The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors
ABSTRACT In bacteria, if a ribosome translates an mRNA lacking a stop codon it becomes stalled at the 3′ end of the message. These ribosomes must be rescued by trans-translation or the alternative rescue factors (ArfA or ArfB). However, mounting evidence suggests that the ribosome quality control (R...
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American Society for Microbiology
2024-12-01
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| Series: | mBio |
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.02322-24 |
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| author | Katrina Callan Cassidy R. Prince Heather A. Feaga |
| author_facet | Katrina Callan Cassidy R. Prince Heather A. Feaga |
| author_sort | Katrina Callan |
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| description | ABSTRACT In bacteria, if a ribosome translates an mRNA lacking a stop codon it becomes stalled at the 3′ end of the message. These ribosomes must be rescued by trans-translation or the alternative rescue factors (ArfA or ArfB). However, mounting evidence suggests that the ribosome quality control (RQC) pathway may also rescue non-stop ribosomes. Here, we surveyed the conservation of ribosome rescue pathways in >15,000 bacterial genomes. We found that trans-translation is conserved in >97% of bacterial genomes, while the other rescue pathways are restricted to particular phyla. We did not detect the gene encoding RqcH, the major mediator of RQC, in Proteobacteria (Pseudomonadota). In all Proteobacteria investigated to date, trans-translation is essential in the absence of the Arf proteins. Therefore, we tested whether expression of RQC components from Bacillus subtilis could rescue viability in the absence of trans-translation and ArfA in Escherichia coli. We found that the RQC pathway indeed functions in E. coli and rescues the well-documented synthetic lethal phenotype of ∆ssrA∆arfA. Moreover, we show that the RQC pathway in B. subtilis is essential in the absence of trans-translation and ArfA, further supporting a role for the RQC pathway in the rescue of non-stop ribosomes. Finally, we report a strong co-occurrence between RqcH and the ribosome splitting factor MutS2, but present experimental evidence that there are likely additional ribosome splitting factors beyond MutS2 in B. subtilis. Altogether, our work supports a role for RQC in non-stop ribosome rescue and provides a broad survey of ribosome rescue pathways in diverse bacteria.IMPORTANCEIn bacteria, it is estimated that 2%–4% of all translation reactions terminate with the ribosome stalled on a damaged mRNA lacking a stop codon. Mechanisms that rescue these ribosomes are essential for viability. We determined the functional overlap between the ribosome quality control pathway and the classical non-stop rescue systems [alternative rescue factor (ArfA) and trans-translation] in a representative Firmicute and Proteobacterium, phyla that are evolutionarily distinct. Furthermore, we used a bioinformatics approach to examine the conservation and overlap of various ribosome rescue systems in >15,000 species throughout the bacterial domain. These results provide key insights into ribosome rescue in diverse phyla. |
| format | Article |
| id | doaj-art-b5ec26fc4a3c48d6af0caad0fd6a78f1 |
| institution | Kabale University |
| issn | 2150-7511 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj-art-b5ec26fc4a3c48d6af0caad0fd6a78f12024-12-11T14:02:31ZengAmerican Society for MicrobiologymBio2150-75112024-12-01151210.1128/mbio.02322-24The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factorsKatrina Callan0Cassidy R. Prince1Heather A. Feaga2Department of Microbiology, Cornell University, Ithaca, New York, USADepartment of Microbiology, Cornell University, Ithaca, New York, USADepartment of Microbiology, Cornell University, Ithaca, New York, USAABSTRACT In bacteria, if a ribosome translates an mRNA lacking a stop codon it becomes stalled at the 3′ end of the message. These ribosomes must be rescued by trans-translation or the alternative rescue factors (ArfA or ArfB). However, mounting evidence suggests that the ribosome quality control (RQC) pathway may also rescue non-stop ribosomes. Here, we surveyed the conservation of ribosome rescue pathways in >15,000 bacterial genomes. We found that trans-translation is conserved in >97% of bacterial genomes, while the other rescue pathways are restricted to particular phyla. We did not detect the gene encoding RqcH, the major mediator of RQC, in Proteobacteria (Pseudomonadota). In all Proteobacteria investigated to date, trans-translation is essential in the absence of the Arf proteins. Therefore, we tested whether expression of RQC components from Bacillus subtilis could rescue viability in the absence of trans-translation and ArfA in Escherichia coli. We found that the RQC pathway indeed functions in E. coli and rescues the well-documented synthetic lethal phenotype of ∆ssrA∆arfA. Moreover, we show that the RQC pathway in B. subtilis is essential in the absence of trans-translation and ArfA, further supporting a role for the RQC pathway in the rescue of non-stop ribosomes. Finally, we report a strong co-occurrence between RqcH and the ribosome splitting factor MutS2, but present experimental evidence that there are likely additional ribosome splitting factors beyond MutS2 in B. subtilis. Altogether, our work supports a role for RQC in non-stop ribosome rescue and provides a broad survey of ribosome rescue pathways in diverse bacteria.IMPORTANCEIn bacteria, it is estimated that 2%–4% of all translation reactions terminate with the ribosome stalled on a damaged mRNA lacking a stop codon. Mechanisms that rescue these ribosomes are essential for viability. We determined the functional overlap between the ribosome quality control pathway and the classical non-stop rescue systems [alternative rescue factor (ArfA) and trans-translation] in a representative Firmicute and Proteobacterium, phyla that are evolutionarily distinct. Furthermore, we used a bioinformatics approach to examine the conservation and overlap of various ribosome rescue systems in >15,000 species throughout the bacterial domain. These results provide key insights into ribosome rescue in diverse phyla.https://journals.asm.org/doi/10.1128/mbio.02322-24ribosomestranslationtrans-translationbacteriamolecular geneticsRNA binding proteins |
| spellingShingle | Katrina Callan Cassidy R. Prince Heather A. Feaga The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors mBio ribosomes translation trans-translation bacteria molecular genetics RNA binding proteins |
| title | The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors |
| title_full | The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors |
| title_fullStr | The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors |
| title_full_unstemmed | The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors |
| title_short | The ribosome-associated quality control pathway supports survival in the absence of non-stop ribosome rescue factors |
| title_sort | ribosome associated quality control pathway supports survival in the absence of non stop ribosome rescue factors |
| topic | ribosomes translation trans-translation bacteria molecular genetics RNA binding proteins |
| url | https://journals.asm.org/doi/10.1128/mbio.02322-24 |
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