Phytochemical constituents from corn silk and antimicrobial activity of the isolates
Corn silk (Stigma maydis) is one of the traditional medicines for treating many microbial infections. However, there is little literature on the bioactive compounds responsible for these activities. This study was designed to investigate the phytochemical constituents present in corn silk and to scr...
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International Journal of Secondary Metabolite
2024-11-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3165885 |
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| author | James Oppong-kyekyeku Isaac Ayensu Nestor Tordzagla |
| author_facet | James Oppong-kyekyeku Isaac Ayensu Nestor Tordzagla |
| author_sort | James Oppong-kyekyeku |
| collection | DOAJ |
| description | Corn silk (Stigma maydis) is one of the traditional medicines for treating many microbial infections. However, there is little literature on the bioactive compounds responsible for these activities. This study was designed to investigate the phytochemical constituents present in corn silk and to screen the isolated compounds for antimicrobial activity. The pulverized plant sample of 1.14 kg was extracted with 3.6 L of methanol by cold maceration for 3 days. The extract was screened for phytochemicals, followed by isolation of constituent phytochemicals, characterization, and identification of isolated compounds. The isolates were screened for antibacterial and antifungal activities. The phytochemical screening revealed the presence of alkaloids, flavonoids, coumarins, tannins, reducing sugars, saponins, terpenoids, sterols, and cardiac glycosides. Further phytochemical investigation of the chloroformic subfraction of the methanolic extract of the silk led to the isolation of behenic acid and stigmasterol after running column chromatography as well as other chromatographic methods. The identity of the isolated compounds was established based on extensive spectroscopic analyses of their IR, 1D, 2D NMR data and comparing the data to the reported literature. Stigmasterol was active against Staphylococcus aureus and the fungal strain Candida albicans at 25 µg/mL while the mean minimum inhibitory concentration of behenic acid against Staphylococcus aureus was 100 µg/mL, Tinea corporis and Klebsiella pneumoniae were susceptible at 25 µg/mL. The study showed that plant secondary metabolites might be responsible for the reported biological activities of corn silk. It is the first report of behenic acid isolated from corn silk. |
| format | Article |
| id | doaj-art-b08569085b93478d8f14d38d7f738f75 |
| institution | Kabale University |
| issn | 2148-6905 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-b08569085b93478d8f14d38d7f738f752024-12-06T09:58:39ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052024-11-0111460462210.21448/ijsm.1302694618Phytochemical constituents from corn silk and antimicrobial activity of the isolatesJames Oppong-kyekyeku0https://orcid.org/0000-0001-7181-6793Isaac Ayensu1https://orcid.org/0000-0002-0904-7708Nestor Tordzagla2https://orcid.org/0000-0002-6593-5814Kwame Nkrumah University of Science and TechnologyKwame Nkrumah University of Science and TechnologyUniversity for Development StudiesCorn silk (Stigma maydis) is one of the traditional medicines for treating many microbial infections. However, there is little literature on the bioactive compounds responsible for these activities. This study was designed to investigate the phytochemical constituents present in corn silk and to screen the isolated compounds for antimicrobial activity. The pulverized plant sample of 1.14 kg was extracted with 3.6 L of methanol by cold maceration for 3 days. The extract was screened for phytochemicals, followed by isolation of constituent phytochemicals, characterization, and identification of isolated compounds. The isolates were screened for antibacterial and antifungal activities. The phytochemical screening revealed the presence of alkaloids, flavonoids, coumarins, tannins, reducing sugars, saponins, terpenoids, sterols, and cardiac glycosides. Further phytochemical investigation of the chloroformic subfraction of the methanolic extract of the silk led to the isolation of behenic acid and stigmasterol after running column chromatography as well as other chromatographic methods. The identity of the isolated compounds was established based on extensive spectroscopic analyses of their IR, 1D, 2D NMR data and comparing the data to the reported literature. Stigmasterol was active against Staphylococcus aureus and the fungal strain Candida albicans at 25 µg/mL while the mean minimum inhibitory concentration of behenic acid against Staphylococcus aureus was 100 µg/mL, Tinea corporis and Klebsiella pneumoniae were susceptible at 25 µg/mL. The study showed that plant secondary metabolites might be responsible for the reported biological activities of corn silk. It is the first report of behenic acid isolated from corn silk.https://dergipark.org.tr/en/download/article-file/3165885phytochemicalscorn silkbehenic acidstigmasterolantimicrobial activityphytochemicalscorn silkbehenic acidstigmasterolantimicrobial activity |
| spellingShingle | James Oppong-kyekyeku Isaac Ayensu Nestor Tordzagla Phytochemical constituents from corn silk and antimicrobial activity of the isolates International Journal of Secondary Metabolite phytochemicals corn silk behenic acid stigmasterol antimicrobial activity phytochemicals corn silk behenic acid stigmasterol antimicrobial activity |
| title | Phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| title_full | Phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| title_fullStr | Phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| title_full_unstemmed | Phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| title_short | Phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| title_sort | phytochemical constituents from corn silk and antimicrobial activity of the isolates |
| topic | phytochemicals corn silk behenic acid stigmasterol antimicrobial activity phytochemicals corn silk behenic acid stigmasterol antimicrobial activity |
| url | https://dergipark.org.tr/en/download/article-file/3165885 |
| work_keys_str_mv | AT jamesoppongkyekyeku phytochemicalconstituentsfromcornsilkandantimicrobialactivityoftheisolates AT isaacayensu phytochemicalconstituentsfromcornsilkandantimicrobialactivityoftheisolates AT nestortordzagla phytochemicalconstituentsfromcornsilkandantimicrobialactivityoftheisolates |