Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens
Phytopathology deals with a branch of biology encompassing pathogens that infect plants. Pathogenic fungi, bacteria, viruses, viroids, and phytoplasmas are notorious and hard to control; preventive measures are important for managing disease as early as possible. Age-old management practices are tim...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2025-01-01
|
| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-202501-0001_loop-mediated-isothermal-amplification-and-its-limit-of-detection-for-diagnostics-of-plant-pathogens.php |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526674722652160 |
|---|---|
| author | Govindan Muthukumar Ayyanar Kamalakannan Irudhayasamy Johnson Pachamuthu Kamaraj Iyyamperumal Muthuvel Shanmugam Varanavasiappan |
| author_facet | Govindan Muthukumar Ayyanar Kamalakannan Irudhayasamy Johnson Pachamuthu Kamaraj Iyyamperumal Muthuvel Shanmugam Varanavasiappan |
| author_sort | Govindan Muthukumar |
| collection | DOAJ |
| description | Phytopathology deals with a branch of biology encompassing pathogens that infect plants. Pathogenic fungi, bacteria, viruses, viroids, and phytoplasmas are notorious and hard to control; preventive measures are important for managing disease as early as possible. Age-old management practices are time-consuming and labour-intensive processes. In the past, nucleic acid-based methods, such as hybridization, amplification, and sequencing, have been used extensively for the preliminary identification of plant pathogens. Recently, PCR-based methods have been widely used for the detection of plant pathogens. However, PCR methods are time-bound and require high-quality DNA extraction because of inhibitors' effects on PCR sensitivity. Several isothermal detection techniques are commonly used for the onsite detection of plant pathogens. Among them, loop-mediated isothermal amplification (LAMP) is a paradigm diagnostic tool for early plant pathogen detection. Hence, in this review, we discuss the rapid, reliable, sensitive method of the LAMP assay and the limit of detection (LOD) in different sectors of plant pathology. We also address the advantages and disadvantages of different LAMP approaches and future prospects. |
| format | Article |
| id | doaj-art-b514f4fb40fa4b058a6ab59c8847565e |
| institution | Kabale University |
| issn | 1212-2580 1805-9341 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj-art-b514f4fb40fa4b058a6ab59c8847565e2025-01-16T13:05:47ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412025-01-0161112010.17221/62/2024-PPSpps-202501-0001Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogensGovindan Muthukumar0Ayyanar Kamalakannan1Irudhayasamy Johnson2Pachamuthu Kamaraj3Iyyamperumal Muthuvel4Shanmugam Varanavasiappan5Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Fruits, Tamil Nadu Agricultural University, Coimbatore, IndiaDepartment of Farm Machinery and Power Engineering, Agricultural Engineering College & Research Institute, Kumulur, Trichy, IndiaDepartment of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, IndiaPhytopathology deals with a branch of biology encompassing pathogens that infect plants. Pathogenic fungi, bacteria, viruses, viroids, and phytoplasmas are notorious and hard to control; preventive measures are important for managing disease as early as possible. Age-old management practices are time-consuming and labour-intensive processes. In the past, nucleic acid-based methods, such as hybridization, amplification, and sequencing, have been used extensively for the preliminary identification of plant pathogens. Recently, PCR-based methods have been widely used for the detection of plant pathogens. However, PCR methods are time-bound and require high-quality DNA extraction because of inhibitors' effects on PCR sensitivity. Several isothermal detection techniques are commonly used for the onsite detection of plant pathogens. Among them, loop-mediated isothermal amplification (LAMP) is a paradigm diagnostic tool for early plant pathogen detection. Hence, in this review, we discuss the rapid, reliable, sensitive method of the LAMP assay and the limit of detection (LOD) in different sectors of plant pathology. We also address the advantages and disadvantages of different LAMP approaches and future prospects.https://pps.agriculturejournals.cz/artkey/pps-202501-0001_loop-mediated-isothermal-amplification-and-its-limit-of-detection-for-diagnostics-of-plant-pathogens.phppcronsite detectionlampsensitivitydiagnosticslod |
| spellingShingle | Govindan Muthukumar Ayyanar Kamalakannan Irudhayasamy Johnson Pachamuthu Kamaraj Iyyamperumal Muthuvel Shanmugam Varanavasiappan Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens Plant Protection Science pcr onsite detection lamp sensitivity diagnostics lod |
| title | Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| title_full | Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| title_fullStr | Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| title_full_unstemmed | Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| title_short | Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| title_sort | loop mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens |
| topic | pcr onsite detection lamp sensitivity diagnostics lod |
| url | https://pps.agriculturejournals.cz/artkey/pps-202501-0001_loop-mediated-isothermal-amplification-and-its-limit-of-detection-for-diagnostics-of-plant-pathogens.php |
| work_keys_str_mv | AT govindanmuthukumar loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens AT ayyanarkamalakannan loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens AT irudhayasamyjohnson loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens AT pachamuthukamaraj loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens AT iyyamperumalmuthuvel loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens AT shanmugamvaranavasiappan loopmediatedisothermalamplificationanditslimitofdetectionfordiagnosticsofplantpathogens |