Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier
Nicotinamide mononucleotide (NMN) possesses a variety of physiological functions and has therapeutic effects on cardio-cerebral diseases, senile degenerative diseases, neurodegenerative diseases, delayed aging, etc. However, its ability to cross the blood–brain barrier (BBB) and the mechanism of its...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2076-3417/14/24/11732 |
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| author | Tiantian Wang Qi Wu Lihong Wang Tao Lan Zhenyu Yun Lin Zhao Xi Wu |
| author_facet | Tiantian Wang Qi Wu Lihong Wang Tao Lan Zhenyu Yun Lin Zhao Xi Wu |
| author_sort | Tiantian Wang |
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| description | Nicotinamide mononucleotide (NMN) possesses a variety of physiological functions and has therapeutic effects on cardio-cerebral diseases, senile degenerative diseases, neurodegenerative diseases, delayed aging, etc. However, its ability to cross the blood–brain barrier (BBB) and the mechanism of its transport have not been reported. In this study, we used the immortalized hCMEC/D3 cell line to construct an in vitro monolayer cell BBB model, evaluated its ability to cross the blood–brain barrier, and explored the mechanism by carrying out transport and efflux experiments on NMN. The ability of NMN to cross the BBB was investigated by preparing NMN-loaded liposomes conjugated with ANG peptide and RVG peptide. The results showed that the transmembrane transport ability of NMN was moderate, and the transport mechanism was passive transport relying on the concentration difference. The trans-BBB ability of ANG peptide coupled with NMN could be highly significantly improved. |
| format | Article |
| id | doaj-art-b33c0c7aa8aa49c2ba95b2c1eea9d66a |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-b33c0c7aa8aa49c2ba95b2c1eea9d66a2024-12-27T14:08:11ZengMDPI AGApplied Sciences2076-34172024-12-0114241173210.3390/app142411732Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain BarrierTiantian Wang0Qi Wu1Lihong Wang2Tao Lan3Zhenyu Yun4Lin Zhao5Xi Wu6School of Pharmacy, Jiamusi University, Jiamusi 154007, ChinaChina National Institute of Standardization, Beijing 100191, ChinaSchool of Pharmacy, Jiamusi University, Jiamusi 154007, ChinaChina National Institute of Standardization, Beijing 100191, ChinaChina National Institute of Standardization, Beijing 100191, ChinaChina National Institute of Standardization, Beijing 100191, ChinaChina National Institute of Standardization, Beijing 100191, ChinaNicotinamide mononucleotide (NMN) possesses a variety of physiological functions and has therapeutic effects on cardio-cerebral diseases, senile degenerative diseases, neurodegenerative diseases, delayed aging, etc. However, its ability to cross the blood–brain barrier (BBB) and the mechanism of its transport have not been reported. In this study, we used the immortalized hCMEC/D3 cell line to construct an in vitro monolayer cell BBB model, evaluated its ability to cross the blood–brain barrier, and explored the mechanism by carrying out transport and efflux experiments on NMN. The ability of NMN to cross the BBB was investigated by preparing NMN-loaded liposomes conjugated with ANG peptide and RVG peptide. The results showed that the transmembrane transport ability of NMN was moderate, and the transport mechanism was passive transport relying on the concentration difference. The trans-BBB ability of ANG peptide coupled with NMN could be highly significantly improved.https://www.mdpi.com/2076-3417/14/24/11732in vitro blood–brain barrierNMNliposome<i>P<sub>app</sub></i> valuetransport mechanism |
| spellingShingle | Tiantian Wang Qi Wu Lihong Wang Tao Lan Zhenyu Yun Lin Zhao Xi Wu Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier Applied Sciences in vitro blood–brain barrier NMN liposome <i>P<sub>app</sub></i> value transport mechanism |
| title | Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier |
| title_full | Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier |
| title_fullStr | Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier |
| title_full_unstemmed | Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier |
| title_short | Construction of Nicotinamide Mononucleotide-Loaded Liposomes and Their In Vitro Transport Across the Blood–Brain Barrier |
| title_sort | construction of nicotinamide mononucleotide loaded liposomes and their in vitro transport across the blood brain barrier |
| topic | in vitro blood–brain barrier NMN liposome <i>P<sub>app</sub></i> value transport mechanism |
| url | https://www.mdpi.com/2076-3417/14/24/11732 |
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