Drosophila miR-263b-5p controls wing developmental growth by targeting Akt
Tissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in Drosophila wing development remains unclear. In this...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2024.2444366 |
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| _version_ | 1846110271803752448 |
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| author | Chae Jeong Kim Daegyu Jang Do-Hwan Lim |
| author_facet | Chae Jeong Kim Daegyu Jang Do-Hwan Lim |
| author_sort | Chae Jeong Kim |
| collection | DOAJ |
| description | Tissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in Drosophila wing development remains unclear. In this study, we found that the wing size of adult flies was negatively affected by miR-263b expression. The miR-263b-mediated alteration in wing size was linked to a reduction in wing cell number. Additionally, miR-263b overexpression in Drosophila S2 cells decreased cell proliferation and increased cell death. Consequently, we identified Akt as a direct target of miR-263b-5p and found that miR-263b-mediated wing growth regulation was due to changes in Akt expression. Co-expression of Akt in miR-263b-overexpressing wings rescued the miR-263b overexpression-mediated reduction in wing growth. These results enhance our understanding of the crucial role of miRNAs in growth regulation during Drosophila wing development. |
| format | Article |
| id | doaj-art-b4f03753565643d4a52c8fce0bcf070b |
| institution | Kabale University |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-b4f03753565643d4a52c8fce0bcf070b2024-12-24T11:00:42ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852025-12-01291354510.1080/19768354.2024.2444366Drosophila miR-263b-5p controls wing developmental growth by targeting AktChae Jeong Kim0Daegyu Jang1Do-Hwan Lim2School of Systems Biomedical Science, Soongsil University, Seoul, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul, Republic of KoreaSchool of Systems Biomedical Science, Soongsil University, Seoul, Republic of KoreaTissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in Drosophila wing development remains unclear. In this study, we found that the wing size of adult flies was negatively affected by miR-263b expression. The miR-263b-mediated alteration in wing size was linked to a reduction in wing cell number. Additionally, miR-263b overexpression in Drosophila S2 cells decreased cell proliferation and increased cell death. Consequently, we identified Akt as a direct target of miR-263b-5p and found that miR-263b-mediated wing growth regulation was due to changes in Akt expression. Co-expression of Akt in miR-263b-overexpressing wings rescued the miR-263b overexpression-mediated reduction in wing growth. These results enhance our understanding of the crucial role of miRNAs in growth regulation during Drosophila wing development.https://www.tandfonline.com/doi/10.1080/19768354.2024.2444366DrosophilamiR-263bAktwinggrowth |
| spellingShingle | Chae Jeong Kim Daegyu Jang Do-Hwan Lim Drosophila miR-263b-5p controls wing developmental growth by targeting Akt Animal Cells and Systems Drosophila miR-263b Akt wing growth |
| title | Drosophila miR-263b-5p controls wing developmental growth by targeting Akt |
| title_full | Drosophila miR-263b-5p controls wing developmental growth by targeting Akt |
| title_fullStr | Drosophila miR-263b-5p controls wing developmental growth by targeting Akt |
| title_full_unstemmed | Drosophila miR-263b-5p controls wing developmental growth by targeting Akt |
| title_short | Drosophila miR-263b-5p controls wing developmental growth by targeting Akt |
| title_sort | drosophila mir 263b 5p controls wing developmental growth by targeting akt |
| topic | Drosophila miR-263b Akt wing growth |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2024.2444366 |
| work_keys_str_mv | AT chaejeongkim drosophilamir263b5pcontrolswingdevelopmentalgrowthbytargetingakt AT daegyujang drosophilamir263b5pcontrolswingdevelopmentalgrowthbytargetingakt AT dohwanlim drosophilamir263b5pcontrolswingdevelopmentalgrowthbytargetingakt |