An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption
Osteoclasts are terminally differentiated multinucleated giant cells that mediate bone resorption and regulate skeletal homeostasis under physiological and pathological states. Excessive osteoclast activity will give rise to enhanced bone resorption, being responsible for a wide range of metabolic s...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2024-11-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5100&type=0 |
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| author | Xiaoyue Sun Zijun Wang Yi Tang Stephen Weiss Lingxin Zhu |
| author_facet | Xiaoyue Sun Zijun Wang Yi Tang Stephen Weiss Lingxin Zhu |
| author_sort | Xiaoyue Sun |
| collection | DOAJ |
| description | Osteoclasts are terminally differentiated multinucleated giant cells that mediate bone resorption and regulate skeletal homeostasis under physiological and pathological states. Excessive osteoclast activity will give rise to enhanced bone resorption, being responsible for a wide range of metabolic skeletal diseases, ranging from osteoporosis and rheumatoid arthritis to tumor-induced osteolysis. Therefore, the construction of in vitro models of osteoclast-mediated bone resorption is helpful to better understand the functional status of osteoclasts under (patho)physiological conditions. Notably, it is essential to provide an in vivo–relevant bone substrate that induces osteoclasts to generate authentic resorption lacunae and excavate bone. Here, we summarize the experimental design of a reproducible and cost-effective method, which is suitable for evaluating the regulatory mechanisms and influence of molecular agonists and antagonists as well as therapeutics on osteoclast-mediated bone-resorbing activity. |
| format | Article |
| id | doaj-art-b50e3ff918d446a3927d1447b2e58492 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-b50e3ff918d446a3927d1447b2e584922024-12-26T05:17:10ZengBio-protocol LLCBio-Protocol2331-83252024-11-01142110.21769/BioProtoc.5100An In Vitro Model of Murine Osteoclast-Mediated Bone ResorptionXiaoyue Sun0Zijun Wang1Yi Tang2Stephen Weiss3Lingxin Zhu4State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaDivision of Genetic Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USALife Sciences Institute, University of Michigan, Ann Arbor, MI, USADivision of Genetic Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USALife Sciences Institute, University of Michigan, Ann Arbor, MI, USAState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, ChinaOsteoclasts are terminally differentiated multinucleated giant cells that mediate bone resorption and regulate skeletal homeostasis under physiological and pathological states. Excessive osteoclast activity will give rise to enhanced bone resorption, being responsible for a wide range of metabolic skeletal diseases, ranging from osteoporosis and rheumatoid arthritis to tumor-induced osteolysis. Therefore, the construction of in vitro models of osteoclast-mediated bone resorption is helpful to better understand the functional status of osteoclasts under (patho)physiological conditions. Notably, it is essential to provide an in vivo–relevant bone substrate that induces osteoclasts to generate authentic resorption lacunae and excavate bone. Here, we summarize the experimental design of a reproducible and cost-effective method, which is suitable for evaluating the regulatory mechanisms and influence of molecular agonists and antagonists as well as therapeutics on osteoclast-mediated bone-resorbing activity.https://bio-protocol.org/en/bpdetail?id=5100&type=0 |
| spellingShingle | Xiaoyue Sun Zijun Wang Yi Tang Stephen Weiss Lingxin Zhu An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption Bio-Protocol |
| title | An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption |
| title_full | An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption |
| title_fullStr | An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption |
| title_full_unstemmed | An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption |
| title_short | An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption |
| title_sort | in vitro model of murine osteoclast mediated bone resorption |
| url | https://bio-protocol.org/en/bpdetail?id=5100&type=0 |
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