Recent advances in single fluorescent probes for monitoring dual organelles in two channels

Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of the organelle networks are associated with multiple pathological processes. However, clearly and intuitively v...

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Main Authors: Xiu‐Zhi Yang, Shankun Yao, Jisong Wu, Jiajie Diao, Weijiang He, Zijian Guo, Yuncong Chen
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Smart Molecules
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Online Access:https://doi.org/10.1002/smo.20240040
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author Xiu‐Zhi Yang
Shankun Yao
Jisong Wu
Jiajie Diao
Weijiang He
Zijian Guo
Yuncong Chen
author_facet Xiu‐Zhi Yang
Shankun Yao
Jisong Wu
Jiajie Diao
Weijiang He
Zijian Guo
Yuncong Chen
author_sort Xiu‐Zhi Yang
collection DOAJ
description Abstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of the organelle networks are associated with multiple pathological processes. However, clearly and intuitively visualizing the highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due to the distinct advantages of facile, non‐invasiveness and dynamic detection, making it particularly well‐suited for applications in uncovering the mysterious veil of organelle interactions. Regrettably, the lack of ideal fluorescence agents has always been an obstacle in imaging the intricate behaviors of organelles. In this review, we provide a systematic discussion on the existing dual‐color and dual‐targetable molecular sensors used in monitoring organelle interactions, with a specific focus on their targeting strategies, imaging mechanisms and biological applications. Additionally, the current limitations and future development directions of dual‐targetable probes and dual‐emissives are briefly discussed. This review aims to provide guidance for researchers to develop more improved probes for studying organelle interactions in the biomedical field.
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institution Kabale University
issn 2751-4587
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language English
publishDate 2024-12-01
publisher Wiley
record_format Article
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spelling doaj-art-b57b8ebdf62b4d3a80cedc2a4cd782d42024-12-20T12:25:07ZengWileySmart Molecules2751-45872751-45952024-12-0124n/an/a10.1002/smo.20240040Recent advances in single fluorescent probes for monitoring dual organelles in two channelsXiu‐Zhi Yang0Shankun Yao1Jisong Wu2Jiajie Diao3Weijiang He4Zijian Guo5Yuncong Chen6State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaDepartment of Cancer Biology University of Cincinnati College of Medicine Cincinnati Ohio USAState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center Nanjing University Nanjing ChinaAbstract Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of the organelle networks are associated with multiple pathological processes. However, clearly and intuitively visualizing the highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due to the distinct advantages of facile, non‐invasiveness and dynamic detection, making it particularly well‐suited for applications in uncovering the mysterious veil of organelle interactions. Regrettably, the lack of ideal fluorescence agents has always been an obstacle in imaging the intricate behaviors of organelles. In this review, we provide a systematic discussion on the existing dual‐color and dual‐targetable molecular sensors used in monitoring organelle interactions, with a specific focus on their targeting strategies, imaging mechanisms and biological applications. Additionally, the current limitations and future development directions of dual‐targetable probes and dual‐emissives are briefly discussed. This review aims to provide guidance for researchers to develop more improved probes for studying organelle interactions in the biomedical field.https://doi.org/10.1002/smo.20240040bioimagingdual‐colordual‐targetedfluorescent probesorganelle interaction
spellingShingle Xiu‐Zhi Yang
Shankun Yao
Jisong Wu
Jiajie Diao
Weijiang He
Zijian Guo
Yuncong Chen
Recent advances in single fluorescent probes for monitoring dual organelles in two channels
Smart Molecules
bioimaging
dual‐color
dual‐targeted
fluorescent probes
organelle interaction
title Recent advances in single fluorescent probes for monitoring dual organelles in two channels
title_full Recent advances in single fluorescent probes for monitoring dual organelles in two channels
title_fullStr Recent advances in single fluorescent probes for monitoring dual organelles in two channels
title_full_unstemmed Recent advances in single fluorescent probes for monitoring dual organelles in two channels
title_short Recent advances in single fluorescent probes for monitoring dual organelles in two channels
title_sort recent advances in single fluorescent probes for monitoring dual organelles in two channels
topic bioimaging
dual‐color
dual‐targeted
fluorescent probes
organelle interaction
url https://doi.org/10.1002/smo.20240040
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AT shankunyao recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels
AT jisongwu recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels
AT jiajiediao recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels
AT weijianghe recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels
AT zijianguo recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels
AT yuncongchen recentadvancesinsinglefluorescentprobesformonitoringdualorganellesintwochannels