The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation
Abstract Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-82553-x |
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| author | Jillian M. Grantz Venkatesh P. Thirumalaikumar Amber H. Jannasch Chaylen Andolino Natnicha Taechachokevivat Lisa M. Avila-Granados Rafael C. Neves |
| author_facet | Jillian M. Grantz Venkatesh P. Thirumalaikumar Amber H. Jannasch Chaylen Andolino Natnicha Taechachokevivat Lisa M. Avila-Granados Rafael C. Neves |
| author_sort | Jillian M. Grantz |
| collection | DOAJ |
| description | Abstract Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows experiencing elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. Postpartum cows at 3 DIM with plasma Hp concentrations $$\:\ge\:$$ 0.50 g/L and no clinical disease were enrolled into the high-inflammation group (n = 8). Cows with plasma Hp concentrations $$\:\le\:$$ 0.1 g/L and no clinical disease were enrolled into the low-inflammation group (n = 8). Targeted lipidomic analysis revealed differences in the plasma oxylipin and endocannabinoid profile between high- and low-inflammation cows. Cows in the high-inflammation group had increased plasma concentrations of the oxylipins 9(S)-HpOTrE, 9(S)-HOTrE, 13(S)-HpOTrE, and 9,10-EpOME, and the endocannabinoid anandamide. In-depth proteomic analysis of platelets between the high- and low-inflammation groups revealed significant differences in protein categories related to platelet granule release and cellular iron uptake. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflammation groups, including proteins involved in autophagy and immune mediation. Together, our results indicate that cows experiencing an exacerbated systemic inflammatory response in the postpartum may have impaired disease resistance, and platelets could be contributors to their inflammatory state. |
| format | Article |
| id | doaj-art-af98bda7d42e4b10aebde77d2e541718 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-af98bda7d42e4b10aebde77d2e5417182024-12-29T12:23:02ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-82553-xThe platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammationJillian M. Grantz0Venkatesh P. Thirumalaikumar1Amber H. Jannasch2Chaylen Andolino3Natnicha Taechachokevivat4Lisa M. Avila-Granados5Rafael C. Neves6Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue UniversityPurdue Proteomics Facility, Bindley Bioscience Center, Purdue UniversityMetabolite Profiling Facility, Bindley Bioscience Center, Purdue UniversityPurdue Proteomics Facility, Bindley Bioscience Center, Purdue UniversityDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue UniversityDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue UniversityDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue UniversityAbstract Unregulated, systemic inflammation negatively impacts health and production in dairy cows. Soluble mediators and platelets have been studied for their expansive role in mediating inflammation. Our objectives were to compare the plasma oxylipin and endocannabinoid profiles, and the platelet and plasma proteomic profiles of healthy cows to cows experiencing elevated systemic inflammation as indicated by plasma haptoglobin (Hp) concentrations. Postpartum cows at 3 DIM with plasma Hp concentrations $$\:\ge\:$$ 0.50 g/L and no clinical disease were enrolled into the high-inflammation group (n = 8). Cows with plasma Hp concentrations $$\:\le\:$$ 0.1 g/L and no clinical disease were enrolled into the low-inflammation group (n = 8). Targeted lipidomic analysis revealed differences in the plasma oxylipin and endocannabinoid profile between high- and low-inflammation cows. Cows in the high-inflammation group had increased plasma concentrations of the oxylipins 9(S)-HpOTrE, 9(S)-HOTrE, 13(S)-HpOTrE, and 9,10-EpOME, and the endocannabinoid anandamide. In-depth proteomic analysis of platelets between the high- and low-inflammation groups revealed significant differences in protein categories related to platelet granule release and cellular iron uptake. Proteomic outputs from plasma revealed 24 proteins to be different between high and low-inflammation groups, including proteins involved in autophagy and immune mediation. Together, our results indicate that cows experiencing an exacerbated systemic inflammatory response in the postpartum may have impaired disease resistance, and platelets could be contributors to their inflammatory state.https://doi.org/10.1038/s41598-024-82553-xSystemic inflammationHaptoglobinPlateletOxylipinEndocannabinoid |
| spellingShingle | Jillian M. Grantz Venkatesh P. Thirumalaikumar Amber H. Jannasch Chaylen Andolino Natnicha Taechachokevivat Lisa M. Avila-Granados Rafael C. Neves The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation Scientific Reports Systemic inflammation Haptoglobin Platelet Oxylipin Endocannabinoid |
| title | The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| title_full | The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| title_fullStr | The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| title_full_unstemmed | The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| title_short | The platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| title_sort | platelet and plasma proteome and targeted lipidome in postpartum dairy cows with elevated systemic inflammation |
| topic | Systemic inflammation Haptoglobin Platelet Oxylipin Endocannabinoid |
| url | https://doi.org/10.1038/s41598-024-82553-x |
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