Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients

Background: Infection by Helicobacter pylori (H. pylori) is prevalent and linked to many pathological conditions. Numerous studies have explored the association between H. pylori and gastric diseases. However, there are fewer studies on the impact of H. pylori on biochemical and hematological parame...

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Main Authors: Hany Desouky, Nashwa Rizk, Huda Said, Reham Abd El-Azeem
Format: Article
Language:English
Published: Zagazig University, Faculty of Medicine 2024-11-01
Series:Microbes and Infectious Diseases
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Online Access:https://mid.journals.ekb.eg/article_371598_be42f74e64df1c1588e7ed0aebc5d477.pdf
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author Hany Desouky
Nashwa Rizk
Huda Said
Reham Abd El-Azeem
author_facet Hany Desouky
Nashwa Rizk
Huda Said
Reham Abd El-Azeem
author_sort Hany Desouky
collection DOAJ
description Background: Infection by Helicobacter pylori (H. pylori) is prevalent and linked to many pathological conditions. Numerous studies have explored the association between H. pylori and gastric diseases. However, there are fewer studies on the impact of H. pylori on biochemical and hematological parameters. Furthermore, the role of genetic variations in mediating these hematobiochemical alterations remains poorly unrevealed. Objective: explore the association between Glutathione S-transferase mu1 and theta1 (GST M1, T1) gene polymorphisms and hematobiochemical changes in H. pylori-infected individuals. Subjects and Methods: Thirty H. pylori-infected male patients and thirty healthy uninfected males as control were enrolled in the study. Biochemical profiles, oxidative stress markers, and hematological profile were measured, along with glutathione S-transferase mu1 and theta1genotyping. Statistical analyses: The statistics were conducted using the SPSS program (version 27). Results: H. pylori-infected patients had significantly decreased mean levels of many parameters like hemoglobin. Meanwhile significantly increased levels of other parameters like monocytes in comparison to the non-infected control. Additionally, patients with the null genotype for mu1 and theta1 (double null) had significantly higher levels of monocytes and other parameters. However, they had reduced total glutathione S-transferase enzyme activity compared to the control group. Conclusion: This study offers proof of hematobiochemical alterations related to infection with H. pylori and underscore the modulatory role of GSTM1/GSTT1 genetic variation with a higher susceptibility in null genotypes carriers.
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spelling doaj-art-ae1b2e29676247a89666a4ff2cdd05e32024-12-21T21:03:54ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-11-01541567157910.21608/mid.2024.297322.1997371598Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patientsHany Desouky0Nashwa Rizk1Huda Said2Reham Abd El-Azeem3Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, EgyptDepartment of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, EgyptClinical pathology Department, Faculty of medicine, Zagazig University, Zagazig, EgyptDepartment of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, EgyptBackground: Infection by Helicobacter pylori (H. pylori) is prevalent and linked to many pathological conditions. Numerous studies have explored the association between H. pylori and gastric diseases. However, there are fewer studies on the impact of H. pylori on biochemical and hematological parameters. Furthermore, the role of genetic variations in mediating these hematobiochemical alterations remains poorly unrevealed. Objective: explore the association between Glutathione S-transferase mu1 and theta1 (GST M1, T1) gene polymorphisms and hematobiochemical changes in H. pylori-infected individuals. Subjects and Methods: Thirty H. pylori-infected male patients and thirty healthy uninfected males as control were enrolled in the study. Biochemical profiles, oxidative stress markers, and hematological profile were measured, along with glutathione S-transferase mu1 and theta1genotyping. Statistical analyses: The statistics were conducted using the SPSS program (version 27). Results: H. pylori-infected patients had significantly decreased mean levels of many parameters like hemoglobin. Meanwhile significantly increased levels of other parameters like monocytes in comparison to the non-infected control. Additionally, patients with the null genotype for mu1 and theta1 (double null) had significantly higher levels of monocytes and other parameters. However, they had reduced total glutathione S-transferase enzyme activity compared to the control group. Conclusion: This study offers proof of hematobiochemical alterations related to infection with H. pylori and underscore the modulatory role of GSTM1/GSTT1 genetic variation with a higher susceptibility in null genotypes carriers.https://mid.journals.ekb.eg/article_371598_be42f74e64df1c1588e7ed0aebc5d477.pdfhelicobacter pylorihematobiochemical alterationsgst genes polymorphisms
spellingShingle Hany Desouky
Nashwa Rizk
Huda Said
Reham Abd El-Azeem
Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
Microbes and Infectious Diseases
helicobacter pylori
hematobiochemical alterations
gst genes polymorphisms
title Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
title_full Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
title_fullStr Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
title_full_unstemmed Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
title_short Impact of GST (M1, T1) gene polymorphisms on hematobiochemical changes in Helicobacter pylori-infected patients
title_sort impact of gst m1 t1 gene polymorphisms on hematobiochemical changes in helicobacter pylori infected patients
topic helicobacter pylori
hematobiochemical alterations
gst genes polymorphisms
url https://mid.journals.ekb.eg/article_371598_be42f74e64df1c1588e7ed0aebc5d477.pdf
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