Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro
Jujube (Ziziphus jujuba Mill.) is a highly abundant wild-growing plant in Montenegro. It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively exp...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024173926 |
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| author | Tatjana Perović Biljana Lazović Mirjana Adakalić Ana Džamić Lazar Žarković Uroš Gašić Marina Kostić Jovana Petrović Dejan Stojković Ana Ćirić |
| author_facet | Tatjana Perović Biljana Lazović Mirjana Adakalić Ana Džamić Lazar Žarković Uroš Gašić Marina Kostić Jovana Petrović Dejan Stojković Ana Ćirić |
| author_sort | Tatjana Perović |
| collection | DOAJ |
| description | Jujube (Ziziphus jujuba Mill.) is a highly abundant wild-growing plant in Montenegro. It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively explored. Herein, we used crud methanol extract and three fractions (methylene chloride, n-butanol and aqueous) to asses bioactive features of fruits from this wild growing edible plant, after which we performed the targeted analysis of phenolic compounds of n-butanol fraction by (UHPLC-DAD-MS/MS). Our antioxidant assays showed the highest radical scavenging potential for n-butanol fraction using DPPH and ABTS methods. As for the antimicrobial activity of extract and three fractions, generally aqueous showed the least promising antibacterial and antifungal properties, whereas methylene chloride, methanol and n-butanol fractions showed quite promising antimicrobial potential. E. coli and S. aureus strains were the most susceptible to the compounds present in the methylene chloride and n-butanol fractions with MIC of 0.01–0.025 mg/mL, and MBC 0.025–0.05 mg/mL, along with MRSA strain, which was the most susceptible to the effects of methanol extract with MIC of 0.10 mg/mL and MBC 0.15 mg/mL. The results of antifungal activity showed lower potential to inhibit growth of pathogenic fungi than bacteria, whereas cytotoxicity assay showed extracts have no effects towards HaCaT skin cell line.The n-butanol fraction had the overall most promising activity, and therefore was subjected to more in depth chemical analysis. |
| format | Article |
| id | doaj-art-b17acaf370c24974aa8e1edaca0c51e0 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-b17acaf370c24974aa8e1edaca0c51e02025-01-17T04:51:02ZengElsevierHeliyon2405-84402025-01-01111e41361Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in MontenegroTatjana Perović0Biljana Lazović1Mirjana Adakalić2Ana Džamić3Lazar Žarković4Uroš Gašić5Marina Kostić6Jovana Petrović7Dejan Stojković8Ana Ćirić9Biotechnical Faculty, Centre for Subtropical Cultures, University of Montenegro, Topolica bb, 8500 Bar, MontenegroBiotechnical Faculty, Centre for Subtropical Cultures, University of Montenegro, Topolica bb, 8500 Bar, MontenegroBiotechnical Faculty, Centre for Subtropical Cultures, University of Montenegro, Topolica bb, 8500 Bar, MontenegroUniversity of Belgrade, Faculty of Biology, Studentski trg 16, 11000, Belgrade, SerbiaUniversity of Belgrade, Faculty of Biology, Studentski trg 16, 11000, Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”- National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108, Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”- National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108, Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”- National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108, Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”- National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108, Belgrade, Serbia; Corresponding author.Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”- National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108, Belgrade, Serbia; Corresponding author.Jujube (Ziziphus jujuba Mill.) is a highly abundant wild-growing plant in Montenegro. It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively explored. Herein, we used crud methanol extract and three fractions (methylene chloride, n-butanol and aqueous) to asses bioactive features of fruits from this wild growing edible plant, after which we performed the targeted analysis of phenolic compounds of n-butanol fraction by (UHPLC-DAD-MS/MS). Our antioxidant assays showed the highest radical scavenging potential for n-butanol fraction using DPPH and ABTS methods. As for the antimicrobial activity of extract and three fractions, generally aqueous showed the least promising antibacterial and antifungal properties, whereas methylene chloride, methanol and n-butanol fractions showed quite promising antimicrobial potential. E. coli and S. aureus strains were the most susceptible to the compounds present in the methylene chloride and n-butanol fractions with MIC of 0.01–0.025 mg/mL, and MBC 0.025–0.05 mg/mL, along with MRSA strain, which was the most susceptible to the effects of methanol extract with MIC of 0.10 mg/mL and MBC 0.15 mg/mL. The results of antifungal activity showed lower potential to inhibit growth of pathogenic fungi than bacteria, whereas cytotoxicity assay showed extracts have no effects towards HaCaT skin cell line.The n-butanol fraction had the overall most promising activity, and therefore was subjected to more in depth chemical analysis.http://www.sciencedirect.com/science/article/pii/S2405844024173926Biological activityChemical compositionEdible fruitsZiziphus jujuba |
| spellingShingle | Tatjana Perović Biljana Lazović Mirjana Adakalić Ana Džamić Lazar Žarković Uroš Gašić Marina Kostić Jovana Petrović Dejan Stojković Ana Ćirić Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro Heliyon Biological activity Chemical composition Edible fruits Ziziphus jujuba |
| title | Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro |
| title_full | Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro |
| title_fullStr | Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro |
| title_full_unstemmed | Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro |
| title_short | Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro |
| title_sort | insights into bioactivity guided chemical profiling of ziziphus jujuba mill fruits wild growing in montenegro |
| topic | Biological activity Chemical composition Edible fruits Ziziphus jujuba |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024173926 |
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