Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese
This study aimed to assess the safety and antimicrobial properties of Enterococcus species isolated from raw goat milk and cheese. The isolates were evaluated for hemolytic activity on blood agar plates and antibiotic susceptibility by determining the minimum inhibitory concentrations (MICs) of five...
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| Format: | Article |
| Language: | English |
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University of Kragujevac, Faculty of Science
2024-01-01
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| Series: | Kragujevac Journal of Science |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402049G.pdf |
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| author | Grujović Mirjana Ž. Žugić-Petrović Tanja Marković Katarina G. |
| author_facet | Grujović Mirjana Ž. Žugić-Petrović Tanja Marković Katarina G. |
| author_sort | Grujović Mirjana Ž. |
| collection | DOAJ |
| description | This study aimed to assess the safety and antimicrobial properties of Enterococcus species isolated from raw goat milk and cheese. The isolates were evaluated for hemolytic activity on blood agar plates and antibiotic susceptibility by determining the minimum inhibitory concentrations (MICs) of five antibiotics: ampicillin, tetracycline, gentamicin, streptomycin, and vancomycin. Furthermore, the antagonistic activity of the Enterococcus strains against indicator bacteria was assessed using the agar-well diffusion method. Hemolytic activity results demonstrated a-hemolysis in all isolates. Among the 71 Enterococcus isolates examined, 32.39% exhibited resistance to at least one antibiotic, with resistance profiles varying between species. Enterococcus faecalis isolates predominantly showed resistance to vancomycin, ampicillin, tetracycline, and gentamicin, while Enterococcus faecium and Enterococcus hirae displayed resistance to vancomycin with different susceptibility patterns to the other antibiotics. The antagonistic assays revealed a broad spectrum of inhibitory effects, with variation in inhibition zone diameters. These findings provide valuable insights into the safety and antimicrobial potential of Enterococcus spp. from raw goat milk and cheese and give an insight into their potential applications in the food industry. |
| format | Article |
| id | doaj-art-b3d05fe6c51841f095cbd49406817500 |
| institution | Kabale University |
| issn | 1450-9636 2466-5509 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Kragujevac, Faculty of Science |
| record_format | Article |
| series | Kragujevac Journal of Science |
| spelling | doaj-art-b3d05fe6c51841f095cbd494068175002025-01-08T16:26:54ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092024-01-01462496210.5937/KgJSci2402049G1450-96362402049GSafety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheeseGrujović Mirjana Ž.0https://orcid.org/0000-0002-6174-6717Žugić-Petrović Tanja1https://orcid.org/0000-0001-8237-1080Marković Katarina G.2https://orcid.org/0000-0003-0105-6447University of Kragujevac, Institute for Information Technologies, Kragujevac, SerbiaBio Food Viking, Sokobanja, SerbiaUniversity of Kragujevac, Institute for Information Technologies, Kragujevac, SerbiaThis study aimed to assess the safety and antimicrobial properties of Enterococcus species isolated from raw goat milk and cheese. The isolates were evaluated for hemolytic activity on blood agar plates and antibiotic susceptibility by determining the minimum inhibitory concentrations (MICs) of five antibiotics: ampicillin, tetracycline, gentamicin, streptomycin, and vancomycin. Furthermore, the antagonistic activity of the Enterococcus strains against indicator bacteria was assessed using the agar-well diffusion method. Hemolytic activity results demonstrated a-hemolysis in all isolates. Among the 71 Enterococcus isolates examined, 32.39% exhibited resistance to at least one antibiotic, with resistance profiles varying between species. Enterococcus faecalis isolates predominantly showed resistance to vancomycin, ampicillin, tetracycline, and gentamicin, while Enterococcus faecium and Enterococcus hirae displayed resistance to vancomycin with different susceptibility patterns to the other antibiotics. The antagonistic assays revealed a broad spectrum of inhibitory effects, with variation in inhibition zone diameters. These findings provide valuable insights into the safety and antimicrobial potential of Enterococcus spp. from raw goat milk and cheese and give an insight into their potential applications in the food industry.https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402049G.pdfenterococciantibiotic sensitivityhemolytic activityantimicrobial activitygoat cheese |
| spellingShingle | Grujović Mirjana Ž. Žugić-Petrović Tanja Marković Katarina G. Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese Kragujevac Journal of Science enterococci antibiotic sensitivity hemolytic activity antimicrobial activity goat cheese |
| title | Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese |
| title_full | Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese |
| title_fullStr | Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese |
| title_full_unstemmed | Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese |
| title_short | Safety assessment and antimicrobial potential of Enterococcus spp. isolated from raw goat milk and cheese |
| title_sort | safety assessment and antimicrobial potential of enterococcus spp isolated from raw goat milk and cheese |
| topic | enterococci antibiotic sensitivity hemolytic activity antimicrobial activity goat cheese |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362402049G.pdf |
| work_keys_str_mv | AT grujovicmirjanaz safetyassessmentandantimicrobialpotentialofenterococcussppisolatedfromrawgoatmilkandcheese AT zugicpetrovictanja safetyassessmentandantimicrobialpotentialofenterococcussppisolatedfromrawgoatmilkandcheese AT markovickatarinag safetyassessmentandantimicrobialpotentialofenterococcussppisolatedfromrawgoatmilkandcheese |