Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin

Skin dryness and xerosis are the most common clinical manifestations of different dermatological diseases. At the same time, it was established that the expression of aquaporin 3 (AQP3) is related to the pathogenesis of atopic dermatitis, psoriasis, eczema, and vitiligo. Thus, our study was focused...

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Main Authors: Viktor Filatov, Andrey Varava, Egor Ilin
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Chemistry Proceedings
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Online Access:https://www.mdpi.com/2673-4583/14/1/51
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author Viktor Filatov
Andrey Varava
Egor Ilin
author_facet Viktor Filatov
Andrey Varava
Egor Ilin
author_sort Viktor Filatov
collection DOAJ
description Skin dryness and xerosis are the most common clinical manifestations of different dermatological diseases. At the same time, it was established that the expression of aquaporin 3 (AQP3) is related to the pathogenesis of atopic dermatitis, psoriasis, eczema, and vitiligo. Thus, our study was focused on the search for new molecules and the investigation of their biological activity to accelerate the expression of AQP3 in the skin’s epidermis. Aloin from an <i>Aloe barbadensis</i> leaf extract and trimethylglycine were chosen as new potential candidates using DiffDock computational modelling. These natural molecules demonstrated a good affinity towards the active site of AQP3 with an estimated docking score of −6.2 kcal/mol to −7.7 kcal/mol. Phyto4Health modelling predicted the anti-psoriatic, anti-inflammatory, and immunosuppressant activities that are useful in the treatment of atopic skin diseases. Furthermore, it was shown that the combination of the <i>Aloe barbadensis</i> leaf extract and trimethylglycine in a mass ratio of 1:1 revealed a clear synergetic effect to increase the AQP3 amount up to two times. Thus, the combination of the <i>Aloe barbadensis</i> extract standardized for aloin and trimethylglycine has a promising potential in drug development and the treatment of dryness.
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spelling doaj-art-b19792cae58c4c049bb5397e37295a802024-12-27T14:17:25ZengMDPI AGChemistry Proceedings2673-45832023-11-011415110.3390/ecsoc-27-16088Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in SkinViktor Filatov0Andrey Varava1Egor Ilin2Department of Pharmaceutical Chemistry and Organization of Pharmaceutical Business, Faculty of Basic Medicine, Lomonosov Moscow State University, 119991 Moscow, RussiaScience Center, SkyLab AG, 1066 Lausanne, SwitzerlandDepartment of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaSkin dryness and xerosis are the most common clinical manifestations of different dermatological diseases. At the same time, it was established that the expression of aquaporin 3 (AQP3) is related to the pathogenesis of atopic dermatitis, psoriasis, eczema, and vitiligo. Thus, our study was focused on the search for new molecules and the investigation of their biological activity to accelerate the expression of AQP3 in the skin’s epidermis. Aloin from an <i>Aloe barbadensis</i> leaf extract and trimethylglycine were chosen as new potential candidates using DiffDock computational modelling. These natural molecules demonstrated a good affinity towards the active site of AQP3 with an estimated docking score of −6.2 kcal/mol to −7.7 kcal/mol. Phyto4Health modelling predicted the anti-psoriatic, anti-inflammatory, and immunosuppressant activities that are useful in the treatment of atopic skin diseases. Furthermore, it was shown that the combination of the <i>Aloe barbadensis</i> leaf extract and trimethylglycine in a mass ratio of 1:1 revealed a clear synergetic effect to increase the AQP3 amount up to two times. Thus, the combination of the <i>Aloe barbadensis</i> extract standardized for aloin and trimethylglycine has a promising potential in drug development and the treatment of dryness.https://www.mdpi.com/2673-4583/14/1/51aloe veratrimethylglycineaquaporinsskin hydrationsynergydocking
spellingShingle Viktor Filatov
Andrey Varava
Egor Ilin
Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
Chemistry Proceedings
aloe vera
trimethylglycine
aquaporins
skin hydration
synergy
docking
title Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
title_full Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
title_fullStr Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
title_full_unstemmed Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
title_short Molecular Modelling and In Vitro Research of New Substances for the Targeted Stimulation of AQP3 in Skin
title_sort molecular modelling and in vitro research of new substances for the targeted stimulation of aqp3 in skin
topic aloe vera
trimethylglycine
aquaporins
skin hydration
synergy
docking
url https://www.mdpi.com/2673-4583/14/1/51
work_keys_str_mv AT viktorfilatov molecularmodellingandinvitroresearchofnewsubstancesforthetargetedstimulationofaqp3inskin
AT andreyvarava molecularmodellingandinvitroresearchofnewsubstancesforthetargetedstimulationofaqp3inskin
AT egorilin molecularmodellingandinvitroresearchofnewsubstancesforthetargetedstimulationofaqp3inskin