POLARIZATION REPRODUCTION OF STRUCTURAL ANISOTROPY MAPS OF BRAIN PREPARATIONS FOR DIFFERENTIAL DIAGNOSTICS OF HEMORRHAGES OF TRAUMATIC AND NON-TRAUMATIC GENESIS
In recent years, modern non-destructive and non-invasive methods of optical diagnostics of biological tissue and fluid preparations have become widely and effectively used in various branches of medicine. Among the wide range of optical methods, one of the most effective has been distinguished - la...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bukovynian State Medical University
2025-04-01
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| Series: | Неонатологія, хірургія та перинатальна медицина |
| Subjects: | |
| Online Access: | https://neonatology.bsmu.edu.ua/article/view/326102 |
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| Summary: | In recent years, modern non-destructive and non-invasive methods of optical diagnostics of biological tissue and fluid preparations have become widely and effectively used in various branches of medicine. Among the wide range of optical methods, one of the most effective has been distinguished - laser polarimetric diagnostics of the polycrystalline structure of biological layers.
The purpose and tasks of the research. To experimentally test and determine a set of markers for differential diagnosis of the cause of death due to the formation of hemorrhages of traumatic and non-traumatic genesis in the substance of the human brain by using the Muller matrix mapping method with algorithmic reproduction of linear birefringence of native histological brain sections.
Research materials and methods. As a research material, native histological sections of human brain taken from the parietal region of head from the deceased, whose cause of death was traumatic hemorrhage - group II (total number n=100), ischemic cerebral infarction - group III (n=100), non-traumatic hemorrhage - group IV (n=100), acute coronary insufficiency - group I - control (n=40), were used. The extracted samples of human brain matter were frozen at a temperature of -70°C and histological sections were then made using a freezing microtome. Further, the obtained samples were studied using a Stokes polarimeter using the method of azimuthal-invariant experimental measurement of coordinate distributions of the magnitude of the elements of the Muller matrix of native histological sections of the brain from deceased.
Research results. In the process of increasing degeneration and necrosis, the level of macrostructural linear birefringence decreases, as well as the transformation of the topographic hierarchy of the polycrystalline architectonics of the brain. As a result, the corresponding random values and their fluctuations in the coordinate distributions of linear birefringence of the structural elements of the polycrystalline architectonics of the experimental brain samples decrease. The topographic structure of algorithmically reproduced maps becomes less homogeneous, small-scale and statistical due to degeneration and necrosis of neural networks. Based on this, prognostic scenarios of changes in the set of digital statistical, correlation, fractal and wavelet markers that determine the pathological transformation of linear birefringence of neural networks of native histological brain sections were substantiated.
Conclusions. The diagnostic power of the method of azimuthal-invariant Muller matrix microscopy of native histological sections of the brain of deceased persons due to hemorrhages of traumatic and non-traumatic hegemony has been established at an excellent level (up to 97.5%).
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| ISSN: | 2226-1230 2413-4260 |