Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol
Introduction Current tuberculosis triage and predictive tools offer poor accuracy and are ineffective for detecting asymptomatic disease in people living with HIV (PLHIV). Host tuberculosis transcriptomic biomarkers hold promise for diagnosing prevalent and predicting progression to incident tubercu...
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| Format: | Article |
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BMJ Publishing Group
2021-08-01
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| Series: | BMJ Open |
| Online Access: | https://bmjopen.bmj.com/content/11/8/e048623.full |
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| author | Mary Shelton Humphrey Mulenga Stanley Kimbung Mbandi Simon C Mendelsohn Mark Hatherill Fatoumatta Darboe Thomas J Scriba |
| author_facet | Mary Shelton Humphrey Mulenga Stanley Kimbung Mbandi Simon C Mendelsohn Mark Hatherill Fatoumatta Darboe Thomas J Scriba |
| author_sort | Mary Shelton |
| collection | DOAJ |
| description | Introduction Current tuberculosis triage and predictive tools offer poor accuracy and are ineffective for detecting asymptomatic disease in people living with HIV (PLHIV). Host tuberculosis transcriptomic biomarkers hold promise for diagnosing prevalent and predicting progression to incident tuberculosis and guiding further investigation, preventive therapy and follow-up. We aim to conduct a systematic review of performance of transcriptomic signatures of tuberculosis in PLHIV.Methods and analysis We will search MEDLINE (PubMed), WOS Core Collection, Biological Abstracts, and SciELO Citation Index (Web of Science), Africa-Wide Information and General Science Abstracts (EBSCOhost), Scopus, and Cochrane Central Register of Controlled Trials databases for articles published in English between 1990 and 2020. Case–control, cross-sectional, cohort and randomised controlled studies evaluating performance of diagnostic and prognostic host-response transcriptomic signatures in PLHIV of all ages and settings will be included. Eligible studies will include PLHIV in signature test or validation cohorts, and use microbiological, clinical, or composite reference standards for pulmonary or extrapulmonary tuberculosis diagnosis. Study quality will be evaluated using the ‘Quality Assessment of Diagnostic Accuracy Studies-2’ tool and cumulative review evidence assessed using the ‘Grading of Recommendations Assessment, Development and Evaluation’ approach. Study selection, quality appraisal and data extraction will be performed independently by two reviewers. Study, cohort and signature characteristics of included studies will be tabulated, and a narrative synthesis of findings presented. Primary outcomes of interest, biomarker sensitivity and specificity with estimate precision, will be summarised in forest plots. Expected heterogeneity in signature characteristics, study settings, and study designs precludes meta-analysis and pooling of results. Review reporting will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses of Diagnostic Test Accuracy Studies guidelines.Ethics and dissemination Formal ethics approval is not required as primary human participant data will not be collected. Results will be disseminated through peer-reviewed publication and conference presentation.PROSPERO registration number CRD42021224155. |
| format | Article |
| id | doaj-art-b3b8f5eb08e1446392132255ba04941b |
| institution | Kabale University |
| issn | 2044-6055 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open |
| spelling | doaj-art-b3b8f5eb08e1446392132255ba04941b2024-12-08T06:35:10ZengBMJ Publishing GroupBMJ Open2044-60552021-08-0111810.1136/bmjopen-2021-048623Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocolMary Shelton0Humphrey Mulenga1Stanley Kimbung Mbandi2Simon C Mendelsohn3Mark Hatherill4Fatoumatta Darboe5Thomas J Scriba63 Health Sciences Library, University of Cape Town, Cape Town, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaSouth African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town Faculty of Health Sciences, Cape Town, Western Cape, South AfricaIntroduction Current tuberculosis triage and predictive tools offer poor accuracy and are ineffective for detecting asymptomatic disease in people living with HIV (PLHIV). Host tuberculosis transcriptomic biomarkers hold promise for diagnosing prevalent and predicting progression to incident tuberculosis and guiding further investigation, preventive therapy and follow-up. We aim to conduct a systematic review of performance of transcriptomic signatures of tuberculosis in PLHIV.Methods and analysis We will search MEDLINE (PubMed), WOS Core Collection, Biological Abstracts, and SciELO Citation Index (Web of Science), Africa-Wide Information and General Science Abstracts (EBSCOhost), Scopus, and Cochrane Central Register of Controlled Trials databases for articles published in English between 1990 and 2020. Case–control, cross-sectional, cohort and randomised controlled studies evaluating performance of diagnostic and prognostic host-response transcriptomic signatures in PLHIV of all ages and settings will be included. Eligible studies will include PLHIV in signature test or validation cohorts, and use microbiological, clinical, or composite reference standards for pulmonary or extrapulmonary tuberculosis diagnosis. Study quality will be evaluated using the ‘Quality Assessment of Diagnostic Accuracy Studies-2’ tool and cumulative review evidence assessed using the ‘Grading of Recommendations Assessment, Development and Evaluation’ approach. Study selection, quality appraisal and data extraction will be performed independently by two reviewers. Study, cohort and signature characteristics of included studies will be tabulated, and a narrative synthesis of findings presented. Primary outcomes of interest, biomarker sensitivity and specificity with estimate precision, will be summarised in forest plots. Expected heterogeneity in signature characteristics, study settings, and study designs precludes meta-analysis and pooling of results. Review reporting will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses of Diagnostic Test Accuracy Studies guidelines.Ethics and dissemination Formal ethics approval is not required as primary human participant data will not be collected. Results will be disseminated through peer-reviewed publication and conference presentation.PROSPERO registration number CRD42021224155.https://bmjopen.bmj.com/content/11/8/e048623.full |
| spellingShingle | Mary Shelton Humphrey Mulenga Stanley Kimbung Mbandi Simon C Mendelsohn Mark Hatherill Fatoumatta Darboe Thomas J Scriba Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol BMJ Open |
| title | Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol |
| title_full | Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol |
| title_fullStr | Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol |
| title_full_unstemmed | Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol |
| title_short | Host blood transcriptomic biomarkers of tuberculosis disease in people living with HIV: a systematic review protocol |
| title_sort | host blood transcriptomic biomarkers of tuberculosis disease in people living with hiv a systematic review protocol |
| url | https://bmjopen.bmj.com/content/11/8/e048623.full |
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