Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study

To investigate the correlation between blood stasis syndrome and arteriopathy in immunoglobulin A nephropathy (IgAN). Wall thickness/outer vessel diameter, intimal thickness/outer vessel diameter, and medial thickness/outer vessel diameter were measured using ImageJ software. Vascular endothelial-de...

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Main Authors: Wang Ruiqi, Tian Yun
Format: Article
Language:English
Published: De Gruyter 2024-12-01
Series:Open Medicine
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Online Access:https://doi.org/10.1515/med-2024-1042
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author Wang Ruiqi
Tian Yun
author_facet Wang Ruiqi
Tian Yun
author_sort Wang Ruiqi
collection DOAJ
description To investigate the correlation between blood stasis syndrome and arteriopathy in immunoglobulin A nephropathy (IgAN). Wall thickness/outer vessel diameter, intimal thickness/outer vessel diameter, and medial thickness/outer vessel diameter were measured using ImageJ software. Vascular endothelial-derived growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase (ERK) 1/2, and nuclear factor kappa B (NF-κB) were detected by immunohistochemical staining. Twenty-four-hour urine protein quantification, serum creatinine, urea nitrogen, and uric acid were collected. Blood stasis syndrome and vessel scores were calculated based on Katafuchi’s grade. Intimal thickness/outer vessel diameter (0.2725 ± 0.0932 μm), medial thickness/outer vessel diameter (0.2747 ± 0.1139 μm), and wall thickness/outer vessel diameter (0.6136 ± 0.1120 μm) were the largest in IgAN with arteriopathy group. VEGF (0.35 ± 0.90), MMP-9 (0.38 ± 0.12), PCNA (0.43 ± 0.12), ERK1/2 (0.31 ± 0.11), and NF-κB (0.37 ± 0.14) were the highest in IgAN with arteriopathy group. Intimal thickening of IgAN was moderately positively correlated with VEGF, MMP-9, PCNA, ERK1/2, and NF-κB (0.5 < r < 0.8). Medial thickening of IgAN was moderately positively correlated with PCNA and NF-κB (0.5 < r < 0.8). Wall thickening of IgAN was lowly positively correlated with VEGF and MMP-9 (0.3 < r < 0.5). Blood stasis syndrome score was associated with vessel score in IgAN with arteriopathy (P < 0.05). Blood stasis syndrome score can assess the degree of pathological changes.
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spelling doaj-art-aec9c1f6314c42d69d06bcd318804f132024-12-10T07:31:39ZengDe GruyterOpen Medicine2391-54632024-12-011912785910.1515/med-2024-1042Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort studyWang Ruiqi0Tian Yun1Department of Traditional Chinese Medicine, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi’an, 710003, ChinaDepartment of Nephrology, Shaanxi Provincial Hospital of Traditional Chinese Medicine, No. 4, Xihuamen, Xi’an, Shaanxi, 710003, ChinaTo investigate the correlation between blood stasis syndrome and arteriopathy in immunoglobulin A nephropathy (IgAN). Wall thickness/outer vessel diameter, intimal thickness/outer vessel diameter, and medial thickness/outer vessel diameter were measured using ImageJ software. Vascular endothelial-derived growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase (ERK) 1/2, and nuclear factor kappa B (NF-κB) were detected by immunohistochemical staining. Twenty-four-hour urine protein quantification, serum creatinine, urea nitrogen, and uric acid were collected. Blood stasis syndrome and vessel scores were calculated based on Katafuchi’s grade. Intimal thickness/outer vessel diameter (0.2725 ± 0.0932 μm), medial thickness/outer vessel diameter (0.2747 ± 0.1139 μm), and wall thickness/outer vessel diameter (0.6136 ± 0.1120 μm) were the largest in IgAN with arteriopathy group. VEGF (0.35 ± 0.90), MMP-9 (0.38 ± 0.12), PCNA (0.43 ± 0.12), ERK1/2 (0.31 ± 0.11), and NF-κB (0.37 ± 0.14) were the highest in IgAN with arteriopathy group. Intimal thickening of IgAN was moderately positively correlated with VEGF, MMP-9, PCNA, ERK1/2, and NF-κB (0.5 < r < 0.8). Medial thickening of IgAN was moderately positively correlated with PCNA and NF-κB (0.5 < r < 0.8). Wall thickening of IgAN was lowly positively correlated with VEGF and MMP-9 (0.3 < r < 0.5). Blood stasis syndrome score was associated with vessel score in IgAN with arteriopathy (P < 0.05). Blood stasis syndrome score can assess the degree of pathological changes.https://doi.org/10.1515/med-2024-1042iganarteriopathycorrelationblood stasis syndrome
spellingShingle Wang Ruiqi
Tian Yun
Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
Open Medicine
igan
arteriopathy
correlation
blood stasis syndrome
title Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
title_full Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
title_fullStr Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
title_full_unstemmed Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
title_short Correlation between the mechanism of arteriopathy in IgA nephropathy and blood stasis syndrome: A cohort study
title_sort correlation between the mechanism of arteriopathy in iga nephropathy and blood stasis syndrome a cohort study
topic igan
arteriopathy
correlation
blood stasis syndrome
url https://doi.org/10.1515/med-2024-1042
work_keys_str_mv AT wangruiqi correlationbetweenthemechanismofarteriopathyiniganephropathyandbloodstasissyndromeacohortstudy
AT tianyun correlationbetweenthemechanismofarteriopathyiniganephropathyandbloodstasissyndromeacohortstudy