Functional Activity of Neutrophils in Burn Sepsis
A complex effect of thermal trauma on the immune system triggers the tendency to develop and generalize infection in patients with severe thermal trauma. The phagocytic system, which is represented, first of all, by neutrophilic granulocytes, is significantly altered. Objective: to determine the dyn...
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2017-06-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1584 |
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| author | I. V. Obraztsov M. A. Godkov V. V. Kulabukhov G. A. Vladimirova D. Yu. Izmailov E. V. Proskurnina |
| author_facet | I. V. Obraztsov M. A. Godkov V. V. Kulabukhov G. A. Vladimirova D. Yu. Izmailov E. V. Proskurnina |
| author_sort | I. V. Obraztsov |
| collection | DOAJ |
| description | A complex effect of thermal trauma on the immune system triggers the tendency to develop and generalize infection in patients with severe thermal trauma. The phagocytic system, which is represented, first of all, by neutrophilic granulocytes, is significantly altered. Objective: to determine the dynamics of changes in the functional activity of neutrophils in patients with thermal trauma, as well as its prognostic significance in the development of purulent-septic complications of a burn disease. Materials and methods. The functional activity of neutrophils was assessed by spontaneous and induced chemiluminescence responses of whole blood sequentially stimulated with phorbol-12-myristate"13-acetate(PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). 34 patients with a burn disease of different severity were dynamically examined during observation. Results. The newly developed technique was applied to study the responses of whole blood of patients with burn disease to stimuli; data on the functional activity of neutrophils depending on various severities of the disease and response dynamics were obtained. Analysis of chemiluminescent curves of the development of respiratory explosion of neutrophils helped to reveal parameters of the immune system state, namely: a one— or two-phase response to a stimulus, a «rapid phase» neutrophil specific activity, and a «slow phase» neutrophil specific activity. Conclusion. The results revealed trends in alterations of functional activity of neutrophils in burn disease. Diagnostic and prognostic indices of the functional activity of neutrophils for assessing the severity of the disease and the prognosis of the septic process were suggested. |
| format | Article |
| id | doaj-art-b28f68bad7a54528983e387df7c37b7f |
| institution | Kabale University |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-b28f68bad7a54528983e387df7c37b7f2025-08-20T03:59:40ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102017-06-01132405110.15360/1813-9779-2017-2-40-511550Functional Activity of Neutrophils in Burn SepsisI. V. Obraztsov0M. A. Godkov1V. V. Kulabukhov2G. A. Vladimirova3D. Yu. Izmailov4E. V. Proskurnina5A. N. Ryzhykh State Scientific Center of Coloproctology, Russian Ministry of HealthN. V. Sklifosovsky Research Institute of First AidA. V. Vishnevsky Institute of Surgery, Russian Ministry of HealthFaculty of Fundamental Medicine,Lomonosov Moscow State UniversityFaculty of Fundamental Medicine,Lomonosov Moscow State UniversityFaculty of Fundamental Medicine,Lomonosov Moscow State UniversityA complex effect of thermal trauma on the immune system triggers the tendency to develop and generalize infection in patients with severe thermal trauma. The phagocytic system, which is represented, first of all, by neutrophilic granulocytes, is significantly altered. Objective: to determine the dynamics of changes in the functional activity of neutrophils in patients with thermal trauma, as well as its prognostic significance in the development of purulent-septic complications of a burn disease. Materials and methods. The functional activity of neutrophils was assessed by spontaneous and induced chemiluminescence responses of whole blood sequentially stimulated with phorbol-12-myristate"13-acetate(PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). 34 patients with a burn disease of different severity were dynamically examined during observation. Results. The newly developed technique was applied to study the responses of whole blood of patients with burn disease to stimuli; data on the functional activity of neutrophils depending on various severities of the disease and response dynamics were obtained. Analysis of chemiluminescent curves of the development of respiratory explosion of neutrophils helped to reveal parameters of the immune system state, namely: a one— or two-phase response to a stimulus, a «rapid phase» neutrophil specific activity, and a «slow phase» neutrophil specific activity. Conclusion. The results revealed trends in alterations of functional activity of neutrophils in burn disease. Diagnostic and prognostic indices of the functional activity of neutrophils for assessing the severity of the disease and the prognosis of the septic process were suggested.https://www.reanimatology.com/rmt/article/view/1584chemiluminescence, neutrophil functional activityburn injurysepsis |
| spellingShingle | I. V. Obraztsov M. A. Godkov V. V. Kulabukhov G. A. Vladimirova D. Yu. Izmailov E. V. Proskurnina Functional Activity of Neutrophils in Burn Sepsis Общая реаниматология chemiluminescence, neutrophil functional activity burn injury sepsis |
| title | Functional Activity of Neutrophils in Burn Sepsis |
| title_full | Functional Activity of Neutrophils in Burn Sepsis |
| title_fullStr | Functional Activity of Neutrophils in Burn Sepsis |
| title_full_unstemmed | Functional Activity of Neutrophils in Burn Sepsis |
| title_short | Functional Activity of Neutrophils in Burn Sepsis |
| title_sort | functional activity of neutrophils in burn sepsis |
| topic | chemiluminescence, neutrophil functional activity burn injury sepsis |
| url | https://www.reanimatology.com/rmt/article/view/1584 |
| work_keys_str_mv | AT ivobraztsov functionalactivityofneutrophilsinburnsepsis AT magodkov functionalactivityofneutrophilsinburnsepsis AT vvkulabukhov functionalactivityofneutrophilsinburnsepsis AT gavladimirova functionalactivityofneutrophilsinburnsepsis AT dyuizmailov functionalactivityofneutrophilsinburnsepsis AT evproskurnina functionalactivityofneutrophilsinburnsepsis |