Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment
IntroductionTumor tissues exhibit significantly lower oxygen partial pressure compared to normal tissues, leading to hypoxia in the tumor microenvironment and result in resistance to tumor treatments. Strategies to mitigate hypoxia include enhancing blood perfusion and oxygen supply, for example,by...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1502349/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543857598103552 |
|---|---|
| author | Yuyi Feng Danxia Qiu Yangcheng He Hai Jin Liping Chen Fen Xi Zhiwen Hu Yanlin Xie Yucai Li Minhua Lin Pengxiao Sun Yan He Jianhua Liu |
| author_facet | Yuyi Feng Danxia Qiu Yangcheng He Hai Jin Liping Chen Fen Xi Zhiwen Hu Yanlin Xie Yucai Li Minhua Lin Pengxiao Sun Yan He Jianhua Liu |
| author_sort | Yuyi Feng |
| collection | DOAJ |
| description | IntroductionTumor tissues exhibit significantly lower oxygen partial pressure compared to normal tissues, leading to hypoxia in the tumor microenvironment and result in resistance to tumor treatments. Strategies to mitigate hypoxia include enhancing blood perfusion and oxygen supply, for example,by decomposing hydrogen peroxide within the tumor. Improving hypoxia in the tumor microenvironment could potentially improve the efficacy of cancer treatments. Previous studies have demonstrated that ultrasound of appropriate intensity when combined with microbubbles, can improve tumor blood perfusion. However, its effects on tumor hypoxia remain unclear. This study aimed to assess the effects of low-frequency non-focused ultrasound combined with microbubbles at different intensities on tumor microenvironment hypoxia and to identify the optimal ultrasound parameters for alleviating tumor hypoxia.MethodRabbits with VX2 tumors received ultrasound and microbubble treatments at different acoustic pressures and pulse repetition frequencies. The changes in tumor tissue blood perfusion before and after treatment were observed by contrast enhanced ultrasound (CEUS). The changes in tumor tissue hypoxia before and after treatment were observed by measuring oxygen partial pressure directly with in tumor tissue and immunohistochemical staining for hypoxia-inducible factor-1α (HIF-1α).ResultsResults indicated that low frequency, non-focused ultrasound at 0.5 MPa/20 Hz and 0.5 MPa/40 Hz, when combined with microbubbles, could increase tumor tissue blood perfusion and improve the hypoxia in tumor tissues.DiscussionThis study provides a new method for improving hypoxia in the tumor microenvironment (TME) which could potentially improve the cancer treatments resistance. |
| format | Article |
| id | doaj-art-b05e6f66e4bb49f8b1470ec019ac65ba |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-b05e6f66e4bb49f8b1470ec019ac65ba2025-01-13T05:10:14ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15023491502349Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironmentYuyi FengDanxia QiuYangcheng HeHai JinLiping ChenFen XiZhiwen HuYanlin XieYucai LiMinhua LinPengxiao SunYan HeJianhua LiuIntroductionTumor tissues exhibit significantly lower oxygen partial pressure compared to normal tissues, leading to hypoxia in the tumor microenvironment and result in resistance to tumor treatments. Strategies to mitigate hypoxia include enhancing blood perfusion and oxygen supply, for example,by decomposing hydrogen peroxide within the tumor. Improving hypoxia in the tumor microenvironment could potentially improve the efficacy of cancer treatments. Previous studies have demonstrated that ultrasound of appropriate intensity when combined with microbubbles, can improve tumor blood perfusion. However, its effects on tumor hypoxia remain unclear. This study aimed to assess the effects of low-frequency non-focused ultrasound combined with microbubbles at different intensities on tumor microenvironment hypoxia and to identify the optimal ultrasound parameters for alleviating tumor hypoxia.MethodRabbits with VX2 tumors received ultrasound and microbubble treatments at different acoustic pressures and pulse repetition frequencies. The changes in tumor tissue blood perfusion before and after treatment were observed by contrast enhanced ultrasound (CEUS). The changes in tumor tissue hypoxia before and after treatment were observed by measuring oxygen partial pressure directly with in tumor tissue and immunohistochemical staining for hypoxia-inducible factor-1α (HIF-1α).ResultsResults indicated that low frequency, non-focused ultrasound at 0.5 MPa/20 Hz and 0.5 MPa/40 Hz, when combined with microbubbles, could increase tumor tissue blood perfusion and improve the hypoxia in tumor tissues.DiscussionThis study provides a new method for improving hypoxia in the tumor microenvironment (TME) which could potentially improve the cancer treatments resistance.https://www.frontiersin.org/articles/10.3389/fphar.2024.1502349/fulltumor hypoxic microenvironmentultrasoundmicrobubbleshypoxiaoxygen partial pressurecontrast enhance ultrasound |
| spellingShingle | Yuyi Feng Danxia Qiu Yangcheng He Hai Jin Liping Chen Fen Xi Zhiwen Hu Yanlin Xie Yucai Li Minhua Lin Pengxiao Sun Yan He Jianhua Liu Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment Frontiers in Pharmacology tumor hypoxic microenvironment ultrasound microbubbles hypoxia oxygen partial pressure contrast enhance ultrasound |
| title | Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| title_full | Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| title_fullStr | Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| title_full_unstemmed | Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| title_short | Effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| title_sort | effect of ultrasound combined with microbubbles therapy on tumor hypoxic microenvironment |
| topic | tumor hypoxic microenvironment ultrasound microbubbles hypoxia oxygen partial pressure contrast enhance ultrasound |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1502349/full |
| work_keys_str_mv | AT yuyifeng effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT danxiaqiu effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT yangchenghe effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT haijin effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT lipingchen effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT fenxi effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT zhiwenhu effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT yanlinxie effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT yucaili effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT minhualin effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT pengxiaosun effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT yanhe effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment AT jianhualiu effectofultrasoundcombinedwithmicrobubblestherapyontumorhypoxicmicroenvironment |