The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis
Lung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2024.2347377 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846139358690672640 |
|---|---|
| author | Taotao Yue Jinpeng Wang Fang Liu Pengtao Gong Jianhua Li Xichen Zhang Nan Zhang |
| author_facet | Taotao Yue Jinpeng Wang Fang Liu Pengtao Gong Jianhua Li Xichen Zhang Nan Zhang |
| author_sort | Taotao Yue |
| collection | DOAJ |
| description | Lung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between A549 cells and Trichinella spiralis (T. spiralis). Our data showed that anti-Ts7TMR scFv could inhibit lung cancer growth in a dose-dependent manner, with a tumour inhibition rate of 59.1%. HE staining did not reveal any obvious tissue damage. Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer. |
| format | Article |
| id | doaj-art-ae2aabe187b741fe8409b557bab6839b |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-ae2aabe187b741fe8409b557bab6839b2024-12-06T10:48:00ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152130030810.1080/21691401.2024.2347377The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralisTaotao Yue0Jinpeng Wang1Fang Liu2Pengtao Gong3Jianhua Li4Xichen Zhang5Nan Zhang6Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaFirst Hospital, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, ChinaLung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between A549 cells and Trichinella spiralis (T. spiralis). Our data showed that anti-Ts7TMR scFv could inhibit lung cancer growth in a dose-dependent manner, with a tumour inhibition rate of 59.1%. HE staining did not reveal any obvious tissue damage. Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer.https://www.tandfonline.com/doi/10.1080/21691401.2024.2347377Trichinella spiralisA549 lung cancerassociated antigensTs7TMRscFv |
| spellingShingle | Taotao Yue Jinpeng Wang Fang Liu Pengtao Gong Jianhua Li Xichen Zhang Nan Zhang The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis Artificial Cells, Nanomedicine, and Biotechnology Trichinella spiralis A549 lung cancer associated antigens Ts7TMR scFv |
| title | The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis |
| title_full | The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis |
| title_fullStr | The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis |
| title_full_unstemmed | The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis |
| title_short | The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis |
| title_sort | effects of anti lung cancer in nude mice by a fully human single chain antibody against associated antigen ts7tmr between a549 cells and trichinella spiralis |
| topic | Trichinella spiralis A549 lung cancer associated antigens Ts7TMR scFv |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2024.2347377 |
| work_keys_str_mv | AT taotaoyue theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT jinpengwang theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT fangliu theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT pengtaogong theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT jianhuali theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT xichenzhang theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT nanzhang theeffectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT taotaoyue effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT jinpengwang effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT fangliu effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT pengtaogong effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT jianhuali effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT xichenzhang effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis AT nanzhang effectsofantilungcancerinnudemicebyafullyhumansinglechainantibodyagainstassociatedantigents7tmrbetweena549cellsandtrichinellaspiralis |