Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

Abstract Selection of a reliable internal control gene is critical for quantitative real-time PCR, which is a rapid, highly sensitive, and easy method for evaluating gene expression levels in different samples or tissues. Colletotrichum scovillei, which belongs to the C. acutatum species complex, is...

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Bibliographic Details
Main Authors: Guo Geng Jia, Sun Ha Kim, Jiyoung Min, Nelson Villalobos Zamora, Silvia Soto Montero, Dong-Keun Yi, Soo-Yong Kim, Sang-Keun Oh
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Plant Biology
Subjects:
Colletotrichum scovillei
Evaluating gene expression
Growth stages
Stable reference genes
Quantitative real-time PCR
Online Access:https://doi.org/10.1186/s12870-025-06733-1
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