Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene
Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125001503 |
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| author | Jie Shi Jiao Yang Huijun Gao Lan Yang Yinpeng Zhang Ansheng Zhao Rui Yang Zhonghong Xiong Jing Yang Bei Gao |
| author_facet | Jie Shi Jiao Yang Huijun Gao Lan Yang Yinpeng Zhang Ansheng Zhao Rui Yang Zhonghong Xiong Jing Yang Bei Gao |
| author_sort | Jie Shi |
| collection | DOAJ |
| description | Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient carrying the DMD gene mutation c.3682delG. The LZUSHi003-A cell line retained this mutation, while expressing pluripotency markers. Additionally, it demonstrated a normal karyotype and could differentiate into three embryonic germ layers. |
| format | Article |
| id | doaj-art-b0ba567e8e3f4baa91c2021c39ed17f5 |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-b0ba567e8e3f4baa91c2021c39ed17f52025-08-20T03:41:43ZengElsevierStem Cell Research1873-50612025-09-018710380010.1016/j.scr.2025.103800Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin geneJie Shi0Jiao Yang1Huijun Gao2Lan Yang3Yinpeng Zhang4Ansheng Zhao5Rui Yang6Zhonghong Xiong7Jing Yang8Bei Gao9Department of Blood Transfusion, The Second Hospital of Lanzhou University, Lanzhou, Gansu, ChinaDepartment of Reproductive Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Blood Transfusion, The Second Hospital of Lanzhou University, Lanzhou, Gansu, ChinaDepartment of Blood Transfusion, The Second Hospital of Lanzhou University, Lanzhou, Gansu, ChinaThe Second Hospital &Clinical Medical School of Lanzhou University, Lanzhou, Gansu, ChinaThe Second Hospital &Clinical Medical School of Lanzhou University, Lanzhou, Gansu, ChinaThe Second Hospital &Clinical Medical School of Lanzhou University, Lanzhou, Gansu, ChinaThe Second Hospital &Clinical Medical School of Lanzhou University, Lanzhou, Gansu, ChinaThe Second Hospital &Clinical Medical School of Lanzhou University, Lanzhou, Gansu, ChinaDepartment of Pharmacy, Gansu Provincial Hospital, Lanzhou, Gansu, China; Corresponding author.Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient carrying the DMD gene mutation c.3682delG. The LZUSHi003-A cell line retained this mutation, while expressing pluripotency markers. Additionally, it demonstrated a normal karyotype and could differentiate into three embryonic germ layers.http://www.sciencedirect.com/science/article/pii/S1873506125001503 |
| spellingShingle | Jie Shi Jiao Yang Huijun Gao Lan Yang Yinpeng Zhang Ansheng Zhao Rui Yang Zhonghong Xiong Jing Yang Bei Gao Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene Stem Cell Research |
| title | Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene |
| title_full | Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene |
| title_fullStr | Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene |
| title_full_unstemmed | Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene |
| title_short | Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene |
| title_sort | generation of a human induced pluripotent stem cell line lzushi003 a from peripheral mononuclear cells of a patient carrying the c 3682delg mutation in the dystrophin gene |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125001503 |
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