Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols
Pseudomonas fluorescens strain D7 (P.f. D7) is a naturally occurring soil bacterium that shows promise as a biological herbicide to inhibit growth of annual grass weeds, including downy brome (Bromus tectorum L.), in crop- and rangelands. Pseudomonas fluorescens strain D7rif (P.f. D7rif) is a rifamp...
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| Format: | Article |
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Wiley
2014-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2014/306348 |
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| author | Tami L. Stubbs Ann C. Kennedy Horace D. Skipper |
| author_facet | Tami L. Stubbs Ann C. Kennedy Horace D. Skipper |
| author_sort | Tami L. Stubbs |
| collection | DOAJ |
| description | Pseudomonas fluorescens strain D7 (P.f. D7) is a naturally occurring soil bacterium that shows promise as a biological herbicide to inhibit growth of annual grass weeds, including downy brome (Bromus tectorum L.), in crop- and rangelands. Pseudomonas fluorescens strain D7rif (P.f. D7rif) is a rifampicin-resistant strain of P.f. D7. One of the greatest obstacles to successful biological weed control is survival of the organism under field conditions. Nine soils in the taxonomic order of Mollisols, collected from downy brome-infested areas of the Western and Central United States, were inoculated with P.f. D7rif and incubated in the laboratory to determine the effects of soil type, soil properties, incubation temperature, and soil water potential on survival of P.f. D7rif over 63 days. Silt loam soils from Lind, Washington, and Moro, Oregon, sustained the highest P.f. D7rif populations, and recovery was the lowest from Pendleton, Oregon soil. Survival and recovery of P.f. D7rif varied with soil type and temperature but not with the two soil water potentials tested. After 63 days, P.f. D7rif was recovered at levels greater than log 5.5 colony forming units (CFU) g−1 soil from five of the nine test soils, a level adequate to suppress downy brome under field or range conditions. |
| format | Article |
| id | doaj-art-b31c0f711bc5478e8a1b0b32aba735bd |
| institution | Kabale University |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Applied and Environmental Soil Science |
| spelling | doaj-art-b31c0f711bc5478e8a1b0b32aba735bd2025-02-03T05:47:09ZengWileyApplied and Environmental Soil Science1687-76671687-76752014-01-01201410.1155/2014/306348306348Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine MollisolsTami L. Stubbs0Ann C. Kennedy1Horace D. Skipper2Crop and Soil Sciences Department, Washington State University, 221 Johnson Hall, Pullman, WA 99164-6420, USAUSDA-ARS, Land Management and Water Conservation Research Unit, 217 Johnson Hall, Pullman, WA 99164-6421, USADepartment of Entomology, Soils, & Plant Sciences, E249 Poole Agricultural Center, Clemson, SC 29634-0315, USAPseudomonas fluorescens strain D7 (P.f. D7) is a naturally occurring soil bacterium that shows promise as a biological herbicide to inhibit growth of annual grass weeds, including downy brome (Bromus tectorum L.), in crop- and rangelands. Pseudomonas fluorescens strain D7rif (P.f. D7rif) is a rifampicin-resistant strain of P.f. D7. One of the greatest obstacles to successful biological weed control is survival of the organism under field conditions. Nine soils in the taxonomic order of Mollisols, collected from downy brome-infested areas of the Western and Central United States, were inoculated with P.f. D7rif and incubated in the laboratory to determine the effects of soil type, soil properties, incubation temperature, and soil water potential on survival of P.f. D7rif over 63 days. Silt loam soils from Lind, Washington, and Moro, Oregon, sustained the highest P.f. D7rif populations, and recovery was the lowest from Pendleton, Oregon soil. Survival and recovery of P.f. D7rif varied with soil type and temperature but not with the two soil water potentials tested. After 63 days, P.f. D7rif was recovered at levels greater than log 5.5 colony forming units (CFU) g−1 soil from five of the nine test soils, a level adequate to suppress downy brome under field or range conditions.http://dx.doi.org/10.1155/2014/306348 |
| spellingShingle | Tami L. Stubbs Ann C. Kennedy Horace D. Skipper Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols Applied and Environmental Soil Science |
| title | Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols |
| title_full | Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols |
| title_fullStr | Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols |
| title_full_unstemmed | Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols |
| title_short | Survival of a Rifampicin-Resistant Pseudomonas fluorescens Strain in Nine Mollisols |
| title_sort | survival of a rifampicin resistant pseudomonas fluorescens strain in nine mollisols |
| url | http://dx.doi.org/10.1155/2014/306348 |
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