Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation
Cellulose is a complex polysaccharide composed of β 1, 4 glycosidic linkages and these linkages are broken down by a complex enzyme system called cellulase. Cellulase is an enzyme complex associated with β 1, 4 endoglucanase, β 1, 4 exoglucanase, and β glucosidase. The fungal strains were isolated f...
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Elsevier
2024-12-01
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| author | H.K. Narendra Kumar N. Chandra Mohana Jayarama Reddy M.R. Abhilash S. Satish |
| author_facet | H.K. Narendra Kumar N. Chandra Mohana Jayarama Reddy M.R. Abhilash S. Satish |
| author_sort | H.K. Narendra Kumar |
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| description | Cellulose is a complex polysaccharide composed of β 1, 4 glycosidic linkages and these linkages are broken down by a complex enzyme system called cellulase. Cellulase is an enzyme complex associated with β 1, 4 endoglucanase, β 1, 4 exoglucanase, and β glucosidase. The fungal strains were isolated from decomposed humus soil and screened for the best cellulolytic activity. Thirty-two isolates were screened among these; three were selected based on their morphological characters and hydrolytic capacity, viz, Cladosporium oxysporum N5, Aspergillus sigurros N6, and Cladosporium cladosporioides N12. Among these fungi, Cladosporium oxysporum N5 shows potential hydrolytic activity and was selected for optimization studies and evaluation of their cellulolytic capability. Cellulolytic strain Cladosporium oxysporum N5 produced maximum amount of cellulase enzyme at 5 days of incubation at 30 ℃ and pH 7, viz, β 1, 4 endoglucanase (167.83± U/ml), β 1, 4 exoglucanase (29.04± U/ml), and β glucosidase (34.07± U/ml). SEM coupled Energy dispersive X-ray and FTIR spectroscopy methods revealed the cellulose degradation efficiency of fungal cellulase by microscopic, elemental percentage, and functional group, respectively. An affordable and eco-friendly fungal-based cellulase that will boost industrially important commodity production such as food, beverages, pharmaceuticals, fertilisers, and biofuels. |
| format | Article |
| id | doaj-art-aec97d1f7d104cf7922c49be604d4208 |
| institution | Kabale University |
| issn | 2950-1946 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | The Microbe |
| spelling | doaj-art-aec97d1f7d104cf7922c49be604d42082024-12-18T08:55:43ZengElsevierThe Microbe2950-19462024-12-015100183Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradationH.K. Narendra Kumar0N. Chandra Mohana1Jayarama Reddy2M.R. Abhilash3S. Satish4Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, IndiaDepartment of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, IndiaDepartment of Botany, St. Joseph’s University, 36 Lalbagh Road, Bengaluru, Karnataka 560 027, India; Corresponding authors.Department of Studies in Environmental Science, University of Mysore, Mysore, Karnataka 570006, IndiaDepartment of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, India; Corresponding authors.Cellulose is a complex polysaccharide composed of β 1, 4 glycosidic linkages and these linkages are broken down by a complex enzyme system called cellulase. Cellulase is an enzyme complex associated with β 1, 4 endoglucanase, β 1, 4 exoglucanase, and β glucosidase. The fungal strains were isolated from decomposed humus soil and screened for the best cellulolytic activity. Thirty-two isolates were screened among these; three were selected based on their morphological characters and hydrolytic capacity, viz, Cladosporium oxysporum N5, Aspergillus sigurros N6, and Cladosporium cladosporioides N12. Among these fungi, Cladosporium oxysporum N5 shows potential hydrolytic activity and was selected for optimization studies and evaluation of their cellulolytic capability. Cellulolytic strain Cladosporium oxysporum N5 produced maximum amount of cellulase enzyme at 5 days of incubation at 30 ℃ and pH 7, viz, β 1, 4 endoglucanase (167.83± U/ml), β 1, 4 exoglucanase (29.04± U/ml), and β glucosidase (34.07± U/ml). SEM coupled Energy dispersive X-ray and FTIR spectroscopy methods revealed the cellulose degradation efficiency of fungal cellulase by microscopic, elemental percentage, and functional group, respectively. An affordable and eco-friendly fungal-based cellulase that will boost industrially important commodity production such as food, beverages, pharmaceuticals, fertilisers, and biofuels.http://www.sciencedirect.com/science/article/pii/S295019462400150XCelluloseCarboxymethyl celluloseCladosporium oxysporumOptimizationSEM-EDXFTIR |
| spellingShingle | H.K. Narendra Kumar N. Chandra Mohana Jayarama Reddy M.R. Abhilash S. Satish Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation The Microbe Cellulose Carboxymethyl cellulose Cladosporium oxysporum Optimization SEM-EDX FTIR |
| title | Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| title_full | Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| title_fullStr | Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| title_full_unstemmed | Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| title_short | Unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| title_sort | unravelling of cellulolytic fungal consortium from humus soil for efficient lignocellulosic waste degradation |
| topic | Cellulose Carboxymethyl cellulose Cladosporium oxysporum Optimization SEM-EDX FTIR |
| url | http://www.sciencedirect.com/science/article/pii/S295019462400150X |
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