Optimization and Validation of a QuEChERS-Based Method Combined with Gas Chromatography–Tandem Mass Spectrometry for Analyzing Pesticide Residues in Edible Insect Samples
The increasing popularity of edible insects as a sustainable food source necessitates stringent safety measures to monitor pesticide contamination. This study aimed to assess and enhance a QuEChERS-based extraction method coupled with gas chromatography–tandem mass spectrometry (GC-MS/MS) for the qu...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/11/2293 |
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| Summary: | The increasing popularity of edible insects as a sustainable food source necessitates stringent safety measures to monitor pesticide contamination. This study aimed to assess and enhance a QuEChERS-based extraction method coupled with gas chromatography–tandem mass spectrometry (GC-MS/MS) for the quantification of pesticide residues in edible insects (bamboo caterpillars, house crickets, silkworm pupae, giant water bugs, and grasshoppers) by combining multiple individual insect specimens into a single, homogenized sample—five replicates were tested. The method was optimized by evaluating various extraction parameters and showed strong linearity for all 47 target pesticides, with correlation coefficients (R<sup>2</sup>) ranging from 0.9940 to 0.9999. The limits of detection (LODs) varied between 1 and 10 µg/kg, while the limits of quantification (LOQs) ranged from 10 to 15 µg/kg. Recovery studies conducted at three fortification levels (10, 100, and 500 µg/kg) revealed recoveries ranging from 64.54% to 122.12%, that over 97.87% of the pesticides exhibited satisfactory recoveries within the range of 70–120%, and relative standard deviations (RSDs) below 20%, between 1.86% and 6.02%. Matrix effects (%MEs) range from −33.01% to 24.04%, and to those that experienced no effect. More than 94% of the analytes showed minimal ion suppression or enhancement. These results conform to the SANTE guidelines for monitoring pesticide residues in edible insects, enhancing food safety standards and safeguarding consumer protection. |
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| ISSN: | 1420-3049 |