Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL
BACKGROUND: Chronic lymphoproliferative disorders (LPDs), such as chronic lymphocytic leukemia (CLL) and non-Hodgkin lymphoma (NHL), are comprised heterogeneous malignant hematopoietic clinicopathologic syndromes with broad clinical features. Molecular diagnostic procedures such as immunophenotyping...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Applied Hematology |
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| Online Access: | https://journals.lww.com/10.4103/joah.joah_103_24 |
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| author | Teeb M. Jaafar Al-Khafaji Ahmed Ibraheem Rashid |
| author_facet | Teeb M. Jaafar Al-Khafaji Ahmed Ibraheem Rashid |
| author_sort | Teeb M. Jaafar Al-Khafaji |
| collection | DOAJ |
| description | BACKGROUND:
Chronic lymphoproliferative disorders (LPDs), such as chronic lymphocytic leukemia (CLL) and non-Hodgkin lymphoma (NHL), are comprised heterogeneous malignant hematopoietic clinicopathologic syndromes with broad clinical features. Molecular diagnostic procedures such as immunophenotyping and cytogenetics have improved the accuracy of diagnosis in recent years. While CLL and NHL were previously indistinguishable based on clinical or immunophenotypic parameters, new biomarkers such as CD200 and CD43 have facilitated separation from one another allowing for prognostication in these hematological disorders.
AIM OF STUDY:
To evaluate the diagnostic and prognostic significance of CD200 and CD43 expression in CLL and the leukemic phase of NHL.
SUBJECTS AND METHODS:
The patients studied were 145 who have chronic lymphoproliferative disorders that attended the Baghdad Teaching Hospital at Medical City from (January 1, 2020, to December 31, 2020; they were divided into CLL and NHL groups observed in this cross-sectional study. CD200 and CD43 were assessed as baseline. Clinical and laboratory results were collected, combining CLL staging with the Ann Arbor method and NHL staging with the modified Rai system, respectively.
RESULTS:
CD200 and CD43 were found to have a significant correlation in distinguishing between CLL and NHL. Most CD200-positive patients have moderate to extreme levels of CLL expression, whereas NHL patients generally have dim- or moderate-positive expression patterns. CD43 expression is also dim to moderate in most cases in CLL and few NHL cases.
CONCLUSIONS:
This study shows CD200 and CD43 useful in distinguishing CLL from NHL in leukemic phase. These two markers have the highest co-expression in CLL relative to NHL, which suggests their combination might significantly improve diagnostic power. |
| format | Article |
| id | doaj-art-b1a3e4c1e44c4434906c814f478e9bc5 |
| institution | Kabale University |
| issn | 1658-5127 2454-6976 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Applied Hematology |
| spelling | doaj-art-b1a3e4c1e44c4434906c814f478e9bc52025-01-10T11:14:26ZengWolters Kluwer Medknow PublicationsJournal of Applied Hematology1658-51272454-69762024-12-0115430130710.4103/joah.joah_103_24Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHLTeeb M. Jaafar Al-KhafajiAhmed Ibraheem RashidBACKGROUND: Chronic lymphoproliferative disorders (LPDs), such as chronic lymphocytic leukemia (CLL) and non-Hodgkin lymphoma (NHL), are comprised heterogeneous malignant hematopoietic clinicopathologic syndromes with broad clinical features. Molecular diagnostic procedures such as immunophenotyping and cytogenetics have improved the accuracy of diagnosis in recent years. While CLL and NHL were previously indistinguishable based on clinical or immunophenotypic parameters, new biomarkers such as CD200 and CD43 have facilitated separation from one another allowing for prognostication in these hematological disorders. AIM OF STUDY: To evaluate the diagnostic and prognostic significance of CD200 and CD43 expression in CLL and the leukemic phase of NHL. SUBJECTS AND METHODS: The patients studied were 145 who have chronic lymphoproliferative disorders that attended the Baghdad Teaching Hospital at Medical City from (January 1, 2020, to December 31, 2020; they were divided into CLL and NHL groups observed in this cross-sectional study. CD200 and CD43 were assessed as baseline. Clinical and laboratory results were collected, combining CLL staging with the Ann Arbor method and NHL staging with the modified Rai system, respectively. RESULTS: CD200 and CD43 were found to have a significant correlation in distinguishing between CLL and NHL. Most CD200-positive patients have moderate to extreme levels of CLL expression, whereas NHL patients generally have dim- or moderate-positive expression patterns. CD43 expression is also dim to moderate in most cases in CLL and few NHL cases. CONCLUSIONS: This study shows CD200 and CD43 useful in distinguishing CLL from NHL in leukemic phase. These two markers have the highest co-expression in CLL relative to NHL, which suggests their combination might significantly improve diagnostic power.https://journals.lww.com/10.4103/joah.joah_103_24cd200cd43chronic lymphocytic leukemiaflow cytometryimmunophenotypingnon-hodgkin lymphoma |
| spellingShingle | Teeb M. Jaafar Al-Khafaji Ahmed Ibraheem Rashid Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL Journal of Applied Hematology cd200 cd43 chronic lymphocytic leukemia flow cytometry immunophenotyping non-hodgkin lymphoma |
| title | Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL |
| title_full | Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL |
| title_fullStr | Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL |
| title_full_unstemmed | Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL |
| title_short | Assessment of the Diagnostic and Prognostic Values of CD200 and CD43 in CLL and Leukemic Phase of NHL |
| title_sort | assessment of the diagnostic and prognostic values of cd200 and cd43 in cll and leukemic phase of nhl |
| topic | cd200 cd43 chronic lymphocytic leukemia flow cytometry immunophenotyping non-hodgkin lymphoma |
| url | https://journals.lww.com/10.4103/joah.joah_103_24 |
| work_keys_str_mv | AT teebmjaafaralkhafaji assessmentofthediagnosticandprognosticvaluesofcd200andcd43incllandleukemicphaseofnhl AT ahmedibraheemrashid assessmentofthediagnosticandprognosticvaluesofcd200andcd43incllandleukemicphaseofnhl |