Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors
<i>Ruta corsica</i> is a rare and endemic plant native to Corsica. Due to its limited distribution and the priority to preserve natural sites, has been insufficiently studied. In vitro cultures provide an opportunity to research <i>R. corsica</i> under controlled conditions....
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2024-11-01
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| author | Agnieszka Szewczyk Monika Trepa Dominika Zych |
| author_facet | Agnieszka Szewczyk Monika Trepa Dominika Zych |
| author_sort | Agnieszka Szewczyk |
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| description | <i>Ruta corsica</i> is a rare and endemic plant native to Corsica. Due to its limited distribution and the priority to preserve natural sites, has been insufficiently studied. In vitro cultures provide an opportunity to research <i>R. corsica</i> under controlled conditions. In the present study, in vitro cultures of <i>R. corsica</i> were conducted in Plantform<sup>TM</sup> bioreactors. The study aimed to assess the effects of growth cycle length (5 and 6 weeks) and different concentrations of plant growth regulators (NAA and BAP) at 0.1/0.1, 0.1/0.5, 0.5/0.5, 0.5/1.0, and 1.0/1.0 mg/L on biomass growth and secondary metabolite accumulation. HPLC analysis identified compounds in the furanocoumarin and furoquinoline alkaloid groups, with furanocoumarins being the primary secondary metabolites (maximum total content: 1571.5 mg/100 g DW). Among them, xanthotoxin, psoralen, and bergapten were dominant, with maximum concentrations of 588.1, 426.6, and 325.2 mg/100 g DW, respectively. The maximum total content of furoquinoline alkaloids was 661 mg/100 g DW, with γ-fagarine as the primary metabolite, reaching 448 mg/100 g DW. The optimal conditions for secondary metabolite accumulation in <i>R. corsica</i> cultures were a 5-week growth cycle and the LS 0.1/0.1 medium variant. |
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| institution | Kabale University |
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| spelling | doaj-art-b07aa28f5e2e44bf987d940b8b7f93a02024-11-26T18:15:20ZengMDPI AGMolecules1420-30492024-11-012922526110.3390/molecules29225261Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion BioreactorsAgnieszka Szewczyk0Monika Trepa1Dominika Zych2Department of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Krakow, PolandDepartment of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Krakow, PolandSSG of Medicinal Plant and Mushroom Biotechnology, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna St., 30-688 Krakow, Poland<i>Ruta corsica</i> is a rare and endemic plant native to Corsica. Due to its limited distribution and the priority to preserve natural sites, has been insufficiently studied. In vitro cultures provide an opportunity to research <i>R. corsica</i> under controlled conditions. In the present study, in vitro cultures of <i>R. corsica</i> were conducted in Plantform<sup>TM</sup> bioreactors. The study aimed to assess the effects of growth cycle length (5 and 6 weeks) and different concentrations of plant growth regulators (NAA and BAP) at 0.1/0.1, 0.1/0.5, 0.5/0.5, 0.5/1.0, and 1.0/1.0 mg/L on biomass growth and secondary metabolite accumulation. HPLC analysis identified compounds in the furanocoumarin and furoquinoline alkaloid groups, with furanocoumarins being the primary secondary metabolites (maximum total content: 1571.5 mg/100 g DW). Among them, xanthotoxin, psoralen, and bergapten were dominant, with maximum concentrations of 588.1, 426.6, and 325.2 mg/100 g DW, respectively. The maximum total content of furoquinoline alkaloids was 661 mg/100 g DW, with γ-fagarine as the primary metabolite, reaching 448 mg/100 g DW. The optimal conditions for secondary metabolite accumulation in <i>R. corsica</i> cultures were a 5-week growth cycle and the LS 0.1/0.1 medium variant.https://www.mdpi.com/1420-3049/29/22/5261<i>Ruta corsica</i>endemicin vitro culturesfuranocoumarinsfuroquinoline alkaloidsHPLC analyses |
| spellingShingle | Agnieszka Szewczyk Monika Trepa Dominika Zych Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors Molecules <i>Ruta corsica</i> endemic in vitro cultures furanocoumarins furoquinoline alkaloids HPLC analyses |
| title | Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors |
| title_full | Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors |
| title_fullStr | Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors |
| title_full_unstemmed | Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors |
| title_short | Optimization of the Production of Secondary Metabolites from Furanocoumarin and Furoquinoline Alkaloid Groups in In Vitro <i>Ruta corsica</i> Cultures Grown in Temporary Immersion Bioreactors |
| title_sort | optimization of the production of secondary metabolites from furanocoumarin and furoquinoline alkaloid groups in in vitro i ruta corsica i cultures grown in temporary immersion bioreactors |
| topic | <i>Ruta corsica</i> endemic in vitro cultures furanocoumarins furoquinoline alkaloids HPLC analyses |
| url | https://www.mdpi.com/1420-3049/29/22/5261 |
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