Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma
This study explored the relationships between sperm quality and the activities of antioxidant enzymes (superoxide dismutase, SOD, and glutathione peroxidase, GPx) in sperm (S) and seminal plasma (SP) of Asian elephants, as well as the potential protective role of glutamine in semen extenders during...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Theriogenology Wild |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773093X24000345 |
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| author | Yuqing Yang Junpen Suwimonteerabutr Taweepoke Angkawanish Nicole Sirisopit Mehl Patharapol Piamsomboon Nuttawadee Ngamlertwong Padet Tummaruk Pakpoom Navanukraw Phakjira Sanguansook Kaywalee Chatdarong |
| author_facet | Yuqing Yang Junpen Suwimonteerabutr Taweepoke Angkawanish Nicole Sirisopit Mehl Patharapol Piamsomboon Nuttawadee Ngamlertwong Padet Tummaruk Pakpoom Navanukraw Phakjira Sanguansook Kaywalee Chatdarong |
| author_sort | Yuqing Yang |
| collection | DOAJ |
| description | This study explored the relationships between sperm quality and the activities of antioxidant enzymes (superoxide dismutase, SOD, and glutathione peroxidase, GPx) in sperm (S) and seminal plasma (SP) of Asian elephants, as well as the potential protective role of glutamine in semen extenders during chilled storage. In experiment 1, twenty ejaculates from 11 bulls were analyzed for percentages (%) of sperm motility (%MOT), viability (%VIA), and activities of SOD and GPx. A significant negative correlation was found between %VIA and SP-SOD (r = − 0.71), and positive correlations between %MOT and both S-GPx (r = 0.49) and SP-GPx (r = 0.57). Following these findings, glutamine, a precursor of glutathione and semen antioxidant was introduced in Experiment 2. Each ejaculate (in a total of 8 ejaculates obtained from four bulls) was diluted in Tris-fructose- (EYT) and Tris-glucose-egg yolk extender (TCG), respectively, supplemented with 0 (control), 20 and 40 mM glutamine and stored at 4 °C. Sperm parameters including %MOT, %VIA, functional membrane integrity (%sHOST), and DNA integrity (%DNA) were assessed after 24 and 48 h of storage. Results demonstrated that glutamine did not significantly affect %VIA or %DNA. A significant reduction in %MOT was observed at 24 h, but 20 mM glutamine added in extenders reversed this decline at 48 h. A 40 mM addition of glutamine in TCG markedly reduced %sHOST at 24 h. In conclusion, SOD and GPx activities in the sperm and seminal plasma are indicative of sperm quality. During chilling, using a higher level of glutamine may damage the spermatozoa of Asian elephants. |
| format | Article |
| id | doaj-art-af27ffa2cd81476bbd5b9f306d80f6f3 |
| institution | Kabale University |
| issn | 2773-093X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Theriogenology Wild |
| spelling | doaj-art-af27ffa2cd81476bbd5b9f306d80f6f32024-12-12T05:24:47ZengElsevierTheriogenology Wild2773-093X2024-01-015100103Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasmaYuqing Yang0Junpen Suwimonteerabutr1Taweepoke Angkawanish2Nicole Sirisopit Mehl3Patharapol Piamsomboon4Nuttawadee Ngamlertwong5Padet Tummaruk6Pakpoom Navanukraw7Phakjira Sanguansook8Kaywalee Chatdarong9Research Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandThe Thai Elephant Conservation Center, National Elephant Institute of Thailand, The Forest Industry Organization, Lampang 52190, ThailandDepartment of Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandFaculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, ThailandResearch Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Unit of Obstetrics and Reproduction in Animals, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand; Corresponding author.This study explored the relationships between sperm quality and the activities of antioxidant enzymes (superoxide dismutase, SOD, and glutathione peroxidase, GPx) in sperm (S) and seminal plasma (SP) of Asian elephants, as well as the potential protective role of glutamine in semen extenders during chilled storage. In experiment 1, twenty ejaculates from 11 bulls were analyzed for percentages (%) of sperm motility (%MOT), viability (%VIA), and activities of SOD and GPx. A significant negative correlation was found between %VIA and SP-SOD (r = − 0.71), and positive correlations between %MOT and both S-GPx (r = 0.49) and SP-GPx (r = 0.57). Following these findings, glutamine, a precursor of glutathione and semen antioxidant was introduced in Experiment 2. Each ejaculate (in a total of 8 ejaculates obtained from four bulls) was diluted in Tris-fructose- (EYT) and Tris-glucose-egg yolk extender (TCG), respectively, supplemented with 0 (control), 20 and 40 mM glutamine and stored at 4 °C. Sperm parameters including %MOT, %VIA, functional membrane integrity (%sHOST), and DNA integrity (%DNA) were assessed after 24 and 48 h of storage. Results demonstrated that glutamine did not significantly affect %VIA or %DNA. A significant reduction in %MOT was observed at 24 h, but 20 mM glutamine added in extenders reversed this decline at 48 h. A 40 mM addition of glutamine in TCG markedly reduced %sHOST at 24 h. In conclusion, SOD and GPx activities in the sperm and seminal plasma are indicative of sperm quality. During chilling, using a higher level of glutamine may damage the spermatozoa of Asian elephants.http://www.sciencedirect.com/science/article/pii/S2773093X24000345Chilled semenGlutamineGlutathione peroxidaseSuperoxide dismutase |
| spellingShingle | Yuqing Yang Junpen Suwimonteerabutr Taweepoke Angkawanish Nicole Sirisopit Mehl Patharapol Piamsomboon Nuttawadee Ngamlertwong Padet Tummaruk Pakpoom Navanukraw Phakjira Sanguansook Kaywalee Chatdarong Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma Theriogenology Wild Chilled semen Glutamine Glutathione peroxidase Superoxide dismutase |
| title | Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma |
| title_full | Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma |
| title_fullStr | Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma |
| title_full_unstemmed | Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma |
| title_short | Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma |
| title_sort | exploring enzymatic antioxidant activities in asian elephant elephas maximus sperm and seminal plasma |
| topic | Chilled semen Glutamine Glutathione peroxidase Superoxide dismutase |
| url | http://www.sciencedirect.com/science/article/pii/S2773093X24000345 |
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