849c2bdf6264b20177fc32025-01-07T06:40:55ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652025-01-011610.3389/fnagi.2024.15150921515092Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axisJiaxuan He0Peiye Xu1Ting Xu2Haiyang Yu3Lei Wang4Rongbing Chen5Kun Zhang6Yueliang Yao7Yanyan Xie8Qinsi Yang9Wei Wu10Da Sun11Dejun Wu12Institute of Life Sciences and Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou, ChinaInstitute of Life Sciences and Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou, ChinaInstitute of Life Sciences and Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou, ChinaInstitute of Life Sciences and Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou, ChinaTechnical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, ChinaDepartment of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaChongqing Municipality Clinical Research Center for Geriatric Diseases, Chongqing University Three Gorges Hospital, Chongqing, ChinaFuzhou Innovation Center for AI Drug, Fuzhou Medical College of Nanchang University, Fuzhou, ChinaThe Affiliated Kangning Hospital of Wenzhou Medical University, Wenzhou, ChinaWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, ChinaKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing, ChinaInstitute of Life Sciences and Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou, ChinaDepartment of Geriatric Medicine, Quzhou People’s Hospital, Quzhou, ChinaAlzheimer’s disease (AD) is a complex neurodegenerative disorder, with amyloid-beta (Aβ) aggregation playing a key role in its pathogenesis. Aβ-induced oxidative stress leads to neuronal damage, mitochondrial dysfunction, and apoptosis, making antioxidative strategies promising for AD treatment. This study investigates the effects of hydrogen-rich water (HRW) in a zebrafish AD model. Zebrafish were exposed to aluminum chloride to induce AD-like pathology and then treated with HRW using a nanobubble device. Behavioral assays, ELISA, Hematoxylin–eosin (H&E) staining, and reactive oxygen species (ROS) and neutrophil fluorescence labeling were employed to assess HRW’s impact. Additionally, 16S rRNA sequencing analyzed HRW’s effect on gut microbiota. HRW can significantly improve cognitive impairment and depression-like behavior in zebrafish AD model, reduce Aβ deposition (p < 0.0001), regulate liver Soluble epoxide hydrolase (sEH) levels (p < 0.05), reduce neuroinflammation, and reduce oxidative stress. Furthermore, HRW reduced the number of harmful bacteria linked to AD pathology by restoring the balance of microbiota in the gut. These findings suggest that HRW has potential as a therapeutic strategy for AD by targeting oxidative stress, inflammation, and gut-brain axis modulation.https://www.frontiersin.org/articles/10.3389/fnagi.2024.1515092/fullAlzheimer’s diseasezebrafishhydrogen-rich waterbehaviorantioxidantreduced Aβ deposition |
| spellingShingle | Jiaxuan He Peiye Xu Ting Xu Haiyang Yu Lei Wang Rongbing Chen Kun Zhang Yueliang Yao Yanyan Xie Qinsi Yang Wei Wu Da Sun Dejun Wu Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis Frontiers in Aging Neuroscience Alzheimer’s disease zebrafish hydrogen-rich water behavior antioxidant reduced Aβ deposition |
| title | Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis |
| title_full | Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis |
| title_fullStr | Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis |
| title_full_unstemmed | Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis |
| title_short | Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis |
| title_sort | therapeutic potential of hydrogen rich water in zebrafish model of alzheimer s disease targeting oxidative stress inflammation and the gut brain axis |
| topic | Alzheimer’s disease zebrafish hydrogen-rich water behavior antioxidant reduced Aβ deposition |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1515092/full |
| work_keys_str_mv | AT jiaxuanhe therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT peiyexu therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT tingxu therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT haiyangyu therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT leiwang therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT rongbingchen therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT kunzhang therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT yueliangyao therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT yanyanxie therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT qinsiyang therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT weiwu therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT dasun therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis AT dejunwu therapeuticpotentialofhydrogenrichwaterinzebrafishmodelofalzheimersdiseasetargetingoxidativestressinflammationandthegutbrainaxis |