Expression of lncRNA CDKN2BAS in oral squamous cell carcinoma and its significance on cell proliferation and invasion
[Objective:] To investigate the expression intensity of lncRNA CDKN2BAS in oral squamous cell carcinoma (OSCC) tissues, and to determine whether the expression level is related to tumor proliferation and invasion. [Methods:] A total of 96 cases of patients with OSCC treated in our hospital from Marc...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-08-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.004 |
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| Summary: | [Objective:] To investigate the expression intensity of lncRNA CDKN2BAS in oral squamous cell carcinoma (OSCC) tissues, and to determine whether the expression level is related to tumor proliferation and invasion. [Methods:] A total of 96 cases of patients with OSCC treated in our hospital from March 2016 to March 2021 were selected as the research objects. The CAL‐27 cells were cultured and divided into siRNA group, group B and group C, and transfected by using transfection reagent with the small molecule interfering sequence of CDKN2BAS (group siRNA), control sequence (group C) and transfection reagent only (group B), respectively. The real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to detect the expressions of CDKN2BAS in OSCC tissues and cells; the MTT experiment and plate clone formation experiment were usedto detect cell proliferation activity, and the transwell experiment was used to detect cell migration and invasion. [Results:] Theexpression level of CDKN2BAS in OSCC tissue (2.61±0.21) was significantly higher than that in the control group (1.03±0.11) (P<0.001). The expression level of CDKN2BAS in poorly differentiated tissues was higher than that in medium andhigh differentiation, the expression level of CDKN2BAS in tissues of TNM stage Ⅲ‐Ⅳ was higher than that of stage Ⅰ‐Ⅱ, the expression level of CDKN2BAS in tissues with lymph node metastasis was higher than that in tissues without lymph node metastasis, and the expression level of CDKN2BAS in tissues with vascular involvement was higher than that without vascular recidivism, the differences were statistically significant (P<0.05). The expression level of CDKN2BAS of the cells in the siRNA group was (0.18±0.04), which was significantly lower than those in the group C (1.02±0.08) and group B (1.03±0.10) (P<0.001). The cell absorbance values in the siRNA group at 24, 48, 72 and 96 h were lower than those in the group C and group B (P<0.05). The number of cell clone, migration and invasion in siRNA group were lower than those in the group C and group B (P<0.001). [Conclusion:] This result suggests that the expression level of CDKN2BAS in OSCC tissues is increased. Silencing the expression of CDKN2BAS in CAL‐27 cells can reduce cell proliferation activity and the number of cell migration and invasion can be decreased. |
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| ISSN: | 1005-4979 |