MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population
Objective: Micro RNA-21(miR-21) earlier identified as high amount in different types of cancer. They are non-coding sequences with limited no of nucleotides and play important role in regulation of genes. Predicting the miR-21 levels could act as biomarkers for Oral Squamous Cell Carcinoma (OSCC). O...
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Elsevier
2024-03-01
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| Series: | Oral Oncology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906023001292 |
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| author | Chevuru Sai Shreya Reddy Ashikha Shirin Usman P.P Dhanraj M. Ganapathy Ameya K.P. Durairaj Sekar |
| author_facet | Chevuru Sai Shreya Reddy Ashikha Shirin Usman P.P Dhanraj M. Ganapathy Ameya K.P. Durairaj Sekar |
| author_sort | Chevuru Sai Shreya Reddy |
| collection | DOAJ |
| description | Objective: Micro RNA-21(miR-21) earlier identified as high amount in different types of cancer. They are non-coding sequences with limited no of nucleotides and play important role in regulation of genes. Predicting the miR-21 levels could act as biomarkers for Oral Squamous Cell Carcinoma (OSCC). OSCC include every cancer found in the oropharynx and oral cavity. The current study retrieved mature sequences of miRNA from database- NCBI and used to find miRNA from OSCC. Its coming among the list of most prevalent malignancy in world, especially in India. This paper primarily focuses on miR-21 expression analysis as a biomarker of oscc in the South Indian population. Materials and methods: miR-21 secondary structure is predicted using Bioinformatics tools from OSCC. Sequence was extracted from NCBI 25 OSCC samples and adjacent normal tissues were collected, and RNA were extracted, quantified, and reverse transcribed after doing histopathological analysis in cancer tissues. Expression of miR-21 done using qRT-PCR from the cDNA obtained. Utilizing statistical software, the relationship between the expression of miR-21 and the clinicopathological characteristics of OSCC was examined. Level of expression of genes in both cancer cells and normal cells are also analysed using graphical method. Result: miR-21 secondary structure is predicted by applying mature sequence of miRNA. The values obtained by statistical analysis showed expression of miR-21 is increased in oscc samples compared to normal cells. Conclusion: Thus miR-21 is a potential biomarker associated with OSCC. Study also elucidated positive correlation between miR-21 in oscc samples. |
| format | Article |
| id | doaj-art-b405df81a6f54d7bab0c10046ef128c5 |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-b405df81a6f54d7bab0c10046ef128c52025-01-09T06:15:06ZengElsevierOral Oncology Reports2772-90602024-03-019100139MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian populationChevuru Sai Shreya Reddy0Ashikha Shirin Usman P.P1Dhanraj M. Ganapathy2Ameya K.P.3Durairaj Sekar4Department of Prosthodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaDepartment of Prosthodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaRNA Biology Lab, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Corresponding author. RNA Biology Lab, Saveetha Dental College & Hospitals, Saveetha Institute of Medical & Technical Sciences (SIMATS), Saveetha University, Chennai, 600077, India.Objective: Micro RNA-21(miR-21) earlier identified as high amount in different types of cancer. They are non-coding sequences with limited no of nucleotides and play important role in regulation of genes. Predicting the miR-21 levels could act as biomarkers for Oral Squamous Cell Carcinoma (OSCC). OSCC include every cancer found in the oropharynx and oral cavity. The current study retrieved mature sequences of miRNA from database- NCBI and used to find miRNA from OSCC. Its coming among the list of most prevalent malignancy in world, especially in India. This paper primarily focuses on miR-21 expression analysis as a biomarker of oscc in the South Indian population. Materials and methods: miR-21 secondary structure is predicted using Bioinformatics tools from OSCC. Sequence was extracted from NCBI 25 OSCC samples and adjacent normal tissues were collected, and RNA were extracted, quantified, and reverse transcribed after doing histopathological analysis in cancer tissues. Expression of miR-21 done using qRT-PCR from the cDNA obtained. Utilizing statistical software, the relationship between the expression of miR-21 and the clinicopathological characteristics of OSCC was examined. Level of expression of genes in both cancer cells and normal cells are also analysed using graphical method. Result: miR-21 secondary structure is predicted by applying mature sequence of miRNA. The values obtained by statistical analysis showed expression of miR-21 is increased in oscc samples compared to normal cells. Conclusion: Thus miR-21 is a potential biomarker associated with OSCC. Study also elucidated positive correlation between miR-21 in oscc samples.http://www.sciencedirect.com/science/article/pii/S2772906023001292Oral cancerOral squamous cell carcinoma (OSCC)miRNAmiR-21BiomarkerPrediction marker |
| spellingShingle | Chevuru Sai Shreya Reddy Ashikha Shirin Usman P.P Dhanraj M. Ganapathy Ameya K.P. Durairaj Sekar MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population Oral Oncology Reports Oral cancer Oral squamous cell carcinoma (OSCC) miRNA miR-21 Biomarker Prediction marker |
| title | MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population |
| title_full | MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population |
| title_fullStr | MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population |
| title_full_unstemmed | MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population |
| title_short | MicroRNA-21 as a biomarker in terminal stage oral squamous cell carcinoma (OSCC) in the South Indian population |
| title_sort | microrna 21 as a biomarker in terminal stage oral squamous cell carcinoma oscc in the south indian population |
| topic | Oral cancer Oral squamous cell carcinoma (OSCC) miRNA miR-21 Biomarker Prediction marker |
| url | http://www.sciencedirect.com/science/article/pii/S2772906023001292 |
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