Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana
Introduction: Antimicrobial resistance (AMR) has become a global public health threat, with colistin emerging as a last-resort treatment option for multidrug-resistant Gram-negative infections. However, the emergence of colistin resistance, mediated by mechanisms like mutations in the PhoP gene, rai...
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Elsevier
2024-11-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024156642 |
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| author | Emmanuel U. Osisiogu Bhavana Singh Patrick K. Feglo Kwabena O. Duedu |
| author_facet | Emmanuel U. Osisiogu Bhavana Singh Patrick K. Feglo Kwabena O. Duedu |
| author_sort | Emmanuel U. Osisiogu |
| collection | DOAJ |
| description | Introduction: Antimicrobial resistance (AMR) has become a global public health threat, with colistin emerging as a last-resort treatment option for multidrug-resistant Gram-negative infections. However, the emergence of colistin resistance, mediated by mechanisms like mutations in the PhoP gene, raises concerns about the future utility of this antibiotic. This study aimed to determine the prevalence of PhoP-mediated colistin resistance in Gram-negative bacteria isolated from the stool of residents in the Ho Municipality, Ghana. Methods: In this cross-sectional study, 110 stool samples were collected from June 2021 to December 2022. Gram-negative bacteria were isolated, and colistin susceptibility was determined by broth microdilution. Genomic DNA from resistant isolates was extracted and sequenced using the Nanopore platform to detect the presence of the PhoP gene. Results: Of the 107 Gram-negative isolates, 57 % were resistant to colistin. The PhoP gene was detected in 61.4 % of the colistin-resistant isolates, with the highest prevalence observed in Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Conclusion: The study reveals a high prevalence of PhoP-mediated colistin resistance among Gram-negative bacteria colonizing residents in the Ho Municipality, highlighting the role of the gut microbiota as a reservoir for antibiotic resistance. Continued surveillance and a collaborative One Health approach are crucial to address this growing threat. |
| format | Article |
| id | doaj-art-add5dd5d5d004ed9a4e75eb01086f558 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
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| spelling | doaj-art-add5dd5d5d004ed9a4e75eb01086f5582024-11-15T06:13:03ZengElsevierHeliyon2405-84402024-11-011021e39633Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, GhanaEmmanuel U. Osisiogu0Bhavana Singh1Patrick K. Feglo2Kwabena O. Duedu3Department of Clinical Microbiology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Department of Science Laboratory Technology, Faculty of Applied Science and Technology, Dr Hilla Limann Technical University, Wa, Ghana; Corresponding author. Department of Clinical Microbiology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Clinical Microbiology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana; Department of Paediatrics, University Health Services, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Clinical Microbiology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaCollege of Life Sciences, Faculty of Health, Education and Life Sciences, Birmingham City University, Birmingham, United Kingdom; Department of Biomedical Science, School of Basic and Biomedical Science, University of Health and Allied Sciences, Ho, GhanaIntroduction: Antimicrobial resistance (AMR) has become a global public health threat, with colistin emerging as a last-resort treatment option for multidrug-resistant Gram-negative infections. However, the emergence of colistin resistance, mediated by mechanisms like mutations in the PhoP gene, raises concerns about the future utility of this antibiotic. This study aimed to determine the prevalence of PhoP-mediated colistin resistance in Gram-negative bacteria isolated from the stool of residents in the Ho Municipality, Ghana. Methods: In this cross-sectional study, 110 stool samples were collected from June 2021 to December 2022. Gram-negative bacteria were isolated, and colistin susceptibility was determined by broth microdilution. Genomic DNA from resistant isolates was extracted and sequenced using the Nanopore platform to detect the presence of the PhoP gene. Results: Of the 107 Gram-negative isolates, 57 % were resistant to colistin. The PhoP gene was detected in 61.4 % of the colistin-resistant isolates, with the highest prevalence observed in Proteus mirabilis, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Conclusion: The study reveals a high prevalence of PhoP-mediated colistin resistance among Gram-negative bacteria colonizing residents in the Ho Municipality, highlighting the role of the gut microbiota as a reservoir for antibiotic resistance. Continued surveillance and a collaborative One Health approach are crucial to address this growing threat.http://www.sciencedirect.com/science/article/pii/S2405844024156642Colistin resistancePhoPGram-negative bacteriaGut microbiotaNanopore sequencing |
| spellingShingle | Emmanuel U. Osisiogu Bhavana Singh Patrick K. Feglo Kwabena O. Duedu Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana Heliyon Colistin resistance PhoP Gram-negative bacteria Gut microbiota Nanopore sequencing |
| title | Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana |
| title_full | Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana |
| title_fullStr | Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana |
| title_full_unstemmed | Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana |
| title_short | Detection of PhoP-mediated colistin resistance in Gram-negative bacteria without mcr genes in human population in the Ho Municipality, Ghana |
| title_sort | detection of phop mediated colistin resistance in gram negative bacteria without mcr genes in human population in the ho municipality ghana |
| topic | Colistin resistance PhoP Gram-negative bacteria Gut microbiota Nanopore sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024156642 |
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