Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation
Bronchospheres have emerged as a promising in vitro model toward probing questions on organ development and disease. Several organoid models, including from airway (e.g., bronchial, tracheal) cells, require three‐dimensional (3D) Matrigel, a complex mouse tumor‐derived matrix that typically leads to...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-01-01
|
| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202300110 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556374693085184 |
|---|---|
| author | Madeline K. Eiken Justin E. Levine Shinyeong Lee Samantha Lukpat Eleanor M. Plaster Vikram Bala Jason R. Spence Claudia Loebel |
| author_facet | Madeline K. Eiken Justin E. Levine Shinyeong Lee Samantha Lukpat Eleanor M. Plaster Vikram Bala Jason R. Spence Claudia Loebel |
| author_sort | Madeline K. Eiken |
| collection | DOAJ |
| description | Bronchospheres have emerged as a promising in vitro model toward probing questions on organ development and disease. Several organoid models, including from airway (e.g., bronchial, tracheal) cells, require three‐dimensional (3D) Matrigel, a complex mouse tumor‐derived matrix that typically leads to heterogeneous size and structures. Synthetic and naturally derived polymeric hydrogels show increased opportunities as an alternative to Matrigel culture. In addition, recent advances in hydrogel‐based microcavities (i.e., microwells) have shown improved control over organoid size, structure, and composition. Here, we build upon this approach and describe the fabrication and characterization of microwell hydrogels based on other polymers, including diacrylated poly(ethylene glycol), agarose, methacrylated gelatin, and norbornene‐modified hyaluronic acid. Using these microwell hydrogels, human bronchial epithelial cells and lung fibroblasts readily assemble into viable cyst‐like bronchospheres. The study shows that the cellular composition regulates the formation and structure of the bronchosphere which also depends on the type and adhesiveness of the hydrogel. Furthermore, both hydrogel type and cellular composition influence the amount and composition of deposited extracellular matrix within the microwells. This hydrogel fabrication platform provides an accessible in vitro culture platform for the formation and growth of bronchospheres which can be extended to the culture of other organoid systems. |
| format | Article |
| id | doaj-art-af81a40781c04fde9c2fb2a113403edc |
| institution | Kabale University |
| issn | 2699-9307 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj-art-af81a40781c04fde9c2fb2a113403edc2025-01-07T09:13:09ZengWiley-VCHAdvanced NanoBiomed Research2699-93072025-01-0151n/an/a10.1002/anbr.202300110Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere FormationMadeline K. Eiken0Justin E. Levine1Shinyeong Lee2Samantha Lukpat3Eleanor M. Plaster4Vikram Bala5Jason R. Spence6Claudia Loebel7Department of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USADepartment of Biomedical Engineering University of Michigan Carl A. Gerstacker Building 2200 Bonisteel Blvd Ann Arbor MI 48109 USABronchospheres have emerged as a promising in vitro model toward probing questions on organ development and disease. Several organoid models, including from airway (e.g., bronchial, tracheal) cells, require three‐dimensional (3D) Matrigel, a complex mouse tumor‐derived matrix that typically leads to heterogeneous size and structures. Synthetic and naturally derived polymeric hydrogels show increased opportunities as an alternative to Matrigel culture. In addition, recent advances in hydrogel‐based microcavities (i.e., microwells) have shown improved control over organoid size, structure, and composition. Here, we build upon this approach and describe the fabrication and characterization of microwell hydrogels based on other polymers, including diacrylated poly(ethylene glycol), agarose, methacrylated gelatin, and norbornene‐modified hyaluronic acid. Using these microwell hydrogels, human bronchial epithelial cells and lung fibroblasts readily assemble into viable cyst‐like bronchospheres. The study shows that the cellular composition regulates the formation and structure of the bronchosphere which also depends on the type and adhesiveness of the hydrogel. Furthermore, both hydrogel type and cellular composition influence the amount and composition of deposited extracellular matrix within the microwells. This hydrogel fabrication platform provides an accessible in vitro culture platform for the formation and growth of bronchospheres which can be extended to the culture of other organoid systems.https://doi.org/10.1002/anbr.202300110bronchosphereshydrogels for cell culturemicrowells |
| spellingShingle | Madeline K. Eiken Justin E. Levine Shinyeong Lee Samantha Lukpat Eleanor M. Plaster Vikram Bala Jason R. Spence Claudia Loebel Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation Advanced NanoBiomed Research bronchospheres hydrogels for cell culture microwells |
| title | Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation |
| title_full | Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation |
| title_fullStr | Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation |
| title_full_unstemmed | Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation |
| title_short | Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation |
| title_sort | polymer design of microwell hydrogels influences epithelial mesenchymal interactions during human bronchosphere formation |
| topic | bronchospheres hydrogels for cell culture microwells |
| url | https://doi.org/10.1002/anbr.202300110 |
| work_keys_str_mv | AT madelinekeiken polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT justinelevine polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT shinyeonglee polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT samanthalukpat polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT eleanormplaster polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT vikrambala polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT jasonrspence polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation AT claudialoebel polymerdesignofmicrowellhydrogelsinfluencesepithelialmesenchymalinteractionsduringhumanbronchosphereformation |