Modification of <i>Saccharomyces cerevisiae</i> Cells with Metal Hexacyanoferrates for the Construction of a Yeast-Based Fuel Cell
This research presents a simple procedure for chemically modifying yeast (<i>Saccharomyces cerevisiae</i>) cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep vol...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/1/137 |
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| Summary: | This research presents a simple procedure for chemically modifying yeast (<i>Saccharomyces cerevisiae</i>) cells with nickel hexacyanoferrate (NiHCF) and ferric hexacyanoferrate, also known as Prussian blue (PB), to increase the conductivity of the yeast cell wall. Using linear sweep voltammetry, NiHCF-modified yeast and PB-modified yeast (NiHCF/yeast and PB/yeast, respectively) were found to have better cell wall conductivity in [Fe(CN)<sub>6</sub>]<sup>3−</sup> and glucose-containing phosphate-buffered solution than unmodified yeast. Spectrophotometric analysis showed that the modification of yeast cells with NiHCF had a less harmful effect on yeast cell viability than the modification of yeast cells with PB. The use of NiHCF/yeast and PB/yeast cells in the construction of a yeast-based fuel cell allowed the maximum power densities of 62.66 mW/m<sup>2</sup> and 94.09 mW/m<sup>2</sup> to be achieved. These values were much higher than those obtained using unmodified yeast cells (42.25 mW/m<sup>2</sup>). NiHCF/yeast and PB/yeast fuel cells were renewed by replenishing the yeast suspension in the anolyte or the FeCl<sub>3</sub> salt in the catholyte. This allowed 77.4% and 50.1% of the initial maximum power density of the fuel cells to be achieved. |
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| ISSN: | 1420-3049 |