Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids
In the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/12/594 |
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| author | Jiyu Han Yejin Lee Jin-Ho Lee Jinho Yoon |
| author_facet | Jiyu Han Yejin Lee Jin-Ho Lee Jinho Yoon |
| author_sort | Jiyu Han |
| collection | DOAJ |
| description | In the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a redox indicator, fluorescent dye, or quencher for biosensing. To avoid this complex chemical modification, in this research, mismatched DNA with an intercalated metal ion complex (MIMIC) is employed as the probe sequence. In addition, the MIMIC is fabricated on a lithography-assisted nanostructure-modified flexible polymer electrode. On this flexible electrode, as a proof-of-concept study, a human papillomavirus (HPV-16 and -18) was detected by the MIMIC with a high accuracy. The developed biosensor exhibits an ultrasensitive ability to detect HPV in viral DNA without target amplification and chemical modifications in a simple preparation manner. Moreover, it retains its nanostructures and high conductivity after bending. In conclusion, the use of the proposed biosensor suggests a novel approach to developing an ultrasensitive and flexible biosensor for the detection of important biomarkers in a simple manner that can be applied in point-of-care testing. |
| format | Article |
| id | doaj-art-b32aa18a5e4640b39042e2e14aa29747 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-b32aa18a5e4640b39042e2e14aa297472024-12-27T14:14:13ZengMDPI AGBiosensors2079-63742024-12-01141259410.3390/bios14120594Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic AcidsJiyu Han0Yejin Lee1Jin-Ho Lee2Jinho Yoon3Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of KoreaDepartment of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of KoreaSchool of Biomedical Convergence Engineering, Pusan National University, Yangsan 50612, Republic of KoreaDepartment of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of KoreaIn the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a redox indicator, fluorescent dye, or quencher for biosensing. To avoid this complex chemical modification, in this research, mismatched DNA with an intercalated metal ion complex (MIMIC) is employed as the probe sequence. In addition, the MIMIC is fabricated on a lithography-assisted nanostructure-modified flexible polymer electrode. On this flexible electrode, as a proof-of-concept study, a human papillomavirus (HPV-16 and -18) was detected by the MIMIC with a high accuracy. The developed biosensor exhibits an ultrasensitive ability to detect HPV in viral DNA without target amplification and chemical modifications in a simple preparation manner. Moreover, it retains its nanostructures and high conductivity after bending. In conclusion, the use of the proposed biosensor suggests a novel approach to developing an ultrasensitive and flexible biosensor for the detection of important biomarkers in a simple manner that can be applied in point-of-care testing.https://www.mdpi.com/2079-6374/14/12/594flexible biosensornanotechnologyviral nucleic acidselectrochemistryhuman papillomavirus |
| spellingShingle | Jiyu Han Yejin Lee Jin-Ho Lee Jinho Yoon Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids Biosensors flexible biosensor nanotechnology viral nucleic acids electrochemistry human papillomavirus |
| title | Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids |
| title_full | Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids |
| title_fullStr | Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids |
| title_full_unstemmed | Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids |
| title_short | Flexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids |
| title_sort | flexible electrochemical biosensor using nanostructure modified polymer electrode for detection of viral nucleic acids |
| topic | flexible biosensor nanotechnology viral nucleic acids electrochemistry human papillomavirus |
| url | https://www.mdpi.com/2079-6374/14/12/594 |
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