A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos

Controlling parasites in zoo animals is a significant challenge, making practical infection prevention methods essential. This study evaluated a novel solution using parasitophagous fungi-treated forage seeds to reduce soil parasite persistence. We conducted two experiments to assess the soil distri...

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Main Authors: José Ángel Hernández, Cándido Viña, João Lozano, Izaro Zubiría, Miguel Baena, Inês Abreu-Ramos, Rami Salmo, Esther Valderrábano, Gustavo Pérez-Anzúrez, Adolfo Paz-Silva, Rodrigo Muíño, Cristiana Cazapal-Monteiro, María Sol Arias, Mercedes Camiña
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Journal of Zoological and Botanical Gardens
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Online Access:https://www.mdpi.com/2673-5636/5/4/54
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author José Ángel Hernández
Cándido Viña
João Lozano
Izaro Zubiría
Miguel Baena
Inês Abreu-Ramos
Rami Salmo
Esther Valderrábano
Gustavo Pérez-Anzúrez
Adolfo Paz-Silva
Rodrigo Muíño
Cristiana Cazapal-Monteiro
María Sol Arias
Mercedes Camiña
author_facet José Ángel Hernández
Cándido Viña
João Lozano
Izaro Zubiría
Miguel Baena
Inês Abreu-Ramos
Rami Salmo
Esther Valderrábano
Gustavo Pérez-Anzúrez
Adolfo Paz-Silva
Rodrigo Muíño
Cristiana Cazapal-Monteiro
María Sol Arias
Mercedes Camiña
author_sort José Ángel Hernández
collection DOAJ
description Controlling parasites in zoo animals is a significant challenge, making practical infection prevention methods essential. This study evaluated a novel solution using parasitophagous fungi-treated forage seeds to reduce soil parasite persistence. We conducted two experiments to assess the soil distribution of the fungi <i>Mucor circinelloides</i> (ovicidal) and <i>Duddingtonia flagrans</i> (larvicidal). Forage seeds were immersed in a submerged culture containing 10<sup>6</sup> spores/mL and subsequently sown in plastic trays (trial 1) and demarcated ground plots (40 × 30 cm) framed with wood (trial 2). Fifteen days later, <i>Ascaris suum</i> and cyathostomin eggs were placed above the germinated vegetation. After a 25–30-day period, the viability of roundworm eggs reduced by 62%, and half of them remained undeveloped; a 64% reduction in the counts of L3 cyathostomins was recorded. In trial 2, viability decreased by 55% in roundworm eggs, with an infectivity rate of 30%, while L3 counts lessened by 57%. It has been concluded that the risk of infection by ascarids and cyathostomins can be reduced by at least half by sowing the soil with forage seeds pre-treated with fungi, providing a practical solution for parasite control in zoos.
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spelling doaj-art-b16a3c31d5904f7fa45c0dfe9ef64de52024-12-27T14:34:40ZengMDPI AGJournal of Zoological and Botanical Gardens2673-56362024-12-015481782710.3390/jzbg5040054A New Soil-Based Solution for Reducing the Persistence of Parasites in ZoosJosé Ángel Hernández0Cándido Viña1João Lozano2Izaro Zubiría3Miguel Baena4Inês Abreu-Ramos5Rami Salmo6Esther Valderrábano7Gustavo Pérez-Anzúrez8Adolfo Paz-Silva9Rodrigo Muíño10Cristiana Cazapal-Monteiro11María Sol Arias12Mercedes Camiña13Control of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainCIISA—Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisbon, PortugalControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock-Ministry of Agriculture and Rural Development, (INIFAP-SADER), Jiutepec 62550, MexicoControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainPropaedeutic Clinic, Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControl of Parasites Group (COPAR, GI-2120), Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainDepartment of Physiology, Faculty of Veterinary, University of Santiago de Compostela, 27002 Lugo, SpainControlling parasites in zoo animals is a significant challenge, making practical infection prevention methods essential. This study evaluated a novel solution using parasitophagous fungi-treated forage seeds to reduce soil parasite persistence. We conducted two experiments to assess the soil distribution of the fungi <i>Mucor circinelloides</i> (ovicidal) and <i>Duddingtonia flagrans</i> (larvicidal). Forage seeds were immersed in a submerged culture containing 10<sup>6</sup> spores/mL and subsequently sown in plastic trays (trial 1) and demarcated ground plots (40 × 30 cm) framed with wood (trial 2). Fifteen days later, <i>Ascaris suum</i> and cyathostomin eggs were placed above the germinated vegetation. After a 25–30-day period, the viability of roundworm eggs reduced by 62%, and half of them remained undeveloped; a 64% reduction in the counts of L3 cyathostomins was recorded. In trial 2, viability decreased by 55% in roundworm eggs, with an infectivity rate of 30%, while L3 counts lessened by 57%. It has been concluded that the risk of infection by ascarids and cyathostomins can be reduced by at least half by sowing the soil with forage seeds pre-treated with fungi, providing a practical solution for parasite control in zoos.https://www.mdpi.com/2673-5636/5/4/54helminthszoological parkfungal-treated seeds<i>Mucor circinelloides</i><i>Duddingtonia flagrans</i>
spellingShingle José Ángel Hernández
Cándido Viña
João Lozano
Izaro Zubiría
Miguel Baena
Inês Abreu-Ramos
Rami Salmo
Esther Valderrábano
Gustavo Pérez-Anzúrez
Adolfo Paz-Silva
Rodrigo Muíño
Cristiana Cazapal-Monteiro
María Sol Arias
Mercedes Camiña
A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
Journal of Zoological and Botanical Gardens
helminths
zoological park
fungal-treated seeds
<i>Mucor circinelloides</i>
<i>Duddingtonia flagrans</i>
title A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
title_full A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
title_fullStr A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
title_full_unstemmed A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
title_short A New Soil-Based Solution for Reducing the Persistence of Parasites in Zoos
title_sort new soil based solution for reducing the persistence of parasites in zoos
topic helminths
zoological park
fungal-treated seeds
<i>Mucor circinelloides</i>
<i>Duddingtonia flagrans</i>
url https://www.mdpi.com/2673-5636/5/4/54
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