Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study
Introduction: Antimicrobial photodynamic therapy (aPDT) utilizes light-sensitive materials to inactivate pathogens. Indocyanine green (ICG) is an FDA-approved photosensitizer known for its effective photo-thermal and photo-chemical properties. Aim: This study evaluates the efficacy of ICG-...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2024-12-01
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| Series: | Folia Medica |
| Online Access: | https://foliamedica.bg/article/135281/download/pdf/ |
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| author | Mihail Z. Tanev Georgi T. Tomov Kostadin G. Georgiev Ekaterina D. Georgieva Kamelia V. Petkova-Parlapanska Galina D. Nikolova Yanka D. Karamalakova |
| author_facet | Mihail Z. Tanev Georgi T. Tomov Kostadin G. Georgiev Ekaterina D. Georgieva Kamelia V. Petkova-Parlapanska Galina D. Nikolova Yanka D. Karamalakova |
| author_sort | Mihail Z. Tanev |
| collection | DOAJ |
| description | Introduction: Antimicrobial photodynamic therapy (aPDT) utilizes light-sensitive materials to inactivate pathogens. Indocyanine green (ICG) is an FDA-approved photosensitizer known for its effective photo-thermal and photo-chemical properties. Aim: This study evaluates the efficacy of ICG-based aPDT in eliminating reactive species compared to methylene blue (MtB) using electron paramagnetic resonance (EPR) spectroscopy. Materials and methods: Solid samples of ICG and MtB were prepared at 0.33% concentrations. Solutions were irradiated with lasers at 810 nm and 630 nm, respectively. EPR spectroscopy measured reactive oxygen species (ROS) and reactive nitrogen species (RNS). Spin-trapping agents assessed alkyl radicals, superoxide, and singlet oxygen. Results: ICG demonstrated higher scavenging activity for ROS/RNS compared to MtB. Under PDT, ICG significantly enhanced the reduction of photooxidative stress markers in vitro. Conclusions: ICG combined with aPDT is more effective than MtB in reducing ROS/RNS, indicating its potential for enhanced antimicrobial applications. |
| format | Article |
| id | doaj-art-b40b653c248b422dbb3e147ea86d4608 |
| institution | Kabale University |
| issn | 1314-2143 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj-art-b40b653c248b422dbb3e147ea86d46082025-01-07T08:30:11ZengPensoft PublishersFolia Medica1314-21432024-12-0166687688310.3897/folmed.66.e135281135281Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro studyMihail Z. Tanev0Georgi T. Tomov1Kostadin G. Georgiev2Ekaterina D. Georgieva3Kamelia V. Petkova-Parlapanska4Galina D. Nikolova5Yanka D. Karamalakova6Medical University of PlovdivNew Bulgarian UniversityMedical University of PlovdivTrakia UniversityTrakia UniversityTrakia UniversityTrakia UniversityIntroduction: Antimicrobial photodynamic therapy (aPDT) utilizes light-sensitive materials to inactivate pathogens. Indocyanine green (ICG) is an FDA-approved photosensitizer known for its effective photo-thermal and photo-chemical properties. Aim: This study evaluates the efficacy of ICG-based aPDT in eliminating reactive species compared to methylene blue (MtB) using electron paramagnetic resonance (EPR) spectroscopy. Materials and methods: Solid samples of ICG and MtB were prepared at 0.33% concentrations. Solutions were irradiated with lasers at 810 nm and 630 nm, respectively. EPR spectroscopy measured reactive oxygen species (ROS) and reactive nitrogen species (RNS). Spin-trapping agents assessed alkyl radicals, superoxide, and singlet oxygen. Results: ICG demonstrated higher scavenging activity for ROS/RNS compared to MtB. Under PDT, ICG significantly enhanced the reduction of photooxidative stress markers in vitro. Conclusions: ICG combined with aPDT is more effective than MtB in reducing ROS/RNS, indicating its potential for enhanced antimicrobial applications.https://foliamedica.bg/article/135281/download/pdf/ |
| spellingShingle | Mihail Z. Tanev Georgi T. Tomov Kostadin G. Georgiev Ekaterina D. Georgieva Kamelia V. Petkova-Parlapanska Galina D. Nikolova Yanka D. Karamalakova Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study Folia Medica |
| title | Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study |
| title_full | Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study |
| title_fullStr | Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study |
| title_full_unstemmed | Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study |
| title_short | Evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination: an in vitro study |
| title_sort | evaluation of indocyanine green antimicrobial photodynamic therapy in radical species elimination an in vitro study |
| url | https://foliamedica.bg/article/135281/download/pdf/ |
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