Control of virulence by small RNAs in Streptococcus pneumoniae.
Small noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesi...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002788&type=printable |
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| _version_ | 1841527122661736448 |
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| author | Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch |
| author_facet | Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch |
| author_sort | Beth Mann |
| collection | DOAJ |
| description | Small noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesized that the pneumococcus produces many sRNAs and that they would play an important role in pathogenesis. We describe the application of whole-genome transcriptional sequencing to systematically identify the sRNAs of Streptococcus pneumoniae. Using this approach, we have identified 89 putative sRNAs, 56 of which are newly identified. Furthermore, using targeted genetic approaches and Tn-seq transposon screening, we demonstrate that many of the identified sRNAs have important global and niche-specific roles in virulence. These data constitute the most comprehensive analysis of pneumococcal sRNAs and provide the first evidence of the extensive roles of sRNAs in pneumococcal pathogenesis. |
| format | Article |
| id | doaj-art-b1f6e1760cbb44c5a08d464b17d027d8 |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-b1f6e1760cbb44c5a08d464b17d027d82025-01-16T05:31:01ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-0187e100278810.1371/journal.ppat.1002788Control of virulence by small RNAs in Streptococcus pneumoniae.Beth MannTim van OpijnenJianmin WangCaroline ObertYong-Dong WangRobert CarterDaniel J McGoldrickGranger RidoutAndrew CamilliElaine I TuomanenJason W RoschSmall noncoding RNAs (sRNAs) play important roles in gene regulation in both prokaryotes and eukaryotes. Thus far, no sRNA has been assigned a definitive role in virulence in the major human pathogen Streptococcus pneumoniae. Based on the potential coding capacity of intergenic regions, we hypothesized that the pneumococcus produces many sRNAs and that they would play an important role in pathogenesis. We describe the application of whole-genome transcriptional sequencing to systematically identify the sRNAs of Streptococcus pneumoniae. Using this approach, we have identified 89 putative sRNAs, 56 of which are newly identified. Furthermore, using targeted genetic approaches and Tn-seq transposon screening, we demonstrate that many of the identified sRNAs have important global and niche-specific roles in virulence. These data constitute the most comprehensive analysis of pneumococcal sRNAs and provide the first evidence of the extensive roles of sRNAs in pneumococcal pathogenesis.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002788&type=printable |
| spellingShingle | Beth Mann Tim van Opijnen Jianmin Wang Caroline Obert Yong-Dong Wang Robert Carter Daniel J McGoldrick Granger Ridout Andrew Camilli Elaine I Tuomanen Jason W Rosch Control of virulence by small RNAs in Streptococcus pneumoniae. PLoS Pathogens |
| title | Control of virulence by small RNAs in Streptococcus pneumoniae. |
| title_full | Control of virulence by small RNAs in Streptococcus pneumoniae. |
| title_fullStr | Control of virulence by small RNAs in Streptococcus pneumoniae. |
| title_full_unstemmed | Control of virulence by small RNAs in Streptococcus pneumoniae. |
| title_short | Control of virulence by small RNAs in Streptococcus pneumoniae. |
| title_sort | control of virulence by small rnas in streptococcus pneumoniae |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002788&type=printable |
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