Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae
Streptococcus agalactiae is a major causative agent of streptococcosis in Nile tilapia (Oreochromis niloticus) and understanding its etiology is important to ensure the sustainable development of global tilapia farming. Our research group recently observed contrasting disease patterns in animals inf...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1528721/full |
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| author | Renan José Casarotto Appel Renan José Casarotto Appel Karine Nicole Siqueira Ioannis Konstantinidis Maria Isabel Mello Martins Rajesh Joshi Lucienne Garcia Pretto-Giordano Laurival Antônio Vilas-Boas Jorge Manuel de Oliveira Fernandes Jorge Manuel de Oliveira Fernandes |
| author_facet | Renan José Casarotto Appel Renan José Casarotto Appel Karine Nicole Siqueira Ioannis Konstantinidis Maria Isabel Mello Martins Rajesh Joshi Lucienne Garcia Pretto-Giordano Laurival Antônio Vilas-Boas Jorge Manuel de Oliveira Fernandes Jorge Manuel de Oliveira Fernandes |
| author_sort | Renan José Casarotto Appel |
| collection | DOAJ |
| description | Streptococcus agalactiae is a major causative agent of streptococcosis in Nile tilapia (Oreochromis niloticus) and understanding its etiology is important to ensure the sustainable development of global tilapia farming. Our research group recently observed contrasting disease patterns in animals infected with two different S. agalactiae serotypes (Ib and III). To better understand the basis for these divergent responses, we analyzed the brain transcriptome of Nile tilapia following bacterial exposure. Our findings revealed significant variation in the expression of genes involved in immune (e.g., CD209 antigen, granulin, C-X-C motif chemokine 10, prostacyclin synthase, and interleukins) and neuroendocrine (e.g., mmp13a, mmp9, brain aromatase, and pmch) pathways. The serotype Ib strain seems promptly recognized by the host, triggering a potent inflammatory response, whereas the serotype III strain elicited a less immediate response, resulting in more pronounced central nervous system (CNS) symptoms and behavioral effects. To the best of our knowledge, this is the first study to show serotype-specific immune responses to S. agalactiae in Nile tilapia. These findings are important for advancing disease management and control strategies in aquaculture. Identifying different immune reactions triggered by serotypes Ib and III may assist the development of more specific approaches for preventive measures, early detection, and effective treatment against streptococcosis. |
| format | Article |
| id | doaj-art-b0ac9e2a7b4a48d295d80c3ddd7b8948 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-b0ac9e2a7b4a48d295d80c3ddd7b89482025-01-10T10:58:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.15287211528721Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiaeRenan José Casarotto Appel0Renan José Casarotto Appel1Karine Nicole Siqueira2Ioannis Konstantinidis3Maria Isabel Mello Martins4Rajesh Joshi5Lucienne Garcia Pretto-Giordano6Laurival Antônio Vilas-Boas7Jorge Manuel de Oliveira Fernandes8Jorge Manuel de Oliveira Fernandes9Faculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayDepartment of General Biology, State University of Londrina, Londrina, BrazilDepartment of General Biology, State University of Londrina, Londrina, BrazilFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayDepartment of Veterinary Clinics, State University of Londrina, Londrina, BrazilGenoMar Genetics AS, Oslo, NorwayDepartment of Preventive Veterinary Medicine, State University of Londrina, Londrina, BrazilDepartment of General Biology, State University of Londrina, Londrina, BrazilFaculty of Biosciences and Aquaculture, Nord University, Bodø, NorwayDepartment of Renewable Marine Resources, Institute of Marine Sciences (ICM-CSIC), Barcelona, SpainStreptococcus agalactiae is a major causative agent of streptococcosis in Nile tilapia (Oreochromis niloticus) and understanding its etiology is important to ensure the sustainable development of global tilapia farming. Our research group recently observed contrasting disease patterns in animals infected with two different S. agalactiae serotypes (Ib and III). To better understand the basis for these divergent responses, we analyzed the brain transcriptome of Nile tilapia following bacterial exposure. Our findings revealed significant variation in the expression of genes involved in immune (e.g., CD209 antigen, granulin, C-X-C motif chemokine 10, prostacyclin synthase, and interleukins) and neuroendocrine (e.g., mmp13a, mmp9, brain aromatase, and pmch) pathways. The serotype Ib strain seems promptly recognized by the host, triggering a potent inflammatory response, whereas the serotype III strain elicited a less immediate response, resulting in more pronounced central nervous system (CNS) symptoms and behavioral effects. To the best of our knowledge, this is the first study to show serotype-specific immune responses to S. agalactiae in Nile tilapia. These findings are important for advancing disease management and control strategies in aquaculture. Identifying different immune reactions triggered by serotypes Ib and III may assist the development of more specific approaches for preventive measures, early detection, and effective treatment against streptococcosis.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1528721/fullNile tilapiabrainstreptococcosisimmunityaquaculturegene expression |
| spellingShingle | Renan José Casarotto Appel Renan José Casarotto Appel Karine Nicole Siqueira Ioannis Konstantinidis Maria Isabel Mello Martins Rajesh Joshi Lucienne Garcia Pretto-Giordano Laurival Antônio Vilas-Boas Jorge Manuel de Oliveira Fernandes Jorge Manuel de Oliveira Fernandes Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae Frontiers in Immunology Nile tilapia brain streptococcosis immunity aquaculture gene expression |
| title | Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae |
| title_full | Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae |
| title_fullStr | Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae |
| title_full_unstemmed | Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae |
| title_short | Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae |
| title_sort | comparative transcriptome analysis reveals a serotype specific immune response in nile tilapia oreochromis niloticus infected with streptococcus agalactiae |
| topic | Nile tilapia brain streptococcosis immunity aquaculture gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1528721/full |
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