<i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice
<i>Duguetia furfuracea</i>, “araticum-seco”, is known to contain several bioactive compounds that can mitigate oxidative stress and act on the central nervous system (CNS). This effect is partly attributed to its potent antioxidant and acetylcholinesterase (AChE) inhibitors. In this stud...
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2024-11-01
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| author | Maiara Fava de Souza Jéssica Maurino dos Santos Sidney Mariano dos Santos Pedro Cruz de Oliveira Junior Janaine Alberto Marangoni Faoro Arielle Cristina Arena Lívia Trippe Nagaoka Gisele de Freitas Gauze Rodrigo Juliano Oliveira Matheus Henrique Barbim Rech Rosilda Mara Mussury Franco Silva Anelise Samara Nazari Formagio |
| author_facet | Maiara Fava de Souza Jéssica Maurino dos Santos Sidney Mariano dos Santos Pedro Cruz de Oliveira Junior Janaine Alberto Marangoni Faoro Arielle Cristina Arena Lívia Trippe Nagaoka Gisele de Freitas Gauze Rodrigo Juliano Oliveira Matheus Henrique Barbim Rech Rosilda Mara Mussury Franco Silva Anelise Samara Nazari Formagio |
| author_sort | Maiara Fava de Souza |
| collection | DOAJ |
| description | <i>Duguetia furfuracea</i>, “araticum-seco”, is known to contain several bioactive compounds that can mitigate oxidative stress and act on the central nervous system (CNS). This effect is partly attributed to its potent antioxidant and acetylcholinesterase (AChE) inhibitors. In this study, the effects were explored of the methanolic extract (MEDF) and alkaloid fraction (AFDF) of <i>D. furfuracea</i> (leaves) on cognitive behaviors in male mice with scopolamine (Scop)-induced cognitive impairment and biochemical parameters. Additionally, anxiolytic behavior, subacute toxicity, molecular docking and antioxidant activity were reported. MEDF (30, 100 or 300 mg/kg) or AFDF (30 mg/kg) were orally administered for 16 days and Scop (intraperitoneally, i.p.) between days 11 and 16. The anxiolytic behavior (open field test and marble burying) in healthy mice, and the Scop-induced memory impairment (object recognition test and Morris water maze (MWM)) were assessed, and the biochemical parameters (malondialdehyde (MDA) and AChE levels) were measured after euthanasia. The subacute toxicological impact of MEDF was assessed in female Swiss mice for 28 days. MEDF and AFDF were available for the DPPH, ABTS and β-carotene/linoleic acid models. The results revealed that MEDF and AFDF exhibit anxiolytic effects and significantly alleviated Sco-induced memory impairment, inhibited AChE in the cortex (40%) and MDA (51.51%) levels. Reticuline was reported in AFDF and molecular coupling with AChE involves link-type hydrogen bonds and van der Waals interactions. MEDF exhibited antioxidant capacity (DPPH, IC<sub>50</sub> = 18.10 ± 1.70 µg/mL; ABTS, IC<sub>50</sub> = 10.41 ± 1.69 µg/mL). MEDF did not reveal signs of toxicity. In conclusion, <i>D. furfuracea</i> shows promise in mitigating scopolamine-induced memory deficits, potentially because it inhibits AChE activity, reduces MDA levels, and enhances antioxidant activities. |
| format | Article |
| id | doaj-art-ae9dc303ef1346edbb8954c1a8544361 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-ae9dc303ef1346edbb8954c1a85443612024-12-27T14:11:54ZengMDPI AGBiology2079-77372024-11-01131298110.3390/biology13120981<i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in MiceMaiara Fava de Souza0Jéssica Maurino dos Santos1Sidney Mariano dos Santos2Pedro Cruz de Oliveira Junior3Janaine Alberto Marangoni Faoro4Arielle Cristina Arena5Lívia Trippe Nagaoka6Gisele de Freitas Gauze7Rodrigo Juliano Oliveira8Matheus Henrique Barbim Rech9Rosilda Mara Mussury Franco Silva10Anelise Samara Nazari Formagio11College of Health Science, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilCollege of Health Science, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilCollege of Health Science, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilCollege of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilCollege of Health Science, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilInstitute of Biosciences of Botucatu, Department of Structural and Functional Biology, University Estadual Paulista—Botucatu (UNESP), São Paulo 18618-689, SP, BrazilInstitute of Biosciences of Botucatu, Department of Structural and Functional Biology, University Estadual Paulista—Botucatu (UNESP), São Paulo 18618-689, SP, BrazilDepartment of Chemistry, State University of Maringá UEM, Maringá 87020-900, PR, BrazilCentro de Estudos em Células-Tronco, Terapia Celular e Genética Toxicológica (CeTroGen), Faculdade de Medicina (FAMED), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande 79070-900, MS, BrazilCentro de Estudos em Células-Tronco, Terapia Celular e Genética Toxicológica (CeTroGen), Faculdade de Medicina (FAMED), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande 79070-900, MS, BrazilCollege of Biological and Environmental Sciences, Federal University of Grande Dourados, Dourados 79804-970, MS, BrazilCollege of Health Science, Federal University of Grande Dourados, Dourados 79804-970, MS, Brazil<i>Duguetia furfuracea</i>, “araticum-seco”, is known to contain several bioactive compounds that can mitigate oxidative stress and act on the central nervous system (CNS). This effect is partly attributed to its potent antioxidant and acetylcholinesterase (AChE) inhibitors. In this study, the effects were explored of the methanolic extract (MEDF) and alkaloid fraction (AFDF) of <i>D. furfuracea</i> (leaves) on cognitive behaviors in male mice with scopolamine (Scop)-induced cognitive impairment and biochemical parameters. Additionally, anxiolytic behavior, subacute toxicity, molecular docking and antioxidant activity were reported. MEDF (30, 100 or 300 mg/kg) or AFDF (30 mg/kg) were orally administered for 16 days and Scop (intraperitoneally, i.p.) between days 11 and 16. The anxiolytic behavior (open field test and marble burying) in healthy mice, and the Scop-induced memory impairment (object recognition test and Morris water maze (MWM)) were assessed, and the biochemical parameters (malondialdehyde (MDA) and AChE levels) were measured after euthanasia. The subacute toxicological impact of MEDF was assessed in female Swiss mice for 28 days. MEDF and AFDF were available for the DPPH, ABTS and β-carotene/linoleic acid models. The results revealed that MEDF and AFDF exhibit anxiolytic effects and significantly alleviated Sco-induced memory impairment, inhibited AChE in the cortex (40%) and MDA (51.51%) levels. Reticuline was reported in AFDF and molecular coupling with AChE involves link-type hydrogen bonds and van der Waals interactions. MEDF exhibited antioxidant capacity (DPPH, IC<sub>50</sub> = 18.10 ± 1.70 µg/mL; ABTS, IC<sub>50</sub> = 10.41 ± 1.69 µg/mL). MEDF did not reveal signs of toxicity. In conclusion, <i>D. furfuracea</i> shows promise in mitigating scopolamine-induced memory deficits, potentially because it inhibits AChE activity, reduces MDA levels, and enhances antioxidant activities.https://www.mdpi.com/2079-7737/13/12/981“araticum seco”AChEanxietydementiascopolamine |
| spellingShingle | Maiara Fava de Souza Jéssica Maurino dos Santos Sidney Mariano dos Santos Pedro Cruz de Oliveira Junior Janaine Alberto Marangoni Faoro Arielle Cristina Arena Lívia Trippe Nagaoka Gisele de Freitas Gauze Rodrigo Juliano Oliveira Matheus Henrique Barbim Rech Rosilda Mara Mussury Franco Silva Anelise Samara Nazari Formagio <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice Biology “araticum seco” AChE anxiety dementia scopolamine |
| title | <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice |
| title_full | <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice |
| title_fullStr | <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice |
| title_full_unstemmed | <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice |
| title_short | <i>Duguetia furfuracea</i> (A.ST. Hil.) Saff.: Neuroprotective Effect on Chemically Induced Amnesia, Anxiolytic Effects and Preclinical Safety Evaluation in Mice |
| title_sort | i duguetia furfuracea i a st hil saff neuroprotective effect on chemically induced amnesia anxiolytic effects and preclinical safety evaluation in mice |
| topic | “araticum seco” AChE anxiety dementia scopolamine |
| url | https://www.mdpi.com/2079-7737/13/12/981 |
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