The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats
Abstract Background Recently, silver nanoparticles (Ag-NPs) were shown to provoke oxidative stress through the release of reactive oxygen species and consequently induce cell damage. Selenium-loaded chitosan nanoparticles (CS-SeNPs) have anti-inflammatory and antioxidant effects, indicating that the...
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2025-01-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-024-01577-z |
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| author | Omnia E. Shalaby Yasmine H. Ahmed Aya M. Mekkawy Mohamed Y. Mahmoud G. A. Elbargeesy |
| author_facet | Omnia E. Shalaby Yasmine H. Ahmed Aya M. Mekkawy Mohamed Y. Mahmoud G. A. Elbargeesy |
| author_sort | Omnia E. Shalaby |
| collection | DOAJ |
| description | Abstract Background Recently, silver nanoparticles (Ag-NPs) were shown to provoke oxidative stress through the release of reactive oxygen species and consequently induce cell damage. Selenium-loaded chitosan nanoparticles (CS-SeNPs) have anti-inflammatory and antioxidant effects, indicating that they ameliorate Ag-NPs-induced ovarian toxicity. Objective This study aimed to assess how well CS-SeNPs counteract the damaging effects of Ag-NPs on the ovarian tissue of adult female albino rats. Methods Forty mature female albino rats were divided into four equal groups: for 60 days, Group I (control) was given 0.5 ml/kg of distilled water; Group II was given Ag-NPs orally (100 mg/kg); Group III was given Ag-NPs orally (100 mg/kg/d) plus CS-SeNPs (0.5 mg/kg/d); and Group IV was given only CS-SeNPs orally (0.5 mg/kg/d). All the ovarian tissues were removed and underwent immunohistochemical, histological, and biochemical analyses. Results Ag-NPs-exposed rats revealed a marked reduction in reduced glutathione (GSH) and superoxide dismutase (SOD). Numerous histopathological alterations were found along with a significant increase in PCNA- and Caspase-3-immunoreactive cells. Most of these alterations were successfully ameliorated by CS-SeNPs, as indicated by marked increases in GSH and SOD. Conclusion CS-SeNPs ameliorate the toxic effects of Ag-NPs on the ovarian tissue of adult female albino rats. |
| format | Article |
| id | doaj-art-b1f6b4efe617497b95ce4afcd4b9483f |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-b1f6b4efe617497b95ce4afcd4b9483f2025-01-12T12:35:24ZengBMCJournal of Ovarian Research1757-22152025-01-0118111310.1186/s13048-024-01577-zThe ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino ratsOmnia E. Shalaby0Yasmine H. Ahmed1Aya M. Mekkawy2Mohamed Y. Mahmoud3G. A. Elbargeesy4Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Cairo UniversityAbstract Background Recently, silver nanoparticles (Ag-NPs) were shown to provoke oxidative stress through the release of reactive oxygen species and consequently induce cell damage. Selenium-loaded chitosan nanoparticles (CS-SeNPs) have anti-inflammatory and antioxidant effects, indicating that they ameliorate Ag-NPs-induced ovarian toxicity. Objective This study aimed to assess how well CS-SeNPs counteract the damaging effects of Ag-NPs on the ovarian tissue of adult female albino rats. Methods Forty mature female albino rats were divided into four equal groups: for 60 days, Group I (control) was given 0.5 ml/kg of distilled water; Group II was given Ag-NPs orally (100 mg/kg); Group III was given Ag-NPs orally (100 mg/kg/d) plus CS-SeNPs (0.5 mg/kg/d); and Group IV was given only CS-SeNPs orally (0.5 mg/kg/d). All the ovarian tissues were removed and underwent immunohistochemical, histological, and biochemical analyses. Results Ag-NPs-exposed rats revealed a marked reduction in reduced glutathione (GSH) and superoxide dismutase (SOD). Numerous histopathological alterations were found along with a significant increase in PCNA- and Caspase-3-immunoreactive cells. Most of these alterations were successfully ameliorated by CS-SeNPs, as indicated by marked increases in GSH and SOD. Conclusion CS-SeNPs ameliorate the toxic effects of Ag-NPs on the ovarian tissue of adult female albino rats.https://doi.org/10.1186/s13048-024-01577-zAg-NPsCS-SeNPsOvarian toxicit yHistopathologyImmunohistochemistry |
| spellingShingle | Omnia E. Shalaby Yasmine H. Ahmed Aya M. Mekkawy Mohamed Y. Mahmoud G. A. Elbargeesy The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats Journal of Ovarian Research Ag-NPs CS-SeNPs Ovarian toxicit y Histopathology Immunohistochemistry |
| title | The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats |
| title_full | The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats |
| title_fullStr | The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats |
| title_full_unstemmed | The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats |
| title_short | The ameliorative effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced ovarian toxicity in female albino rats |
| title_sort | ameliorative effect of selenium loaded chitosan nanoparticles against silver nanoparticles induced ovarian toxicity in female albino rats |
| topic | Ag-NPs CS-SeNPs Ovarian toxicit y Histopathology Immunohistochemistry |
| url | https://doi.org/10.1186/s13048-024-01577-z |
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