Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia
Introduction: It is unclear how repeated episodes of HG cause brain injury, but oxidative stress has been suggested to play a role. Non-protein thiols (glutathione, GSH, and cysteine, CYSH) form an important antioxidant defence, and their redox state is dependent on intracellular energy supplies and...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2011-03-01
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| Series: | Pediatric Endocrinology, Diabetes and Metabolism |
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| Online Access: | http://cornetis.pl/pliki/ED/2011/1/ED_2011_1_14.pdf |
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| author | Khalid Hussain Heli Salmi Risto Lapatto |
| author_facet | Khalid Hussain Heli Salmi Risto Lapatto |
| author_sort | Khalid Hussain |
| collection | DOAJ |
| description | Introduction: It is unclear how repeated episodes of HG cause brain injury, but oxidative stress has been suggested to play a role. Non-protein thiols (glutathione, GSH, and cysteine, CYSH) form an important antioxidant defence, and their redox state is dependent on intracellular energy supplies and reducing power which are possibly compromised during low blood glucose concentrations. Aim of study: To study thiol status in children undergoing investigations for hypoglycaemia . We hypothesised that thiol metabolism, dependent on intracellular energy supplies and reducing power, might deteriorate during hypoglycaemic episodes. Material and methods: Seventeen children with suspected hypoglycaemic episodes underwent a diagnostic fast. We measured plasma and erythrocyte GSH and CYSH as well as activities of enzymes related to glutathione metabolism during and after the fast. Results: A positive correlation between plasma glucose and reduced (free) cysteine was observed, but plasma GSH levels did not change significantly. Hypoglycaemia was associated with a rise in erythrocyte total cysteine without changes in erythrocyte GSH. Conclusion: Low blood glucose concentration is associated with changes in thiol status, which compromise the protection against oxidative stress. This further supports the hypothesis of oxidative stress being associated with hypoglycaemia. |
| format | Article |
| id | doaj-art-b2344cad87834ad5bbac611b2f6a5cf9 |
| institution | Kabale University |
| issn | 2081-237X |
| language | English |
| publishDate | 2011-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Pediatric Endocrinology, Diabetes and Metabolism |
| spelling | doaj-art-b2344cad87834ad5bbac611b2f6a5cf92024-12-02T00:47:18ZengTermedia Publishing HousePediatric Endocrinology, Diabetes and Metabolism2081-237X2011-03-011711419Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemiaKhalid HussainHeli SalmiRisto LapattoIntroduction: It is unclear how repeated episodes of HG cause brain injury, but oxidative stress has been suggested to play a role. Non-protein thiols (glutathione, GSH, and cysteine, CYSH) form an important antioxidant defence, and their redox state is dependent on intracellular energy supplies and reducing power which are possibly compromised during low blood glucose concentrations. Aim of study: To study thiol status in children undergoing investigations for hypoglycaemia . We hypothesised that thiol metabolism, dependent on intracellular energy supplies and reducing power, might deteriorate during hypoglycaemic episodes. Material and methods: Seventeen children with suspected hypoglycaemic episodes underwent a diagnostic fast. We measured plasma and erythrocyte GSH and CYSH as well as activities of enzymes related to glutathione metabolism during and after the fast. Results: A positive correlation between plasma glucose and reduced (free) cysteine was observed, but plasma GSH levels did not change significantly. Hypoglycaemia was associated with a rise in erythrocyte total cysteine without changes in erythrocyte GSH. Conclusion: Low blood glucose concentration is associated with changes in thiol status, which compromise the protection against oxidative stress. This further supports the hypothesis of oxidative stress being associated with hypoglycaemia.http://cornetis.pl/pliki/ED/2011/1/ED_2011_1_14.pdf glutathionehypoglycaemiaoxidative stressredox state |
| spellingShingle | Khalid Hussain Heli Salmi Risto Lapatto Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia Pediatric Endocrinology, Diabetes and Metabolism glutathione hypoglycaemia oxidative stress redox state |
| title | Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| title_full | Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| title_fullStr | Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| title_full_unstemmed | Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| title_short | Change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| title_sort | change in plasma and erythrocyte thiol levels in children undergoing fasting studies for investigation of hypoglycaemia |
| topic | glutathione hypoglycaemia oxidative stress redox state |
| url | http://cornetis.pl/pliki/ED/2011/1/ED_2011_1_14.pdf |
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