Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils
Fortification of edible oil with retinyl palmitate (vitamin A) is a significant advancement to fulfill the nutritional deficiency of major population. The growing interest in the enrichment of foods warrants a simple and accurate method to analyze fortified products. This study developed an easy and...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-09-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2022.2151618 |
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| author | Rakibul Hasan Rokeya Begum A.K. Obidul Huq Nannur Rahman Shamoli Akter Shahinul Haque Khan Faridul Islam |
| author_facet | Rakibul Hasan Rokeya Begum A.K. Obidul Huq Nannur Rahman Shamoli Akter Shahinul Haque Khan Faridul Islam |
| author_sort | Rakibul Hasan |
| collection | DOAJ |
| description | Fortification of edible oil with retinyl palmitate (vitamin A) is a significant advancement to fulfill the nutritional deficiency of major population. The growing interest in the enrichment of foods warrants a simple and accurate method to analyze fortified products. This study developed an easy and reliable strategy based on normal-phase HPLC analysis to determine the edible oil’s retinyl palmitate. We also validated the method according to the International Conference on Harmonization (ICH) guidelines. The analysis was performed with a DB silica column (250 cm × 4.6 mm; 5 μm), with a mobile phase composed of n-heptane and isopropyl alcohol at a ratio of 75:25 v/v and a flow rate of 1.0 ml/min. The detection was performed at a wavelength of 326 nm, and the retention time of vitamin A was around 8 min, with a total run time of 10 min. The calibration plot gave a linear relationship (R2 = 0.9998) over the concentration range of 3.93–63 μg/ml. The LOD and LOQ were 0.029 and 0.096 μg/ml, respectively. The accuracy of the proposed method was determined by recovery studies and was found with a mean recovery of >95%. RSD% of the determination of precision was <2%. The results of robustness and solutions stability studies were within acceptable limits. The proposed method showed excellent linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptable criteria. |
| format | Article |
| id | doaj-art-aeddc99717a84c69a6aa54b3ddd73673 |
| institution | Kabale University |
| issn | 1094-2912 1532-2386 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj-art-aeddc99717a84c69a6aa54b3ddd736732025-01-02T10:41:34ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-09-01261243710.1080/10942912.2022.2151618Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oilsRakibul Hasan0Rokeya Begum1A.K. Obidul Huq2Nannur Rahman3Shamoli Akter4Shahinul Haque Khan5Faridul Islam6Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, BangladeshDepartment of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, BangladeshDepartment of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, BangladeshDepartment of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, BangladeshDepartment of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, BangladeshDepartment of Chemistry, Bangladesh University of Health Sciences (BUHS), Dhaka, BangladeshInstitute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, BangladeshFortification of edible oil with retinyl palmitate (vitamin A) is a significant advancement to fulfill the nutritional deficiency of major population. The growing interest in the enrichment of foods warrants a simple and accurate method to analyze fortified products. This study developed an easy and reliable strategy based on normal-phase HPLC analysis to determine the edible oil’s retinyl palmitate. We also validated the method according to the International Conference on Harmonization (ICH) guidelines. The analysis was performed with a DB silica column (250 cm × 4.6 mm; 5 μm), with a mobile phase composed of n-heptane and isopropyl alcohol at a ratio of 75:25 v/v and a flow rate of 1.0 ml/min. The detection was performed at a wavelength of 326 nm, and the retention time of vitamin A was around 8 min, with a total run time of 10 min. The calibration plot gave a linear relationship (R2 = 0.9998) over the concentration range of 3.93–63 μg/ml. The LOD and LOQ were 0.029 and 0.096 μg/ml, respectively. The accuracy of the proposed method was determined by recovery studies and was found with a mean recovery of >95%. RSD% of the determination of precision was <2%. The results of robustness and solutions stability studies were within acceptable limits. The proposed method showed excellent linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptable criteria.https://www.tandfonline.com/doi/10.1080/10942912.2022.2151618Normal-phase HPLCFortified edible oilsRetinyl palmitateValidationRecovery |
| spellingShingle | Rakibul Hasan Rokeya Begum A.K. Obidul Huq Nannur Rahman Shamoli Akter Shahinul Haque Khan Faridul Islam Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils International Journal of Food Properties Normal-phase HPLC Fortified edible oils Retinyl palmitate Validation Recovery |
| title | Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils |
| title_full | Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils |
| title_fullStr | Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils |
| title_full_unstemmed | Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils |
| title_short | Development of normal phase HPLC based method for the determination of retinyl palmitate in fortified edible oils |
| title_sort | development of normal phase hplc based method for the determination of retinyl palmitate in fortified edible oils |
| topic | Normal-phase HPLC Fortified edible oils Retinyl palmitate Validation Recovery |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2022.2151618 |
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